Confocal Laser Scanning Microscopy for Analysis of Pseudomonas aeruginosa Biofilm Architecture and Matrix Localization.

Abstract:

:Most microbes can produce surface-associated or suspended aggregates called biofilms, which are encased within a biopolymer-rich matrix. The biofilm matrix provides structural integrity to the aggregates and shields the resident cells against environmental stressors, including antibiotic treatment. Microscopy permits examination of biofilm structure in relation to the spatial localization of important biofilm matrix components. This review highlights microscopic approaches to investigate bacterial biofilm assembly, matrix composition, and localization using Pseudomonas aeruginosa as a model organism. Initial microscopic investigations provided information about the role key matrix components play in elaborating biofilm aggregate structures. Additionally, staining of matrix components using specific labels revealed distinct positioning of matrix components within the aggregates relative to the resident cells. In some cases, it was found that individual matrix components co-localize within aggregates. The methodologies for studying the biofilm matrix are continuing to develop as our studies reveal novel aspects of its composition and function. We additionally describe some outstanding questions and how microscopy might be used to identify the functional aspects of biofilm matrix components.

journal_name

Front Microbiol

authors

Reichhardt C,Parsek MR

doi

10.3389/fmicb.2019.00677

subject

Has Abstract

pub_date

2019-04-02 00:00:00

pages

677

issn

1664-302X

journal_volume

10

pub_type

杂志文章,评审
  • Animal Models for Tuberculosis in Translational and Precision Medicine.

    abstract::Tuberculosis (TB) is a health threat to the global population. Anti-TB drugs and vaccines are key approaches for TB prevention and control. TB animal models are basic tools for developing biomarkers of diagnosis, drugs for therapy, vaccines for prevention and researching pathogenic mechanisms for identification of tar...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.00717

    authors: Zhan L,Tang J,Sun M,Qin C

    更新日期:2017-05-04 00:00:00

  • Potential Risk Areas of Aedes albopictus in South-Eastern Iran: A Vector of Dengue Fever, Zika, and Chikungunya.

    abstract::The possibility of the rapid and global spread of Zika, chikungunya, yellow fever, and dengue fever by Aedes albopictus is well documented and may be facilitated by changes in climate. To avert and manage health risks, climatic and topographic information can be used to model and forecast which areas may be most prone...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01660

    authors: Nejati J,Bueno-Marí R,Collantes F,Hanafi-Bojd AA,Vatandoost H,Charrahy Z,Tabatabaei SM,Yaghoobi-Ershadi MR,Hasanzehi A,Shirzadi MR,Moosa-Kazemi SH,Sedaghat MM

    更新日期:2017-09-05 00:00:00

  • Bacterial Composition and Diversity in Breast Milk Samples from Mothers Living in Taiwan and Mainland China.

    abstract::Human breast milk is widely recognized as the best source of nutrients for healthy growth and development of infants; it contains a diverse microbiota. Here, we characterized the diversity of the microbiota in the breast milk of East Asian women and assessed whether delivery mode influenced the microbiota in the milk ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00965

    authors: Li SW,Watanabe K,Hsu CC,Chao SH,Yang ZH,Lin YJ,Chen CC,Cao YM,Huang HC,Chang CH,Tsai YC

    更新日期:2017-05-30 00:00:00

  • Bacteriophages and Bacterial Plant Diseases.

    abstract::Losses in crop yields due to disease need to be reduced in order to meet increasing global food demands associated with growth in the human population. There is a well-recognized need to develop new environmentally friendly control strategies to combat bacterial crop disease. Current control measures involving the use...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.00034

    authors: Buttimer C,McAuliffe O,Ross RP,Hill C,O'Mahony J,Coffey A

    更新日期:2017-01-20 00:00:00

  • Rapid Sequencing of Multiple RNA Viruses in Their Native Form.

