Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk.

Abstract:

:Nisin, an important bacteriocin from Lactococcus lactis subsp., is primarily active against various Gram-positive bacteria. Leucocin C, produced by Leuconostoc carnosum 4010, is a class IIa bacteriocin used to inhibit the growth of Listeria monocytogenes. Because two bacteriocins have different modes of action, the combined use of them could be a potential strategy for effective inhibition of foodborne pathogens. In this study, L. lactis N8-r-lecCI (N8 harboring lecCI gene) coexpressing nisin-leucocin C was constructed based on the food-grade carrier L. lactis N8. Production of both bacteriocins was stably maintained. Antimicrobial measurements showed that the recombinant strain is effectively against Listeria monocytogenes and Staphylococcus aureus and moderately against Salmonella enterica serovar Enteritidis and Escherichia coli because of its stronger antibacterial activity than the parental strain, this result first demonstrated that the co-expression of nisin and leucocin C results in highly efficient antimicrobial activity. The checkerboard assay showed that the antibacterial activity of L. lactis N8-r-lecCI supernatant was enhanced in the presence of low concentration of EDTA. Analysis of the scanning electron microscope image showed the biggest cellular morphology change in L. monocytogenes treated with a mixture of EDTA and L. lactis N8-r-lecCI supernatant. The practical effect was verified in pasteurized milk through time-kill assay. The L. lactis N8-r-lecCI strain expressing both nisin and leucocin C has a promising application prospect in pasteurized milk processing and preservation because of its strong antibacterial activity.

journal_name

Front Microbiol

authors

Fu Y,Mu D,Qiao W,Zhu D,Wang X,Liu F,Xu H,Saris P,Kuipers OP,Qiao M

doi

10.3389/fmicb.2018.00547

subject

Has Abstract

pub_date

2018-03-23 00:00:00

pages

547

issn

1664-302X

journal_volume

9

pub_type

杂志文章
  • Confocal Laser Scanning Microscopy for Analysis of Pseudomonas aeruginosa Biofilm Architecture and Matrix Localization.

    abstract::Most microbes can produce surface-associated or suspended aggregates called biofilms, which are encased within a biopolymer-rich matrix. The biofilm matrix provides structural integrity to the aggregates and shields the resident cells against environmental stressors, including antibiotic treatment. Microscopy permits ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.00677

    authors: Reichhardt C,Parsek MR

    更新日期:2019-04-02 00:00:00

  • Whole Genome Sequencing of Mycobacterium tuberculosis Clinical Isolates From India Reveals Genetic Heterogeneity and Region-Specific Variations That Might Affect Drug Susceptibility.

    abstract::Whole genome sequencing (WGS) of Mycobacterium tuberculosis has been constructive in understanding its evolution, genetic diversity and the mechanisms involved in drug resistance. A large number of sequencing efforts from across the globe have revealed genetic diversity among clinical isolates and the genetic determin...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00309

    authors: Advani J,Verma R,Chatterjee O,Pachouri PK,Upadhyay P,Singh R,Yadav J,Naaz F,Ravikumar R,Buggi S,Suar M,Gupta UD,Pandey A,Chauhan DS,Tripathy SP,Gowda H,Prasad TSK

    更新日期:2019-02-26 00:00:00

  • Inhibition of HTLV-1 Infection by HIV-1 First- and Second-Generation Integrase Strand Transfer Inhibitors.

    abstract::More than 10 million people worldwide are infected with the retrovirus human T-cell lymphotropic virus type 1 (HTLV-1). Infection phenotypes can range from asymptomatic to severe adult T-cell leukemia/lymphoma (ATLL) and HTLV-1-associated myelopathy. HTLV-1, like human immunodeficiency virus type 1 (HIV-1), is a blood...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01877

    authors: Barski MS,Minnell JJ,Maertens GN

    更新日期:2019-08-13 00:00:00

  • A brief history of bacterial growth physiology.

    abstract::Arguably, microbial physiology started when Leeuwenhoek became fascinated by observing a Vorticella beating its cilia, my point being that almost any observation of microbes has a physiological component. With the advent of modern microbiology in the mid-19th century, the field became recognizably distinctive with suc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00289

    authors: Schaechter M

    更新日期:2015-04-21 00:00:00

  • Time-Resolved Transcriptomics and Constraint-Based Modeling Identify System-Level Metabolic Features and Overexpression Targets to Increase Spiramycin Production in Streptomyces ambofaciens.

    abstract::In this study we have applied an integrated system biology approach to characterize the metabolic landscape of Streptomyces ambofaciens and to identify a list of potential metabolic engineering targets for the overproduction of the secondary metabolites in this microorganism. We focused on an often overlooked growth p...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00835

    authors: Fondi M,Pinatel E,Talà A,Damiano F,Consolandi C,Mattorre B,Fico D,Testini M,De Benedetto GE,Siculella L,De Bellis G,Alifano P,Peano C

