The Antifungal Plant Defensin HsAFP1 from Heuchera sanguinea Induces Apoptosis in Candida albicans.

Abstract:

:Plant defensins are active against plant and human pathogenic fungi (such as Candida albicans) and baker's yeast. However, they are non-toxic to human cells, providing a possible source for treatment of fungal infections. In this study, we characterized the mode of action of the antifungal plant defensin HsAFP1 from coral bells by screening the Saccharomyces cerevisiae deletion mutant library for mutants with altered HsAFP1 sensitivity and verified the obtained genetic data by biochemical assays in S. cerevisiae and C. albicans. We identified 84 genes, which when deleted conferred at least fourfold hypersensitivity or resistance to HsAFP1. A considerable part of these genes were found to be implicated in mitochondrial functionality. In line, sodium azide, which blocks the respiratory electron transport chain, antagonized HsAFP1 antifungal activity, suggesting that a functional respiratory chain is indispensable for HsAFP1 antifungal action. Since mitochondria are the main source of cellular reactive oxygen species (ROS), we investigated the ROS-inducing nature of HsAFP1. We showed that HsAFP1 treatment of C. albicans resulted in ROS accumulation. As ROS accumulation is one of the phenotypic markers of apoptosis in yeast, we could further demonstrate that HsAFP1 induced apoptosis in C. albicans. These data provide novel mechanistic insights in the mode of action of a plant defensin.

journal_name

Front Microbiol

authors

Aerts AM,Bammens L,Govaert G,Carmona-Gutierrez D,Madeo F,Cammue BP,Thevissen K

doi

10.3389/fmicb.2011.00047

subject

Has Abstract

pub_date

2011-03-16 00:00:00

pages

47

issn

1664-302X

journal_volume

2

pub_type

杂志文章
  • Semi-Quantification of Total Campylobacter and Salmonella During Egg Incubations Using a Combination of 16S rDNA and Specific Pathogen Primers for qPCR.

    abstract::Rapid molecular techniques that evaluate eggs for the presence of foodborne pathogens is an essential component to poultry food safety monitoring. Interestingly, it is not just table eggs that contribute to outbreaks of foodborne disease. Broiler layer production actively contributes to sustaining of foodborne pathoge...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02454

    authors: Rothrock MJ Jr,Feye KM,Kim SA,Park SH,Locatelli A,Hiett KL,Gamble J,Sellers H,Ricke SC

    更新日期:2018-11-02 00:00:00

  • Estimation of Candida albicans ABC Transporter Behavior in Real-Time via Fluorescence.

    abstract::We present a fluorometric method for determining ABC transporter activity in the pathogenic fungus C. albicans during different growth phases and in response to glucose. The carbocyanine dye diS-C3(3) was previously used to monitor plasma membrane potentials and test the influence of surface-active compounds in membra...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01382

    authors: Szczepaniak J,Łukaszewicz M,Krasowska A

    更新日期:2015-12-09 00:00:00

  • Saccharomyces cerevisiae - Insects Association: Impacts, Biogeography, and Extent.

    abstract::Over the last few years, an increasing number of studies have reported the existence of an association between the budding yeast Saccharomyces cerevisiae and insects. The discovery of this relationship has called into question the hypothesis that S. cerevisiae is unable to survive in nature and that the presence of S....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01629

    authors: Meriggi N,Di Paola M,Cavalieri D,Stefanini I

    更新日期:2020-07-14 00:00:00

  • Potent Neutralization Ability of a Human Monoclonal Antibody Against Serotype 1 Dengue Virus.

    abstract::The incidence of dengue virus (DENV) infections has been escalating in tropical and subtropical countries, but there are still no effective therapeutic options. In the present study, a DENV-1-specific human monoclonal antibody (HMAb), 1G5, isolated from single plasma cells obtained from the peripheral blood mononuclea...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01214

    authors: Lu J,Wang R,Xia B,Yu Y,Zhou X,Yang Z,Huang P

    更新日期:2018-06-06 00:00:00

  • The mgtCBR mRNA Leader Secures Growth of Salmonella in Both Host and Non-host Environments.