    abstract::Long-read nanopore sequencing by a MinION device offers the unique possibility to directly sequence native RNA. We combined an enzymatic poly-A tailing reaction with the native RNA sequencing to (i) sequence complex population of single-stranded (ss)RNA viruses in parallel, (ii) detect genome, subgenomic mRNA/mRNA sim...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00260

    authors: Wongsurawat T,Jenjaroenpun P,Taylor MK,Lee J,Tolardo AL,Parvathareddy J,Kandel S,Wadley TD,Kaewnapan B,Athipanyasilp N,Skidmore A,Chung D,Chaimayo C,Whitt M,Kantakamalakul W,Sutthent R,Horthongkham N,Ussery DW,Jonsson

    更新日期:2019-02-25 00:00:00

  • Who possesses drug resistance genes in the aquatic environment?: sulfamethoxazole (SMX) resistance genes among the bacterial community in water environment of Metro-Manila, Philippines.

    abstract::Recent evidence has shown that antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are ubiquitous in natural environments, including sites considered pristine. To understand the origin of ARGs and their dynamics, we must first define their actual presence in the natural bacterial assemblage. Her...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00102

    authors: Suzuki S,Ogo M,Miller TW,Shimizu A,Takada H,Siringan MA

    更新日期:2013-04-30 00:00:00

  • Respiratory Commensal Bacteria Corynebacterium pseudodiphtheriticum Improves Resistance of Infant Mice to Respiratory Syncytial Virus and Streptococcus pneumoniae Superinfection.

    abstract::Corynebacterium pseudodiphtheriticum is a Gram-positive bacterium found as a member of the normal microbiota of the upper respiratory tract. It was suggested that C. pseudodiphtheriticum may be potentially used as a next-generation probiotic for nasal application, although no deep studies were performed in this regard...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01613

    authors: Kanmani P,Clua P,Vizoso-Pinto MG,Rodriguez C,Alvarez S,Melnikov V,Takahashi H,Kitazawa H,Villena J

    更新日期:2017-08-23 00:00:00

  • Autophosphorylation and Cross-Phosphorylation of Protein Kinases from the Crenarchaeon Sulfolobus islandicus.

    abstract::Protein phosphorylation, one of the most important post-translational modifications, regulates almost every cellular process. Although signal transduction by protein phosphorylation is extensively studied in Eukaryotes and Bacteria, the knowledge of this process in archaea is greatly lagging behind, especially for Ser...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02173

    authors: Huang Q,Zhong Q,Mayaka JBA,Ni J,Shen Y

    更新日期:2017-11-07 00:00:00

  • A Novel Strategy for Efficient Agaro-Oligosaccharide Production Based on the Enzymatic Degradation of Crude Agarose in Flammeovirga pacifica WPAGA1.

    abstract::To avoid conflict between biofuel and food resource production, marine macroalgae (main algal polysaccharides) have been suggested as potent feedstock for biofuel production. Flammeovirga pacifica WPAGA1, a typical marine polysaccharide-degrading bacterium, can utilize crude agarose as the sole carbon source. Transcri...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01231

    authors: Gao B,Li L,Wu H,Zhu D,Jin M,Qu W,Zeng R

    更新日期:2019-06-12 00:00:00

  • Salmonella Interaction with and Passage through the Intestinal Mucosa: Through the Lens of the Organism.

    abstract::Salmonella enterica serotypes are invasive enteric pathogens spread through fecal contamination of food and water sources, and represent a constant public health threat around the world. The symptoms associated with salmonellosis and typhoid disease are largely due to the host response to invading Salmonella, and to t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00088

    authors: Hallstrom K,McCormick BA

    更新日期:2011-04-29 00:00:00

  • Potential of Lactic Acid Bacteria Isolated From Different Forages as Silage Inoculants for Improving Fermentation Quality and Aerobic Stability.