    更新日期:2017-05-12 00:00:00

  • A Comparative Genomic Analysis Provides Novel Insights Into the Ecological Success of the Monophasic Salmonella Serovar 4,[5],12:i:.

    abstract::Over the past decades, Salmonella 4,[5],12:i:- has rapidly emerged and it is isolated with high frequency in the swine food chain. Although many studies have documented the epidemiological success of this serovar, few investigations have tried to explain this phenomenon from a genetic perspective. Here a comparative w...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00715

    authors: Mastrorilli E,Pietrucci D,Barco L,Ammendola S,Petrin S,Longo A,Mantovani C,Battistoni A,Ricci A,Desideri A,Losasso C

    更新日期:2018-04-17 00:00:00

  • Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review.

    abstract::Aspergillus exists commonly in many crops and any process of crop growth, harvest, storage, and processing can be polluted by this fungus. Once it forms a biofilm, Aspergillus can produce many toxins, such as aflatoxin B1 (AFB1), ochratoxin, zearalenone, fumonisin, and patulin. Among these toxins, AFB1 possesses the h...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.00408

    authors: Wang Q,Yang Q,Wu W

    更新日期:2020-03-27 00:00:00

  • Efficacy of Live-Attenuated H9N2 Influenza Vaccine Candidates Containing NS1 Truncations against H9N2 Avian Influenza Viruses.

    abstract::H9N2 avian influenza virus is a zoonotic agent with a broad host range that can contribute genetic information to H5 or H7N9 subtype viruses, which are significant threats to both humans and birds. Thus, there is a great need for a vaccine to control H9N2 avian influenza. Three mutant viruses of an H9N2 virus A/chicke...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01086

    authors: Chen S,Zhu Y,Yang D,Yang Y,Shi S,Qin T,Peng D,Liu X

    更新日期:2017-06-14 00:00:00

  • Endophytic Bacterial Community Structure and Function of Herbaceous Plants From Petroleum Hydrocarbon Contaminated and Non-contaminated Sites.

    abstract::Bacterial endophytes (BEs) are non-pathogenic residents of healthy plant tissues that can confer benefits to plants. Many Bacterial endophytes have been shown to contribute to plant growth and health, alleviation of plant stress and to in-planta contaminant-degradation. This study examined the endophytic bacterial com...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01926

    authors: Lumactud R,Fulthorpe RR

    更新日期:2018-08-23 00:00:00

  • SIV replication in human cells.

    abstract::Current human immunodeficiency virus type 1 pandemic is believed to originate from cross-species transmission of simian immunodeficiency virus (SIV) into human population. Such cross-species transmission, however, is not efficient in general, because viral replication is modulated by host cell factors, with the specie...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00162

    authors: Sakuma R,Takeuchi H

    更新日期:2012-04-27 00:00:00

  • In vivo Activity of Copper(II), Manganese(II), and Silver(I) 1,10-Phenanthroline Chelates Against Candida haemulonii Using the Galleria mellonella Model.

    abstract::Candida haemulonii is an emerging opportunistic pathogen resistant to most antifungal drugs currently used in clinical arena. Metal complexes containing 1,10-phenanthroline (phen) chelating ligands have well-established anti-Candida activity against different medically relevant species. This study utilized larvae of G...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00470

    authors: Gandra RM,McCarron P,Viganor L,Fernandes MF,Kavanagh K,McCann M,Branquinha MH,Santos ALS,Howe O,Devereux M

    更新日期:2020-03-24 00:00:00

  • Population Genomics of Mycobacterium leprae Reveals a New Genotype in Madagascar and the Comoros.

    abstract::Human settlement of Madagascar traces back to the beginning of the first millennium with the arrival of Austronesians from Southeast Asia, followed by migrations from Africa and the Middle East. Remains of these different cultural, genetic, and linguistic legacies are still present in Madagascar and other islands of t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00711

    authors: Avanzi C,Lécorché E,Rakotomalala FA,Benjak A,Rapelanoro Rabenja F,Ramarozatovo LS,Cauchoix B,Rakoto-Andrianarivelo M,Tió-Coma M,Leal-Calvo T,Busso P,Boy-Röttger S,Chauffour A,Rasamoelina T,Andrianarison A,Sendrasoa F,Spen

    更新日期:2020-05-11 00:00:00

  • Unraveling the Complexity of Soil Microbiomes in a Large-Scale Study Subjected to Different Agricultural Management in Styria.