    abstract::Upon intracellular cues, bacterial mRNA leaders often form secondary structures that determine expression of a downstream protein-coding region(s), thereby providing bacteria with a mechanism to control the amounts of necessary proteins in the right locales. Here we describe a polycistronic mRNA leader that secures ba...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02831

    authors: Park M,Kim H,Nam D,Kweon DH,Shin D

    更新日期:2019-12-06 00:00:00

  • Interplay Between the Phenotype and Genotype, and Efflux Pumps in Drug-Resistant Strains of Riemerella anatipestifer.

    abstract::The number of multidrug-resistant strains of Riemerella anatipestifer continues to increase, and new strategies for the treatment of associated infections are necessary. Recently, numerous studies have shown that efflux pumps (EPs) play key roles in universal bacterial mechanisms that contribute to antibiotic resistan...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02136

    authors: Chen Q,Gong X,Zheng F,Ji G,Li S,Stipkovits L,Szathmary S,Liu Y

    更新日期:2018-10-01 00:00:00

  • High Iron-Sequestrating Bifidobacteria Inhibit Enteropathogen Growth and Adhesion to Intestinal Epithelial Cells In vitro.

    abstract::The gut microbiota plays an important role in host health, in particular by its barrier effect and competition with exogenous pathogenic bacteria. In the present study, the competition of Bifidobacterium pseudolongum PV8-2 (Bp PV8-2) and Bifidobacterium kashiwanohense PV20-2 (Bk PV20-2), isolated from anemic infant gu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01480

    authors: Vazquez-Gutierrez P,de Wouters T,Werder J,Chassard C,Lacroix C

    更新日期:2016-09-22 00:00:00

  • Interactions Between Thiamethoxam and Deformed Wing Virus Can Drastically Impair Flight Behavior of Honey Bees.

    abstract::Exposure to multiple stress factors is believed to contribute to honey bee colony decline. However, little is known about how co-exposure to stress factors can alter the survival and behavior of free-living honey bees in colony conditions. We therefore studied the potential interaction between a neonicotinoid pesticid...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00766

    authors: Coulon M,Dalmon A,Di Prisco G,Prado A,Arban F,Dubois E,Ribière-Chabert M,Alaux C,Thiéry R,Le Conte Y

    更新日期:2020-04-30 00:00:00

  • Stuck in the Middle: Fibronectin-Binding Proteins in Gram-Positive Bacteria.

    abstract::Fibronectin is a multidomain glycoprotein found ubiquitously in human body fluids and extracellular matrices of a variety of cell types from all human tissues and organs, including intestinal epithelial cells. Fibronectin plays a major role in the regulation of cell migration, tissue repair, and cell adhesion. Importa...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01504

    authors: Hymes JP,Klaenhammer TR

    更新日期:2016-09-22 00:00:00

  • Protein Kinase Ime2 Is Required for Mycelial Growth, Conidiation, Osmoregulation, and Pathogenicity in Nematode-Trapping Fungus Arthrobotrys oligospora.

    abstract::Inducer of meiosis 2 (Ime2), a protein kinase that has been identified in diverse fungal species, functions in the regulation of various cellular processes, such as ascospore formation, pseudohyphal growth, and sexual reproduction. In this study, AoIme2, an ortholog of Saccharomyces cerevisiae Ime2, was characterized ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03065

    authors: Xie M,Bai N,Yang J,Jiang K,Zhou D,Zhao Y,Li D,Niu X,Zhang KQ,Yang J

    更新日期:2020-01-14 00:00:00

  • Genome-Wide Identification and Classification of Soybean C2H2 Zinc Finger Proteins and Their Expression Analysis in Legume-Rhizobium Symbiosis.

    abstract::Root nodule symbiosis (RNS) is one of the most productive and economical systems for nitrogen fixation, and previous studies have shown that several nodule-specific C2H2-zinc finger proteins (ZFPs) play important roles in symbiosis establishment and nodule function. However, C2H2-ZFPs are the most widespread ZFPs in e...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00126

    authors: Yuan S,Li X,Li R,Wang L,Zhang C,Chen L,Hao Q,Zhang X,Chen H,Shan Z,Yang Z,Chen S,Qiu D,Ke D,Zhou X

    更新日期:2018-02-06 00:00:00

  • The Strengths of Scanning Electron Microscopy in Deciphering SARS-CoV-2 Infectious Cycle.

