Abstract:
CONCLUSIONS:The present study revealed that miR-21 promotes the proliferation and invasion of cholesteatoma keratinocytes. These results provide a partial explanation for the more aggressive clinical behavior observed in cholesteatoma. OBJECTIVE:This study aims to investigate the post-transcriptional regulatory effects that control proliferation, apoptosis, and invasion in cholesteatoma keratinocytes. In particular, the potential role of miR-21 was focused on in this study. METHODS:Thirty cholesteatoma tissues were processed for RNA and cell culture. Cholesteatoma keratinocytes were transfected with miR-21 mimics, miR-21 inhibitors, or negative control miRNAs; and growth curves were drawn. RT-PCR was used to assess the expression levels of miR-21. EdU incorporation assay and TUNEL staining were used to assess the proliferation and apoptosis of cholesteatoma keratinocytes, respectively. The invasive abilities of cholesteatoma keratinocytes were examined using 6-well Transwell plates. RESULTS:MiRNA-21 was upregulated when cholesteatoma keratinocytes were transfected with miR-21 mimics. Furthermore, the number of proliferative EdU + cells increased in cholesteatoma keratinocytes transfected with miR-21 mimics; and the number of TUNEL-positive cells also increased in cells transfected with miR-21 mimics. In addition, the number of migrated cells transfected with miR-21 mimics was higher, compared with migrated cells transfected miR-21 inhibitors or control miRNA.
journal_name
Acta Otolaryngoljournal_title
Acta oto-laryngologicaauthors
Chen X,Li X,Qin Zdoi
10.1080/00016489.2016.1202447subject
Has Abstractpub_date
2016-12-01 00:00:00pages
1261-1266issue
12eissn
0001-6489issn
1651-2251journal_volume
136pub_type
杂志文章abstract:CONCLUSION:There is no increased risk of postoperative haemorrhage for abscess tonsillectomies in comparison to elective tonsillectomies. OBJECTIVE:There is still controversy as regards the optimal management of peritonsillar abscess. Opponents of tonsillectomy à chaud cite an increased postoperative bleeding risk. Mo...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480510012336
更新日期:2005-12-01 00:00:00
abstract::A prospective study was carried out to assess the possible biochemical background of the AAm production by HVF in accordance with selected histological types and voice disorders. The mass of new tissue forming HVF in different clinical cases led us to suspect, according to our previous results, that PGs, LTs and other...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489309135840
更新日期:1993-05-01 00:00:00
abstract:CONCLUSION:It is thought that oxidative stress may be the major cause of the increase in the oxide thiol form in the study group. The relationship between oxidative stress status and dynamic thiol/disulphide in nasal polyposis now needs to be investigated. OBJECTIVES:The purpose of this study was to evaluate the relat...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2016.1186833
更新日期:2016-11-01 00:00:00
abstract::Malignant eccrine poroma was first described by Pinkus and Mehregon in 1963. Because of the histologic characteristics of the tumor, these authors concluded that the tumor was likely of eccrine origin. Malignant eccrine poroma originates from the intraepidermal ductal portion of the eccrine gland. Like other tumors of...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480500395187
更新日期:2006-04-01 00:00:00
abstract::The activity of central vestibular neurons (Vn) of the horizontal canal system was recorded in chronically hemilabyrinthectomized cats and compared with that of labyrinth intact animals. In both groups the cerebellar vermis was removed in order to assess the efficacy of the vestibular brainstem commissure alone by mea...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488409107529
更新日期:1984-07-01 00:00:00
abstract::The gram negative bacteria, nontypable Haemophilus influenzae (NTHi) was used to induce otitis media in a total of 18 chinchillas. Three days post-inoculation, three cohorts of 6 chinchillas each were treated daily for four days with either ceftriaxone, chloramphenicol, or diluent without antibiotics. Middle ear fluid...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489709118000
更新日期:1997-01-01 00:00:00
abstract::We established new cell lines from head and neck cancer patients for studies of adhesion molecules and cellular behavior in nine patients with primary or metastatic cancer treated at the Asan Medical Center. Explant cultures of fresh tumor tissue were used to develop new permanent tumor cell lines. Lines were tested f...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489709113477
更新日期:1997-09-01 00:00:00