    abstract::Healthy soil microbiomes are crucial for achieving high productivity in combination with crop quality, but our understanding of microbial diversity is still limited. In a large-scale study including 116 composite samples from vineyards, orchards and other crops from all over Styria (south-east Austria), agricultural m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01052

    authors: Köberl M,Wagner P,Müller H,Matzer R,Unterfrauner H,Cernava T,Berg G

    更新日期:2020-05-25 00:00:00

  • A Single-Nucleotide Deletion in the Transcription Factor Gene bcsmr1 Causes Sclerotial-Melanogenesis Deficiency in Botrytis cinerea.

    abstract::Botrytis cinerea is an important plant pathogenic fungus with a wide range of host. It usually produces black-colored sclerotia (BS) due to deposition of 1,8-dihydroxynaphthalene melanin in sclerotial melanogenesis. Our previous study (Zhou et al., 2018) reported six B. cinerea isolates producing orange-colored sclero...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02492

    authors: Zhou Y,Yang L,Wu M,Chen W,Li G,Zhang J

    更新日期:2017-12-12 00:00:00

  • Antibiotic Resistance Patterns of Pseudomonas spp. Isolated from the River Danube.

    abstract::Spread and persistence of antibiotic resistance pose a severe threat to human health, yet there is still lack of knowledge about reservoirs of antibiotic resistant bacteria in the environment. We took the opportunity of the Joint Danube Survey 3 (JDS3), the world's biggest river research expedition of its kind in 2013...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00586

    authors: Kittinger C,Lipp M,Baumert R,Folli B,Koraimann G,Toplitsch D,Liebmann A,Grisold AJ,Farnleitner AH,Kirschner A,Zarfel G

    更新日期:2016-05-03 00:00:00

  • Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer.

    abstract::Resistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin resistance. Curren...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01141

    authors: Dortet L,Bonnin RA,Le Hello S,Fabre L,Bonnet R,Kostrzewa M,Filloux A,Larrouy-Maumus G

    更新日期:2020-06-03 00:00:00

  • Characterization of a Novel Tanay Virus Isolated From Anopheles sinensis Mosquitoes in Yunnan, China.

    abstract::Globally, mosquitoes are known to be competent vectors to various arboviruses that cause serious and debilitating diseases to humans and animals. Conversely, mosquitoes harbor a wide array of insect specific viruses (ISVs) that are generally neglected. Extensive characterization of these ISVs is important in understan...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01963

    authors: Zhao L,Mwaliko C,Atoni E,Wang Y,Zhang Y,Zhan J,Hu X,Xia H,Yuan Z

    更新日期:2019-08-22 00:00:00

  • The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains.

    abstract::In this study the efficacy of two different methods for extracting lipopeptides produced by five Bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. High performance thin layer chromatography (HPTLC) was also used for the simultane...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00925

    authors: Dimkić I,Stanković S,Nišavić M,Petković M,Ristivojević P,Fira D,Berić T

    更新日期:2017-05-23 00:00:00

  • Characterization of a Fusarium graminearum Salicylate Hydroxylase.

    abstract::Salicylic acid (SA) plays an important role in regulating plant defense responses against pathogens. However, pathogens have evolved ways to manipulate plant SA-mediated defense signaling. Fusarium graminearum causes Fusarium head blight (FHB) and reduces crop yields and quality by producing various mycotoxins. In thi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03219

    authors: Hao G,Naumann TA,Vaughan MM,McCormick S,Usgaard T,Kelly A,Ward TJ

    更新日期:2019-01-08 00:00:00

  • Chlamydia Persistence: A Survival Strategy to Evade Antimicrobial Effects in-vitro and in-vivo.

    abstract::The Chlamydiaceae comprise a group of highly adapted bacterial pathogens sharing a unique intracellular lifestyle. Three Chlamydia species are pathogenic to humans: Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci. C. trachomatis is the leading bacterial cause of sexually-transmitted infections and ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.03101

    authors: Panzetta ME,Valdivia RH,Saka HA

    更新日期:2018-12-12 00:00:00

  • Update on Pyrin Functions and Mechanisms of Familial Mediterranean Fever.

    abstract::Mutations in the MEFV gene, which encodes the protein named pyrin (also called marenostrin or TRIM20), are associated with the autoinflammatory disease familial Mediterranean fever (FMF). Recent genetic and immunologic studies uncovered novel functions of pyrin and raised several new questions in relation to FMF patho...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.00456

    authors: Manukyan G,Aminov R

    更新日期:2016-03-31 00:00:00

  • Iron to Gas: Versatile Multiport Flow-Column Revealed Extremely High Corrosion Potential by Methanogen-Induced Microbiologically Influenced Corrosion (Mi-MIC).