    abstract::Electron microscopy is a powerful tool in the field of microbiology. It has played a key role in the rapid diagnosis of viruses in patient samples and has contributed significantly to the clarification of virus structure and function, helping to guide the public health response to emerging viral infections. In the pre...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.02014

    authors: Brahim Belhaouari D,Fontanini A,Baudoin JP,Haddad G,Le Bideau M,Bou Khalil JY,Raoult D,La Scola B

    更新日期:2020-08-19 00:00:00

  • Fungal ITS1 Deep-Sequencing Strategies to Reconstruct the Composition of a 26-Species Community and Evaluation of the Gut Mycobiota of Healthy Japanese Individuals.

    abstract::The study of mycobiota remains relatively unexplored due to the lack of sufficient available reference strains and databases compared to those of bacterial microbiome studies. Deep sequencing of Internal Transcribed Spacer (ITS) regions is the de facto standard for fungal diversity analysis. However, results are often...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00238

    authors: Motooka D,Fujimoto K,Tanaka R,Yaguchi T,Gotoh K,Maeda Y,Furuta Y,Kurakawa T,Goto N,Yasunaga T,Narazaki M,Kumanogoh A,Horii T,Iida T,Takeda K,Nakamura S

    更新日期:2017-02-15 00:00:00

  • Building of Pressure-Assisted Ultra-High Temperature System and Its Inactivation of Bacterial Spores.

    abstract::The pressure-assisted ultra-high temperature (PAUHT) system was built by using soybean oil as pressure-transmitting medium, and the multiple regression equation of soybean oil temperature change (ΔTP ) during pressurization as a function of initial temperature (Ti ) and set pressure (P) was developed: ΔTP = -13.45 + 0...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01275

    authors: Liang D,Zhang L,Wang X,Wang P,Liao X,Wu X,Chen F,Hu X

    更新日期:2019-06-10 00:00:00

  • Analysis of the Rumen Microbiome and Metabolome to Study the Effect of an Antimethanogenic Treatment Applied in Early Life of Kid Goats.

    abstract::This work aimed to gain insight into the transition from milk to solid feeding at weaning combining genomics and metabolomics on rumen contents from goat kids treated with a methanogenic inhibitor (bromochloromethane, BCM). Sixteen goats giving birth to two kids were used. Eight does were treated (D+) with BCM after g...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02227

    authors: Abecia L,Martínez-Fernandez G,Waddams K,Martín-García AI,Pinloche E,Creevey CJ,Denman SE,Newbold CJ,Yáñez-Ruiz DR

    更新日期:2018-10-09 00:00:00

  • Niche Filtering of Bacteria in Soil and Rock Habitats of the Colorado Plateau Desert, Utah, USA.

    abstract::A common feature of microbial colonization in deserts is biological soil crusts (BSCs), and these comprise a complex community dominated by Cyanobacteria. Rock substrates, particularly sandstone, are also colonized by microbial communities. These are separated by bare sandy soil that also supports microbial colonizati...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01489

    authors: Lee KC,Archer SD,Boyle RH,Lacap-Bugler DC,Belnap J,Pointing SB

    更新日期:2016-09-26 00:00:00

  • Lipopolysaccharide Downregulates CD163 Expression to Inhibit PRRSV Infection via TLR4-NF-κB Pathway.

    abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) has been recognized to induce proinflammatory cytokine production and modulate the host interferon (IFN) system. Proinflammatory cytokines and type I IFNs contribute to the prevention of viral infection. Lipopolysaccharide (LPS), a specific agonist to Toll-li...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00501

    authors: Zhu Z,Zhang H,Zhang X,He S,Dong W,Wang X,Chen Y,Liu X,Guo C

    更新日期:2020-03-25 00:00:00

  • A Recombinant Subviral Particle-Based Vaccine Protects Magpie (Pica pica) Against West Nile Virus Infection.

    abstract::The mosquito-borne West Nile virus (WNV) is a highly neurovirulent Flavivirus currently representing an emergent zoonotic concern. WNV cycles in nature between mosquito vectors and birds that act as amplifier hosts and play an essential role in virus ecology, being, thus, WNV a threat to many species. Availability of ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01133

    authors: Jiménez de Oya N,Escribano-Romero E,Camacho MC,Blazquez AB,Martín-Acebes MA,Höfle U,Saiz JC

    更新日期:2019-06-05 00:00:00

  • Gene Turnover Contributes to the Evolutionary Adaptation of Acidithiobacillus caldus: Insights from Comparative Genomics.