abstract::The records of 627 patients with inflammatory disease of the tonsils and 310 patients undergoing dissection tonsillectomy are analysed. The majority of the tonsil population in our study group are adults, and there is a high incidence of rheumatic fever and rheumatic heart disease. The study population is apt to recei...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488309132739
更新日期:1983-11-01 00:00:00
abstract:CONCLUSION:Reconstruction with a vascularized bone flap provided superior postoperative outcomes compared with reconstruction with a soft tissue free flap. However, patients obtained acceptable long-term functional outcomes even with a soft tissue free flap and this method is an option in selected patients. A multidisc...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2013.824111
更新日期:2013-12-01 00:00:00
abstract:CONCLUSION:The incidence of new cases of Meniere's disease (MD) in elderly patients aged 60 years or more was found to have increased over time after correction for age distribution in the overall population. Job- and care-related fatigue may be involved in the recent increase in elderly-onset cases because physical an...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章,多中心研究
doi:10.1080/00016480802112587
更新日期:2009-02-01 00:00:00
abstract::The vestibular and optokinetic responses of a group of white cats were evaluated and compared with the responses from a control group of pigmented animals. The results indicate that in all cases the white cats exhibited varying degrees of vestibular and/or optokinetic dysfunction, which in some animals varied from tes...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016487709123944
更新日期:1977-07-01 00:00:00
abstract::Although doxorubicin [adriamycin (ADM)] ototoxicity has not been detected to date, it has been reported that neurotoxicity in the central nervous system was induced by chemotherapy with ADM in patients receiving chronic cyclosporin A (CsA) treatment. ADM ototoxicity may be induced by combination therapy with CsA becau...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480152602212
更新日期:2001-10-01 00:00:00
abstract:CONCLUSION:The Finnish Matrix Test is the first sentence test in noise for the Finnish language. It was developed according to the HearCom standards and provides reliable speech intelligibility measurements with highly comparable results with the other international matrix tests. OBJECTIVES:The aim of the study was to...
journal_title:Acta oto-laryngologica
pub_type: 临床试验,杂志文章
doi:10.3109/00016489.2014.898185
更新日期:2014-07-01 00:00:00
abstract::Experimental animals (cats) were exposed to tones of 125, 1000, 2000, and 4000 Hz at sound pressure levels in the range 120 to 157.5 dB, and for durations of one hour (1000, 2000, 4000 Hz) or four hours (125 Hz). Pure tone audiograms were obtained for each animal before and after exposure. Post-exposure tests were con...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016487509121336
更新日期:1975-11-01 00:00:00
abstract::A total of 33 consecutively operated aural cholesteatomas were analysed with regard to preoperative findings and correlated with the morphology of the cholesteatoma tissue. Patients were found in all age groups, 9/33 of the patients were operated at an age below 15 years. A persistent ear discharge had occurred in all...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:
更新日期:1981-03-01 00:00:00
abstract::Background: Recurrent respiratory papillomatosis (RRP) remains a challenging and frustrating disease to treat.Objective: To explore the efficacy of microsurgery in combined with Topical-PDT in treating recurrent respiratory papillomatosis.Materials and methods: Fifty patients with RRP were treated with microsurgery in...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2019.1667530
更新日期:2019-12-01 00:00:00
abstract:CONCLUSIONS:1. The principle of bilateral symmetry depends on the chordal cartilage that is the keystone in cranial base ossification in rats and humans, due to its anatomical situation and for the production of the chordin protein that regulates the bone morphogenetic protein BMP-7. 2. In humans and in rats, foramen l...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2011.642814
更新日期:2012-04-01 00:00:00
abstract::Different analysis methods for the objective measurements of body sway were examined to establish the most sensitive method for identifying changes in stability. Changes between stability with eyes open and eyes closed were used to assess the techniques. Body sway was measured in a group of normal subjects standing on...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489409126028
更新日期:1994-03-01 00:00:00
abstract:CONCLUSION:L-N-Acetylcysteine (L-NAC) significantly reduced reactive oxygen species (ROS) generation and cochlear cell apoptosis after irradiation. The safe and effective use of L-NAC in reducing radiation-induced sensorineural hearing loss (SNHL) should be verified by further in vivo studies. OBJECTIVES:Radiation-ind...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480701762490