    abstract::Currently, sulfate-reducing bacteria (SRB) is regarded as the main culprit of microbiologically influenced corrosion (MIC), mainly due to the low reported corrosion rates of other microorganisms. For example, the highest reported corrosion rate for methanogens is 0.065 mm/yr. However, by investigating methanogen-induc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00527

    authors: An BA,Kleinbub S,Ozcan O,Koerdt A

    更新日期:2020-03-31 00:00:00

  • Urea Amendment Decreases Microbial Diversity and Selects for Specific Nitrifying Strains in Eight Contrasting Agricultural Soils.

    abstract::Application of nitrogen (N) fertilizers, predominantly as urea, is a major source of reactive N in the environment, with wide ranging effects including increased greenhouse gas accumulation in the atmosphere and aquatic eutrophication. The soil microbial community is the principal driver of soil N cycling; thus, impro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00634

    authors: Staley C,Breuillin-Sessoms F,Wang P,Kaiser T,Venterea RT,Sadowsky MJ

    更新日期:2018-04-04 00:00:00

  • Microbial Small Talk: Volatiles in Fungal-Bacterial Interactions.

    abstract::There is increasing evidence that volatile organic compounds (VOCs) play an important role in the interactions between fungi and bacteria, two major groups of soil inhabiting microorganisms. Yet, most of the research has been focused on effects of bacterial volatiles on suppression of plant pathogenic fungi whereas li...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01495

    authors: Schmidt R,Etalo DW,de Jager V,Gerards S,Zweers H,de Boer W,Garbeva P

    更新日期:2016-01-05 00:00:00

  • Antibacterial Action of Jineol Isolated from Scolopendra subspinipes mutilans against Selected Foodborne Pathogens.

    abstract::This study was undertaken to assess the antibacterial potential of 3,8-dihydroxyquinoline (jineol) isolated from Scolopendra subspinipes mutilans against selected foodborne pathogens Escherichia coli O157:H7 and Staphylococcus aureus KCTC-1621. Jineol at the tested concentration (50 μL; corresponding to 250 μg/disk) e...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00552

    authors: Bajpai VK,Shukla S,Paek WK,Lim J,Kumar P,Na M

    更新日期:2017-03-28 00:00:00

  • Insights Into the Evolution of Staphylococcus aureus Daptomycin Resistance From an in vitro Bioreactor Model.

    abstract::The extensive use of daptomycin for treating complex methicillin-resistant Staphylococcus aureus infections has led to the emergence of daptomycin-resistant strains. Although genomic studies have identified mutations associated with daptomycin resistance, they have not necessarily provided insight into the evolution a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00345

    authors: Lasek-Nesselquist E,Lu J,Schneider R,Ma Z,Russo V,Mishra S,Pai MP,Pata JD,McDonough KA,Malik M

    更新日期:2019-02-28 00:00:00

  • Benthic microbial communities of coastal terrestrial and ice shelf Antarctic meltwater ponds.

    abstract::The numerous perennial meltwater ponds distributed throughout Antarctica represent diverse and productive ecosystems central to the ecological functioning of the surrounding ultra oligotrophic environment. The dominant taxa in the pond benthic communities have been well described however, little is known regarding the...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00485

    authors: Archer SD,McDonald IR,Herbold CW,Lee CK,Cary CS

    更新日期:2015-05-27 00:00:00

  • Inactivation Effect of Violet and Blue Light on ESKAPE Pathogens and Closely Related Non-pathogenic Bacterial Species - A Promising Tool Against Antibiotic-Sensitive and Antibiotic-Resistant Microorganisms.

    abstract::Due to the globally observed increase in antibiotic resistance of bacterial pathogens and the simultaneous decline in new antibiotic developments, the need for alternative inactivation approaches is growing. This is especially true for the treatment of infections with the problematic ESKAPE pathogens, which include En...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.612367

    authors: Hoenes K,Bauer R,Meurle T,Spellerberg B,Hessling M

    更新日期:2021-01-13 00:00:00

  • Food-bacteria interplay: pathometabolism of emetic Bacillus cereus.

    abstract::Bacillus cereus is a Gram-positive endospore forming bacterium known for its wide spectrum of phenotypic traits, enabling it to occupy diverse ecological niches. Although the population structure of B. cereus is highly dynamic and rather panmictic, production of the emetic B. cereus toxin cereulide is restricted to st...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00704

    authors: Ehling-Schulz M,Frenzel E,Gohar M

    更新日期:2015-07-14 00:00:00

  • Corrigendum: Foam Cells Control Mycobacterium tuberculosis Infection.

    abstract::[This corrects the article DOI: 10.3389/fmicb.2020.01394.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2020.594142

    authors: Agarwal P,Combes TW,Shojaee-Moradie F,Fielding B,Gordon S,Mizrahi V,Martinez FO

    更新日期:2020-10-27 00:00:00