Use of allicin as feed additive to enhance vaccination capacity of Clostridium perfringens toxoid in rabbits.


:The present study assessed the efficacy of Clostridium perfringens (C. perfringens) toxoid and/or allicin - as feed additive - in rabbits for preventing or minimizing the severity of infection with locally isolated strain of C. perfringens type A. Serum biochemical, immunological and pathological investigations were also done. One hundred rabbits of 6 weeks of age were divided into five equal groups (G1-G5). G1 were kept as normal control. G2 was allocated for C. perfringens type A infection. G3 was vaccinated with C. perfringens toxoid at zero time and then with a booster dose at the 3rd week of the experimental period. G4 was treated with allicin 20% added to the ration (200mg/kg ration) all over the experimental period. G5 was vaccinated with C. perfringens toxoid at the zero time then with a booster dose at the 3rd week of the experiment period, and treated with allicin 20% from the zero time till the end of the experiment. At the 4th week, G2, G3, G4 and G5 were challenged orally (5 ml) and subcutaneously (2 ml) with 24h cooked meat broth containing 1 × 10(7) colony-forming units/ml of C. perfringens type A strain. Blood and tissue samples were collected from all groups po st-vaccination then post-challenge for biochemical analysis, serum neutralization test and histopathological examinations. Results revealed that rabbits treated with both allicin and toxoid vaccine demonstrated high level of antitoxin titre post-challenge, improved liver and kidney functions, and reduced morbidity and mortality rates and the severity of histopathological changes associated with challenge of rabbits with C. perfringens type A strain. In conclusion, vaccination of rabbits with C. perfringens toxoid combined with allicin 20% gave better protection, enhanced immune response and had no adverse effects on the general health conditions against C. perfringens type A infection compared to rabbits vaccinated with C. perfringens toxoid only.






Abu El Hammed W,Soufy H,El-Shemy A,Nasr SM,Dessouky MI




Has Abstract


2016-04-12 00:00:00
















  • Process development for the mass production of Ehrlichia ruminantium.

    abstract::This work describes the optimization of a cost-effective process for the production of an inactivated bacterial vaccine against heartwater and the first attempt to produce the causative agent of this disease, the rickettsia Ehrlichia ruminantium (ER), using stirred tanks. In vitro, it is possible to produce ER using c...


    pub_type: 杂志文章


    authors: Marcelino I,Sousa MF,Veríssimo C,Cunha AE,Carrondo MJ,Alves PM

    更新日期:2006-03-06 00:00:00

  • Cumulative and episodic vaccine aluminum exposure in a population-based cohort of young children.

    abstract:BACKGROUND:In addition to antigens, vaccines contain small amounts of preservatives, adjuvants, and residual substances from the manufacturing process. Some parents have concerns about the safety of these ingredients, yet no large epidemiological studies have specifically examined associations between health outcomes a...


    pub_type: 杂志文章


    authors: Glanz JM,Newcomer SR,Daley MF,McClure DL,Baxter RP,Jackson ML,Naleway AL,Lugg MM,DeStefano F

    更新日期:2015-11-27 00:00:00

  • Immunogenicity of Vibrio cholerae ghosts following intraperitoneal immunization of mice.

    abstract::The immunogenic potential of Vibrio cholerae ghosts (VCG) in comparison with heat-killed whole-cell vibrios (WCV) was evaluated after intraperitoneal immunization of adult mice. Swiss white mice received four doses of VCG or WCV intraperitoneally, consisting of 500 micrograms of lyophilized material in 200 microliters...


    pub_type: 杂志文章


    authors: Eko FO,Hensel A,Bunka S,Lubitz W

    更新日期:1994-11-01 00:00:00

  • A tetravalent meningococcal serogroups A, C, W-135, and Y tetanus toxoid conjugate vaccine is immunogenic and well-tolerated when co-administered with Twinrix(®) in subjects aged 11-17 years: an open, randomised, controlled trial.

    abstract::The co-administration of the tetravalent meningococcal conjugate vaccine, MenACWY-TT, with a licensed hepatitis A and B vaccine, HepA/B (Twinrix(®)), was compared to their separate administration in this open, randomised, controlled study. Healthy subjects 11-17 years of age (n=611) were randomised (3:1:1) to receive ...


