Detection of Carbofuran-Protein Adducts in Serum of Occupationally Exposed Pesticide Factory Workers in Pakistan.


:This study was conducted to investigate the protein adducts with pesticides in a cohort of 172 factory workers that were exposed to a mixture of pesticides. The 35 samples showing considerable variation in biochemical parameters, i.e., butyrylcholinestrase (BChE), serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), gamma-glutamyl transferase (GGT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP/ALKP), lactate dehydrogenase (LDH), creatine phosphokinase (CPK) enzymes, and controls were analyzed by reversed-phase nanoscale liquid chromatography tandem mass spectrometry (nLC-MS/MS) on an Orbitrap mass spectrometer employing a shotgun proteomics approach. Only protein adducts with carbofuran were found on serum proteins of these workers. These adducts were of carbofuran labeled lysine (Lys-142, Lys-183, Lys-287, and Lys-467), arginine (Arg-210, Arg-242, and Arg-256) from serum albumin, and serine (Ser-07, Ser-54, and Ser-150) from immunoglobulin proteins. The arginine residues (Arg-210, Arg-242, Arg-246, and Arg-434) from albumin were also found to be glycated in serum of workers showing a high level of glucose who also had glycated arginine (Arg-1120) modified with carbofuran in their tankyrase-1-binding protein. The number of tandem mass spectra of modified peptides increased with increasing time of exposure. This is the first report to demonstrate the presence of carbofuran-labeled albumin, immunoglobulin, and glycated arginine, which shows that lysine and arginine of human albumin and serine of immunoglobulin are covalently modified in the serum of workers that were occupationally exposed to carbofuran, and the modification is detectable by tandem mass spectrometry. These peptides modified with carbofuran can potentially be used as a biomarker of carbofuran exposure.


Chem Res Toxicol


Rehman T,Khan MM,Shad MA,Hussain M,Oyler BL,Goo YA,Goodlett DR




Has Abstract


2016-10-17 00:00:00












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    authors: Loeppky RN,Srinivasan A

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    authors: Ponniah M,Billett EE,De Girolamo LA

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Mehta P,Church K,Williams J,Chen FX,Encell L,Shuker DE,Gold B

    更新日期:1996-09-01 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Delnomdedieu M,Basti MM,Styblo M,Otvos JD,Thomas DJ

    更新日期:1994-09-01 00:00:00

  • 42,43,44,45,46,47,55-Heptanor-41-oxohomoyessotoxin, a new biotoxin from mussels of the northern Adriatic sea.

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    authors: Ciminiello P,Fattorusso E,Forino M,Poletti R

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    authors: Arenas A,López-Alarcón C,Kogan M,Lissi E,Davies MJ,Silva E

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    pub_type: 杂志文章


    authors: Frelon S,Douki T,Favier A,Cadet J

    更新日期:2003-02-01 00:00:00

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    journal_title:Chemical research in toxicology

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    authors: Wang M,Lao Y,Cheng G,Shi Y,Villalta PW,Hecht SS

    更新日期:2007-04-01 00:00:00

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    pub_type: 杂志文章


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    更新日期:2000-02-01 00:00:00

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    authors: Rothfuss A,Steger-Hartmann T,Heinrich N,Wichard J

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    更新日期:1998-04-01 00:00:00

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    authors: Maddox JF,Roth RA,Ganey PE

    更新日期:2003-05-01 00:00:00

  • Bisphenol A activates the Nrf1/2-antioxidant response element pathway in HEK 293 cells.

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Roberts-Kirchhoff ES,Crowley JR,Hollenberg PF,Kim H

    更新日期:1999-07-01 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


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    更新日期:1994-01-01 00:00:00

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    更新日期:2013-12-16 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Devanesan P,Ariese F,Jankowiak R,Small GJ,Rogan EG,Cavalieri EL

    更新日期:1999-09-01 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Mohammadi-Bardbori A,Omidi M,Arabnezhad MR

    更新日期:2019-04-15 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Pathira Kankanamge LS,Perera HM,Griffin WC

    更新日期:2021-01-28 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Liu H,Liu J,van Breemen RB,Thatcher GR,Bolton JL

    更新日期:2005-02-01 00:00:00

  • (-)-Epigallocatechin gallate, a major constituent of green tea, poisons human type II topoisomerases.

    abstract::(-)-Epigallocatechin gallate (EGCG) is the most abundant and biologically active polyphenol in green tea, and many of the therapeutic benefits of the beverage have been attributed to this compound. High concentrations of EGCG are cytotoxic and trigger genotoxic events in mammalian cells. Although this catechin affects...

    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Bandele OJ,Osheroff N

    更新日期:2008-04-01 00:00:00