    abstract::We aimed at isolating lactic acid bacteria (LAB) from different plant materials to study their crossed-fermentation capacity in silos and to find strains able to confer enhanced aerobic stability to silage. A total of 129 LAB isolates were obtained from lucerne (alfalfa), maize, sorghum, ryegrass, rice, barley, canola...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.586716

    authors: Puntillo M,Gaggiotti M,Oteiza JM,Binetti A,Massera A,Vinderola G

    更新日期:2020-12-08 00:00:00

  • Genomic Analysis of Carbapenem-Resistant Acinetobacter baumannii Isolates Belonging to Major Endemic Clones in South America.

    abstract::Carbapenem-resistant Acinetobacter baumannii (CRAB) are emerging worldwide. In South America, clinical isolates presenting such a phenotype usually do not belong to the globally distributed international clone 2 (IC2). The majority of these isolates are also resistant to multiple other antimicrobials and are often des...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.584603

    authors: Nodari CS,Cayô R,Streling AP,Lei F,Wille J,Almeida MS,de Paula AI,Pignatari ACC,Seifert H,Higgins PG,Gales AC

    更新日期:2020-11-30 00:00:00

  • First Insights Into Bacterial Gastrointestinal Tract Communities of the Eurasian Beaver (Castor fiber).

    abstract::The Eurasian or European beaver (Castor fiber) is the second-largest living rodent after the capybara. It is a semi-aquatic animal known for building dams and lodges. They strictly feed on lignocellulose-rich plants and correspondingly harbor cellulolytic microbial communities in their digestive tract. In this study, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01646

    authors: Pratama R,Schneider D,Böer T,Daniel R

    更新日期:2019-07-25 00:00:00

  • Technological Microbiology: Development and Applications.

    abstract::Over thousands of years, modernization could be predicted for the use of microorganisms in the production of foods and beverages. However, the current accelerated pace of new food production is due to the rapid incorporation of biotechnological techniques that allow the rapid identification of new molecules and microo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.00827

    authors: Vitorino LC,Bessa LA

    更新日期:2017-05-10 00:00:00

  • Growth and membrane fluidity of food-borne pathogen Listeria monocytogenes in the presence of weak acid preservatives and hydrochloric acid.

    abstract::This study addresses a major issue in microbial food safety, the elucidation of correlations between acid stress and changes in membrane fluidity of the pathogen Listeria monocytogenes. In order to assess the possible role that membrane fluidity changes play in L. monocytogenes tolerance to antimicrobial acids (acetic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00152

    authors: Diakogiannis I,Berberi A,Siapi E,Arkoudi-Vafea A,Giannopoulou L,Mastronicolis SK

    更新日期:2013-06-14 00:00:00

  • Vibrio and Bacterial Communities Across a Pollution Gradient in the Bay of Bengal: Unraveling Their Biogeochemical Drivers.

    abstract::The highly populated coasts of the Bay of Bengal are particularly vulnerable to water-borne diseases, pollution and climatic extremes. The environmental factors behind bacterial community composition and Vibrio distribution were investigated in an estuarine system of a cholera-endemic region in the coastline of Bangla...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00594

    authors: Kopprio GA,Neogi SB,Rashid H,Alonso C,Yamasaki S,Koch BP,Gärdes A,Lara RJ

    更新日期:2020-04-15 00:00:00

  • Deletion of the Small RNA Chaperone Protein Hfq down Regulates Genes Related to Virulence and Confers Protection against Wild-Type Brucella Challenge in Mice.

    abstract::Brucellosis is one of the most common zoonotic epidemics worldwide. Brucella, the etiological pathogen of brucellosis, has unique virulence characteristics, including the ability to survive within the host cell. Hfq is a bacterial chaperone protein that is involved in the survival of the pathogen under stress conditio...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01570

    authors: Lei S,Zhong Z,Ke Y,Yang M,Xu X,Ren H,An C,Yuan J,Yu J,Xu J,Qiu Y,Shi Y,Wang Y,Peng G,Chen Z