    pub_type: 杂志文章,随机对照试验


    authors: Østergaard L,Silfverdal SA,Berglund J,Flodmark CE,West C,Bianco V,Baine Y,Miller JM

    更新日期:2012-01-17 00:00:00

  • A ML29 reassortant virus protects guinea pigs against a distantly related Nigerian strain of Lassa virus and can provide sterilizing immunity.

    abstract::Lassa virus (LASV) is responsible for the deaths of thousands of people in West Africa annually. Genetic diversity among LASV strains is the highest among the Arenaviridae and represents a great challenge for vaccine development. Guinea pigs vaccinated with a ML29 reassortant vaccine experienced sterilizing immunity a...


    pub_type: 杂志文章


    authors: Carrion R Jr,Patterson JL,Johnson C,Gonzales M,Moreira CR,Ticer A,Brasky K,Hubbard GB,Moshkoff D,Zapata J,Salvato MS,Lukashevich IS

    更新日期:2007-05-16 00:00:00

  • Tetravalent dengue DIIIC protein together with alum and ODN elicits a Th1 response and neutralizing antibodies in mice.

    abstract::Dengue disease is a global challenge for healthcare systems particularly during outbreaks, and millions of dollars are spent every year for vector control. An efficient and safe vaccine that is cost-effective could resolve the burden that dengue virus imposes on affected countries. We describe here the immunogenicity ...


    pub_type: 杂志文章


    authors: Zuest R,Valdes I,Skibinski D,Lin Y,Toh YX,Chan K,Hermida L,Connolly J,Guillen G,Fink K

    更新日期:2015-03-17 00:00:00

  • Construction and characterisation of O139 cholera vaccine candidates.

    abstract::The hemagglutinin/protease (HA/P) seems to be an attractive locus for the insertion of heterologous tags in live cholera vaccine strains. A deltaCTXphi spontaneous mutant derived from a pathogenic strain of O139 Vibrio cholerae was sequentially manipulated to obtain hapA Colon, two colons celA derivatives which were l...


    pub_type: 杂志文章


    authors: Ledón T,Valle E,Valmaseda T,Cedré B,Campos J,Rodríguez BL,Marrero K,García H,García L,Fando R

    更新日期:2003-03-07 00:00:00

  • Identification of Mycobacterium tuberculosis vaccine candidates using human CD4+ T-cells expression cloning.

    abstract::To identify Mycobacterium tuberculosis (Mtb) antigens as candidates for a subunit vaccine against tuberculosis (TB), we have employed a CD4+ T-cell expression screening method. Mtb-specific CD4+ T-cell lines from nine healthy PPD positive donors were stimulated with different antigenic substrates including autologous ...


    pub_type: 杂志文章


    authors: Coler RN,Dillon DC,Skeiky YA,Kahn M,Orme IM,Lobet Y,Reed SG,Alderson MR

    更新日期:2009-01-07 00:00:00

  • Reduction of foot-and-mouth disease (FMD) virus load in nasal excretions, saliva and exhaled air of vaccinated pigs following direct contact challenge.

    abstract::In future, a policy of "vaccinate-to-live" may be included in the repertoire of foot-and-mouth disease (FMD) control measures and in support of this approach, we have investigated the hypothesis that vaccine-induced reduction in virus replication and excretion from pigs can be correlated to the severity of clinical si...


    pub_type: 杂志文章


    authors: Parida S,Fleming L,Oh Y,Mahapatra M,Hamblin P,Gloster J,Doel C,Gubbins S,Paton DJ

    更新日期:2007-11-07 00:00:00

  • A nationwide vaccination programme in Italy against hepatitis B virus infection in infants of hepatitis B surface antigen-carrier mothers.

    abstract::In order to prevent hepatitis B virus (HBV) infection of infants of hepatitis B surface antigen (HBsAg)-positive mothers, a nation-wide immunization programme was initiated in Italy in January 1984. During the first 3 years (1984-86), 651,667 out of 1726,000 pregnant women (37.8%) were screened for HBsAg; the percenta...


