Abstract:
:Shotgun proteomics is a high throughput technique for protein identification able to identify up to several thousand proteins from a single sample. In order to make sense of this large amount of data, proteomics analysis software is needed, aimed at making the data intuitively accessible to beginners as well as experienced scientists. This chapter provides insight on where to start when analyzing shotgun proteomics data, with a focus on explaining the most common pitfalls in protein identification analysis and how to avoid them. Finally, the move to seeing beyond the list of identified proteins and to putting the results into a bigger biological context is discussed.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Lereim RR,Oveland E,Berven FS,Vaudel M,Barsnes Hdoi
10.1007/978-3-319-41448-5_11subject
Has Abstractpub_date
2016-01-01 00:00:00pages
227-235eissn
0065-2598issn
2214-8019journal_volume
919pub_type
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