    更新日期:2016-01-20 00:00:00

  • The Atypical Guanylate Kinase MoGuk2 Plays Important Roles in Asexual/Sexual Development, Conidial Septation, and Pathogenicity in the Rice Blast Fungus.

    abstract::Guanylate kinases (GKs), which convert guanosine monophosphate into guanosine diphosphate (GDP), are important for growth and mannose outer chain elongation of cell wall N-linked glycoproteins in yeast. Here, we identified the ortholog of Saccharomyces cerevisiae GK Guk1, named MoGuk1 and a novel family of fungal GKs ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02467

    authors: Cai X,Zhang X,Li X,Liu M,Liu X,Wang X,Zhang H,Zheng X,Zhang Z

    更新日期:2017-12-11 00:00:00

  • Cytotoxic effects of oosporein isolated from endophytic fungus Cochliobolus kusanoi.

    abstract::In the present study, oosporein, a fungal toxic secondary metabolite known to be a toxic agent causing chronic disorders in animals, was isolated from fungus Cochliobolus kusanoi of Nerium oleander L. Toxic effects of oosporein and the possible mechanisms of cytotoxicity as well as the role of oxidative stress in cyto...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00870

    authors: Ramesha A,Venkataramana M,Nirmaladevi D,Gupta VK,Chandranayaka S,Srinivas C

    更新日期:2015-09-01 00:00:00

  • Microbial Small Talk: Volatiles in Fungal-Bacterial Interactions.

    abstract::There is increasing evidence that volatile organic compounds (VOCs) play an important role in the interactions between fungi and bacteria, two major groups of soil inhabiting microorganisms. Yet, most of the research has been focused on effects of bacterial volatiles on suppression of plant pathogenic fungi whereas li...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01495

    authors: Schmidt R,Etalo DW,de Jager V,Gerards S,Zweers H,de Boer W,Garbeva P

    更新日期:2016-01-05 00:00:00

  • The Complete Sequence and Comparative Analysis of a Multidrug-Resistance and Virulence Multireplicon IncFII Plasmid pEC302/04 from an Extraintestinal Pathogenic Escherichia coli EC302/04 Indicate Extensive Diversity of IncFII Plasmids.

    abstract::Extraintestinal pathogenic Escherichia coli (ExPEC) that causes extraintestinal infections often harbor plasmids encoding fitness traits such as resistance and virulence determinants that are of clinical importance. We determined the complete nucleotide sequence of plasmid pEC302/04 from a multidrug-resistant E. coli ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01547

    authors: Ho WS,Yap KP,Yeo CC,Rajasekaram G,Thong KL

    更新日期:2016-01-11 00:00:00

  • Ecology and management of grapevine leafroll disease.

    abstract::Grapevine leafroll disease (GLD) is caused by a complex of vector-borne virus species in the family Closteroviridae. GLD is present in all grape-growing regions of the world, primarily affecting wine grape varieties. The disease has emerged in the last two decades as one of the major factors affecting grape fruit qual...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00094

    authors: Almeida RP,Daane KM,Bell VA,Blaisdell GK,Cooper ML,Herrbach E,Pietersen G

    更新日期:2013-04-24 00:00:00

  • Bio-Decolorization of Synthetic Dyes by a Halophilic Bacterium Salinivibrio sp.

    abstract::Synthetic dyes, extensively used in various industries, act as pollutants in the aquatic environment, and pose a significant threat to living beings. In the present study, we assessed the potential of a halophilic bacterium Salinivibrio kushneri HTSP isolated from a saltpan for decolorization and bioremediation of syn...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.594011

    authors: John J,Dineshram R,Hemalatha KR,Dhassiah MP,Gopal D,Kumar A

    更新日期:2020-12-21 00:00:00

  • The Antifungal Plant Defensin HsAFP1 from Heuchera sanguinea Induces Apoptosis in Candida albicans.