    pub_type: 杂志文章


    authors: Stroffolini T,Pasquini P,Mele A

    更新日期:1989-04-01 00:00:00

  • Safety, immunogenicity, and lot-to-lot consistency of a quadrivalent inactivated influenza vaccine in children, adolescents, and adults: A randomized, controlled, phase III trial.

    abstract:BACKGROUND:Inactivated quadrivalent influenza vaccine (IIV4) containing two influenza A strains and one strain from each B lineage (Yamagata and Victoria) may offer broader protection against seasonal influenza than inactivated trivalent influenza vaccine (IIV3), containing a single B strain. This study examined the sa...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Cadorna-Carlos JB,Nolan T,Borja-Tabora CF,Santos J,Montalban MC,de Looze FJ,Eizenberg P,Hall S,Dupuy M,Hutagalung Y,Pépin S,Saville M

    更新日期:2015-05-15 00:00:00

  • The inactivated Cowdria ruminantium vaccine for heartwater protects against heterologous strains and against laboratory and field tick challenge.

    abstract::We previously described that an inactivated vaccine against heartwater prepared from Cowdria ruminantium (Crystal Springs strain) organisms and administered in complete Freund's adjuvant (cFA) protected sheep against homologous needle challenge. Further studies, described herein, demonstrated that this vaccine protect...


    pub_type: 杂志文章


    authors: Mahan SM,Kumbula D,Burridge MJ,Barbet AF

    更新日期:1998-07-01 00:00:00

  • Vaxvec: The first web-based recombinant vaccine vector database and its data analysis.

    abstract::A recombinant vector vaccine uses an attenuated virus, bacterium, or parasite as the carrier to express a heterologous antigen(s). Many recombinant vaccine vectors and related vaccines have been developed and extensively investigated. To compare and better understand recombinant vectors and vaccines, we have generated...


    pub_type: 杂志文章


    authors: Deng S,Martin C,Patil R,Zhu F,Zhao B,Xiang Z,He Y

    更新日期:2015-11-27 00:00:00

  • Changes in meningococcal C epidemiology and vaccine effectiveness after vaccine introduction and schedule modification.

    abstract:PURPOSE:Meningococcal C conjugate vaccine was included in December 2000 in the Spanish childhood vaccination at 2, 4 and 6 months of age. In 2006, routine vaccination was modified to two doses at 2 and 4-6 months and a booster dose during the second year of age. Additionally, successive catch-up campaigns were launched...


    pub_type: 杂志文章


    authors: Garrido-Estepa M,León-Gómez I,Herruzo R,Cano R

    更新日期:2014-05-07 00:00:00

  • One-year study of the 2-1-1 intramuscular postexposure rabies vaccine regimen in 100 severely exposed Thai patients using rabies immune globulin and Vero cell rabies vaccine.

    abstract::The 2-1-1 rabies postexposure treatment schedule is an abbreviated regimen in which a tissue culture rabies vaccine is administered intramuscularly at two sites on day 0, and at one site on days 7 and 21. Compared to the standard five-dose intramuscular regimen, the 2-1-1 schedule reduces the number of clinic visits f...


    pub_type: 杂志文章


    authors: Chutivongse S,Wilde H,Fishbein DB,Baer GM,Hemachudha T

    更新日期:1991-08-01 00:00:00

  • Efficacy and immunogenicity of high-dose influenza vaccine in older adults by age, comorbidities, and frailty.

    abstract:BACKGROUND:A randomized trial demonstrated that a high-dose inactivated influenza vaccine (IIV-HD) was 24.2% more efficacious than a standard-dose vaccine (IIV-SD) against laboratory-confirmed influenza illness in adults ≥65 years. To evaluate the consistency of IIV-HD benefits, supplemental analyses explored efficacy ...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: DiazGranados CA,Dunning AJ,Robertson CA,Talbot HK,Landolfi V,Greenberg DP

    更新日期:2015-08-26 00:00:00

  • Post-exposure rabies prophylaxis: establishing effectiveness of abbreviated treatments.