更新日期:2008-04-01 00:00:00
abstract::This study was designed to assess the utility of otolith-ocular reflex testing in patients with complete unilateral peripheral vestibular deficits. Ten subjects were given a clinical vestibular test battery (ENG & vertical rotation) and research oriented tests of otolith function including dynamic posturography with h...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489509139392
更新日期:1995-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the capability of indirect computed tomography and magnetic resonance lymphography (CT/MR-LG) with gadolinium-loaded, polyethylenimine-entrapped gold nanoprobes (Gd-Au PENPs) for assessing lingual sentinel lymph node (SLN) metastasis in a tongue VX2 carcinoma model. MATERIALS AND METHODS:Seventee...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2018.1441544
更新日期:2018-08-01 00:00:00
abstract:HYPOTHESIS:To examine the cellular events following induced superficial lesions of the human tympanic membrane (TM). Such information could lead to enhanced appreciation of repair mechanisms and novel strategies to restore TM perforations. BACKGROUND:Persistent perforation of the TM in chronic otitis media is a major ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2017.1321139
更新日期:2017-09-01 00:00:00
abstract::The present paper investigates the hearing function in chronic otitis media vs. tympanoplasty in 41 patients with bilateral chronic otitis media, operated on unilaterally. The auditory evaluation was performed immediately before surgery and at a follow-up time varying from 1 to 14 years (mean = 5 years). Results were ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489109138410
更新日期:1991-01-01 00:00:00
abstract::We report a case of electromagnetic interference between a bone-anchored hearing aid (BAHA) and a cellular phone. A 54-year-old women was successfully treated for severe mixed conductive and sensorineural hearing loss with a BAHA. Five years after implantation, the patient experienced a sudden feeling of dizziness, ac...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/000164800750061723
更新日期:2000-10-01 00:00:00
abstract::The occurrence of symptoms from the upper airways in the furniture industry was studied by means of a questionnaire which was answered by 676 wood furniture workers from 50 different factories. Twenty per cent reported nasal hypersecretion and 40% nasal obstruction. A randomized sample of 61 workers was investigated f...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488409107596
更新日期:1984-11-01 00:00:00
abstract:CONCLUSIONS:These findings are in line with previous data on the horizontal vestibulo-ocular reflex (VOR) from this laboratory and suggest that eye position signals do not modulate natural vestibular responses. Hence, the Alexander's law (AL) phenomenon cannot be interpreted simply as a consequence of vestibular or ocu...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2012.682120
更新日期:2012-10-01 00:00:00
abstract::The postnatal development of the Eustachian tube (ET) and its surrounding structures was investigated by means of computer-aided three-dimensional (3-D) reconstruction methods in 13 normal human temporal bones, obtained from individuals 3 months to 71 years old. The cross-sectional area, width and height of the lumen ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489850182549
更新日期:1998-11-01 00:00:00
abstract:CONCLUSION:The risk of operation-requiring postoperative bleeding after abscess tonsillectomy is no different from the risk after elective tonsillectomy. However, the risk is higher for older than for younger patients, both for the whole cohort and for tonsillectomy à chaud. OBJECTIVE:The objective of the present stud...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2012.660545
更新日期:2012-07-01 00:00:00
abstract::Metal intoxication (mercury and arsenic) in guinea pigs may cause damage to labyrinthine blood vessels by swelling of the endothelial cells, mitochondrial disintegration and sometimes protrusion of endothelial cell cytoplasm herniating into the blood vessel lumen. Chronic mercury intoxication resulted in distorted end...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016487709128869
更新日期:1977-05-01 00:00:00
abstract:CONCLUSION:The psychometric characteristics of Standard-Chinese lexical neighborhood test (LNT) confirmed the lexical effects of the four word categories. The established normative baseline can be used in evaluating the word-recognition performance of the hearing-impaired listeners. OBJECTIVES:The purpose of the prese...
journal_title:Acta oto-laryngologica
pub_type: 临床试验,杂志文章
doi:10.3109/00016489.2013.840923
更新日期:2014-01-01 00:00:00