    abstract::Plant defensins are active against plant and human pathogenic fungi (such as Candida albicans) and baker's yeast. However, they are non-toxic to human cells, providing a possible source for treatment of fungal infections. In this study, we characterized the mode of action of the antifungal plant defensin HsAFP1 from c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00047

    authors: Aerts AM,Bammens L,Govaert G,Carmona-Gutierrez D,Madeo F,Cammue BP,Thevissen K

    更新日期:2011-03-16 00:00:00

  • Nutrient Dependent Cross-Kingdom Interactions: Fungi and Bacteria From an Oligotrophic Desert Oasis.

    abstract::Microbial interactions play a key role in ecosystem functioning, with nutrient availability as an important determinant. Although phylogenetically distant bacteria and fungi commonly co-occur in nature, information on their cross-kingdom interactions under unstable, extreme environments remains poor. Hence, the aims o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01755

    authors: Velez P,Espinosa-Asuar L,Figueroa M,Gasca-Pineda J,Aguirre-von-Wobeser E,Eguiarte LE,Hernandez-Monroy A,Souza V

    更新日期:2018-08-06 00:00:00

  • Coronaviruses in the Sea.

    abstract::Interest in coronaviruses because of the 2019 novel coronavirus (SARS-CoV-2) pandemic has generated concern about their occurrence and persistence in aquatic habitats. Coronaviruses are not quantitatively significant constituents of marine virioplankton. Members of the Nidovirales (to which human coronaviruses belong)...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01795

    authors: Mordecai GJ,Hewson I

    更新日期:2020-07-24 00:00:00

  • The Respiratory Microbiome in Cystic Fibrosis: Compartment Patterns and Clinical Relationships in Early Stage Disease.

    abstract::We compared the bacterial microbiomes lodged in the bronchial tree, oropharynx and nose of patients with early stage cystic fibrosis (CF) not using chronic antibiotics, determining their relationships with lung function and exacerbation frequency. CF patients were enrolled in a cohort study during stability and were c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01463

    authors: Garcia-Nuñez M,Garcia-Gonzalez M,Pomares X,Montón C,Millares L,Quero S,Prina E,Asensio O,Bosque M,Capilla S,Cuevas O,Monsó E

    更新日期:2020-06-30 00:00:00

  • The Potential Role of Gut Mycobiome in Irritable Bowel Syndrome.

    abstract::The human gut is inhabited by diverse microorganisms that play crucial roles in health and disease. Gut microbiota dysbiosis is increasingly considered as a vital factor in the etiopathogenesis of irritable bowel syndrome (IBS), which is a common functional gastrointestinal disorder with a high incidence all over the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.01894

    authors: Gu Y,Zhou G,Qin X,Huang S,Wang B,Cao H

    更新日期:2019-08-21 00:00:00

  • Dose Assessment of Cefquinome by Pharmacokinetic/Pharmacodynamic Modeling in Mouse Model of Staphylococcus aureus Mastitis.

    abstract::This work aimed to characterize the mammary gland pharmacokinetics of cefquinome after an intramammary administration and integrate pharmacokinetic/pharmacodynamic model. The pharmacokinetic profiles of cefquinome in gland tissue were measured using high performance liquid chromatograph. Therapeutic regimens covered v...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01595

    authors: Yu Y,Zhou YF,Li X,Chen MR,Qiao GL,Sun J,Liao XP,Liu YH

    更新日期:2016-10-07 00:00:00

  • Regulatory RNAs in the Less Studied Streptococcal Species: From Nomenclature to Identification.

    abstract::Streptococcal species are Gram-positive bacteria involved in severe and invasive diseases in humans and animals. Although, this group includes different pathogenic species involved in life-threatening infections for humans, it also includes beneficial species, such as Streptococcus thermophilus, which is used in yogur...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01161

    authors: Zorgani MA,Quentin R,Lartigue MF

    更新日期:2016-07-26 00:00:00