    abstract::Several publications have reported recently the apparently successful use of human diploid cell rabies vaccine (HDCV) in volumes of less than the 6 X 1.0 cm3 dosage schedule currently recommended by the World Health Organization for post-exposure rabies prophylaxis. The newer proposed treatments are advocated to econo...


    pub_type: 杂志文章


    authors: Gross EM,Bearman JE

    更新日期:1985-09-01 00:00:00

  • Evaluation of Japanese encephalitis virus E and NS1 proteins immunogenicity using a recombinant Newcastle disease virus in mice.

    abstract::Japanese encephalitis (JE) is the most important cause of acute encephalitis syndrome (AES). Japanese encephalitis virus (JEV), the prototype member of the JE serocomplex, belongs to the genus Flavivirus. The immunogenic proteins envelope (E) and non-structural protein 1 (NS1) of JEV are widely explored for the develo...


    pub_type: 杂志文章


    authors: Nath B,Vandna,Saini HM,Prasad M,Kumar S

    更新日期:2020-02-11 00:00:00

  • The Global Meningococcal Initiative: recommendations for reducing the global burden of meningococcal disease.

    abstract::The Global Meningococcal Initiative (GMI) is composed of an international group of scientists, clinicians and public health officials with expertise in meningococcal immunology, epidemiology and prevention. The primary goal of the GMI is the promotion of the global prevention of invasive meningococcal disease through ...


    pub_type: 杂志文章


    authors: Harrison LH,Pelton SI,Wilder-Smith A,Holst J,Safadi MA,Vazquez JA,Taha MK,LaForce FM,von Gottberg A,Borrow R,Plotkin SA

    更新日期:2011-04-18 00:00:00

  • Hepatitis A shifting epidemiology in Latin America.

    abstract::In the past, Latin America was considered to be an area of high endemicity for hepatitis A virus (HAV) infection, with most people infected in early childhood. A seroepidemiological study was recently undertaken in six countries to determine whether this pattern has changed. The highest seroprevalence of antibodies to...


    pub_type: 杂志文章


    authors: Tanaka J

    更新日期:2000-02-18 00:00:00

  • The effect of immunization on chemokines and CCR5 and CXCR4 coreceptor functions in SIV binding and chemotaxis.

    abstract::The replication of simian immunodeficiency virus (SIV) in acutely infected CD4+ cells can be inhibited in vitro by CD8-suppressor factors (SF) and beta-chemokines induced by immunization of macaques with SIV gp120 and p27 in Alum. A comparison between intradermal, naso-rectal-i.m. and targeted iliac lymph node (TILN) ...


    pub_type: 杂志文章


    authors: Wang Y,Tao L,Mitchell E,Bergmeier L,Doyle C,Lehner T

    更新日期:1999-04-09 00:00:00

  • The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation.

    abstract::We investigated the influence of the site of administration of DNA vaccine on the induced immune response. DNA vaccines were administered by electroporation at three different sites: tibial cranial muscle, abdominal skin and ear pinna. Aiming to draw general conclusions about DNA vaccine delivery, we successively used...


    pub_type: 杂志文章


    authors: Vandermeulen G,Vanvarenberg K,De Beuckelaer A,De Koker S,Lambricht L,Uyttenhove C,Reschner A,Vanderplasschen A,Grooten J,Préat V

    更新日期:2015-06-22 00:00:00

  • Macrophage-derived insulin-like growth factor-1 affects influenza vaccine efficacy through the regulation of immune cell homeostasis.

    abstract::The level of antibody production induced by a vaccine involves a variety of host factors. One of these, insulin-like growth factor-1 (IGF-1), plays an important role in lymphocyte maturation and antibody expression. Here, we investigated the role of macrophage-derived IGF-1 in the induction of influenza vaccine-specif...


    pub_type: 杂志文章


    authors: Yoon IS,Park H,Kwak HW,Woo Jung Y,Nam JH

    更新日期:2017-08-24 00:00:00

  • Vaccination markers: designing unique antigens to be added to vaccines to differentiate between natural infection and vaccination.

