Visualization, Inspection and Interpretation of Shotgun Proteomics Identification Results.

Abstract:

:Shotgun proteomics is a high throughput technique for protein identification able to identify up to several thousand proteins from a single sample. In order to make sense of this large amount of data, proteomics analysis software is needed, aimed at making the data intuitively accessible to beginners as well as experienced scientists. This chapter provides insight on where to start when analyzing shotgun proteomics data, with a focus on explaining the most common pitfalls in protein identification analysis and how to avoid them. Finally, the move to seeing beyond the list of identified proteins and to putting the results into a bigger biological context is discussed.

journal_name

Adv Exp Med Biol

authors

Lereim RR,Oveland E,Berven FS,Vaudel M,Barsnes H

doi

10.1007/978-3-319-41448-5_11

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

227-235

eissn

0065-2598

issn

2214-8019

journal_volume

919

pub_type

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