    abstract::Some important vaccines, including ones against tuberculosis (Tb) and foot-and-mouth disease (FMD), are not used in many developed countries because there is no simple way to differentiate between vaccine-induced immunity and infection. Eliminating major protein antigens has not succeeded in the cases of Tb, FMD and s...


    pub_type: 杂志文章


    authors: Root-Bernstein RS

    更新日期:2005-03-18 00:00:00

  • Vaccination with recombinant Plasmodium vivax MSP-10 formulated in different adjuvants induces strong immunogenicity but no protection.

    abstract::Although largely considered benign, Plasmodium vivax causes disease in nearly 75 million people each year and the available strategies are not sufficient to reduce the burden of disease, therefore pointing to vaccine development as a cost-effective control measure. In this study, the P. vivax merozoite surface protein...


    pub_type: 杂志文章


    authors: Giraldo MA,Arevalo-Pinzon G,Rojas-Caraballo J,Mongui A,Rodriguez R,Patarroyo MA

    更新日期:2009-12-10 00:00:00

  • Varicella vaccination as useful strategy for reducing the risk of varicella-related hospitalizations in both vaccinated and unvaccinated cohorts (Italy, 2003-2018).

    abstract:INTRODUCTION:The present study summarizes evidences of the impact that varicella vaccination (VV) introduction and coverage can have on varicella attributable hospitalization rates. METHODS:A retrospective observational study was carried out by considering hospital discharge records and VV coverage at 24 months collec...


    pub_type: 杂志文章


    authors: Amodio E,Casuccio A,Tramuto F,Costantino C,Marrella A,Maida CM,Vitale F,Restivo V

    更新日期:2020-07-31 00:00:00

  • Routing dependent immune responses after experimental R848-adjuvated vaccination.

    abstract::Most traditional vaccines are administered via the intramuscular route. Other routes of administration however, can induce equal or improved protective memory responses and might provide practical advantages such as needle-free immunization, dose sparing and induction of tissue-specific (mucosal) immunity. Here we exp...


    pub_type: 杂志文章


    authors: van Aalst S,Jansen MAA,Ludwig IS,van der Zee R,van Eden W,Broere F

    更新日期:2018-03-07 00:00:00

  • Enhanced protection against pneumococcal infection elicited by immunization with the combination of PspA, PspC, and ClpP.

    abstract::Immunization with a combination of several virulence-associated proteins is one of the strategies of developing effective protein-based vaccines to enhance the protection against Streptococcus pneumoniae. In this study, we evaluated the protection effects against pneumococcal infection caused by S. pneumoniae TIGR4 in...


    pub_type: 杂志文章


    authors: Cao J,Chen D,Xu W,Chen T,Xu S,Luo J,Zhao Q,Liu B,Wang D,Zhang X,Shan Y,Yin Y

    更新日期:2007-06-28 00:00:00

  • Critical prevalence of antibodies minimizing vaccination costs for hepatitis A, hepatitis B, varicella, measles and tetanus in adults and adolescents in Catalonia, Spain.

    abstract::The prevalence of seropositive individuals that makes costs of vaccinating all individuals equal to that for screening and vaccination of susceptible individuals is defined as the critical prevalence of antibodies (p*). Screening and vaccination is more efficient when the prevalence of seropositive individuals (p) in ...


    pub_type: 杂志文章


    authors: Plans-Rubió P

    更新日期:2004-09-28 00:00:00

  • Impact of the 10-valent pneumococcal non-typeable Haemophilus influenzae Protein D conjugate vaccine (PHiD-CV) on bacterial nasopharyngeal carriage.

    abstract::Pneumococcal conjugate vaccines (PCV) may reduce nasopharyngeal carriage (NPC) of Streptococcus pneumoniae vaccine strains (VT), but serotype replacement with non-vaccine strains (NVT) has been reported. Bacterial NPC after PHiD-CV vaccination was assessed in the second year of life. Open descriptive study of NPC repo...


    pub_type: 杂志文章


    authors: Prymula R,Hanovcova I,Splino M,Kriz P,Motlova J,Lebedova V,Lommel P,Kaliskova E,Pascal T,Borys D,Schuerman L

    更新日期:2011-02-24 00:00:00