    abstract::Acidithiobacillus caldus is an extremely acidophilic sulfur-oxidizer with specialized characteristics, such as tolerance to low pH and heavy metal resistance. To gain novel insights into its genetic complexity, we chosen six A. caldus strains for comparative survey. All strains analyzed in this study differ in geograp...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01960

    authors: Zhang X,Liu X,He Q,Dong W,Zhang X,Fan F,Peng D,Huang W,Yin H

    更新日期:2016-12-06 00:00:00

  • Engineered Bacteriophage T7 as a Potent Anticancer Agent in vivo.

    abstract::Oncolytic viruses (OVs) induce antitumor effect by both direct lysis of target cells and eliciting immunogenic response to the virus and ultimately to the target cells. These viruses are usually natural human pathogens. Bacteriophages are natural pathogens of bacteria that do not infect human and have greater advantag...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.491001

    authors: Hwang YJ,Myung H

    更新日期:2020-09-24 00:00:00

  • Dual Oxidase-Derived Reactive Oxygen Species Against Bacillus thuringiensis and Its Suppression by Eicosanoid Biosynthesis Inhibitors.

    abstract::Two entomopathogenic bacteria, Xenorhabdus and Photorhabdus, are known to be able to synthesize and secrete eicosanoid biosynthesis inhibitors (EIBs) that can enhance pathogenicity of Bacillus thuringiensis (Bt) against different target insects. Such enhancements can be explained by the suppression of immune responses...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00528

    authors: Sajjadian SM,Kim Y

    更新日期:2020-03-27 00:00:00

  • Description and Comparative Genomics of Macrococcus caseolyticus subsp. hominis subsp. nov., Macrococcus goetzii sp. nov., Macrococcus epidermidis sp. nov., and Macrococcus bohemicus sp. nov., Novel Macrococci From Human Clinical Material With Virulence P

    abstract::The genus Macrococcus is a close relative of the genus Staphylococcus. Whilst staphylococci are widespread as human pathogens, macrococci have not yet been reported from human clinical specimens. Here we investigated Gram-positive and catalase-positive cocci recovered from human clinical material and identified as Mac...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01178

    authors: Mašlaňová I,Wertheimer Z,Sedláček I,Švec P,Indráková A,Kovařovic V,Schumann P,Spröer C,Králová S,Šedo O,Krištofová L,Vrbovská V,Füzik T,Petráš P,Zdráhal Z,Ružičková V,Doškař J,Pantuček R

    更新日期:2018-06-13 00:00:00

  • Beyond a Ribosomal RNA Methyltransferase, the Wider Role of MraW in DNA Methylation, Motility and Colonization in Escherichia coli O157:H7.

    abstract::MraW is a 16S rRNA methyltransferase and plays a role in the fine-tuning of the ribosomal decoding center. It was recently found to contribute to the virulence of Staphylococcus aureus. In this study, we examined the function of MraW in Escherichia coli O157:H7 and found that the deletion of mraW led to decreased moti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02520

    authors: Xu X,Zhang H,Huang Y,Zhang Y,Wu C,Gao P,Teng Z,Luo X,Peng X,Wang X,Wang D,Pu J,Zhao H,Lu X,Lu S,Ye C,Dong Y,Lan R,Xu J

    更新日期:2019-11-13 00:00:00

  • Natural Killer Cell Functions during the Innate Immune Response to Pathogenic Streptococci.

    abstract::Dendritic cells (DCs) and NK cells play a crucial role in the first phase of host defense against infections. Group B Streptococcus (GBS) and Streptococcus suis are encapsulated streptococci causing severe systemic inflammation, leading to septicemia and meningitis. Yet, the involvement of NK cells in the innate immun...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01196

    authors: Lemire P,Galbas T,Thibodeau J,Segura M

    更新日期:2017-06-29 00:00:00

  • Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth.

    abstract::Giardiasis, currently considered a neglected disease, is caused by the intestinal protozoan parasite Giardia duodenalis and is widely spread in human as well as domestic and wild animals. The lack of appropriate medications and the spread of resistant parasite strains urgently call for the development of novel therape...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01453

    authors: Travers MA,Sow C,Zirah S,Deregnaucourt C,Chaouch S,Queiroz RM,Charneau S,Allain T,Florent I,Grellier P

    更新日期:2016-09-27 00:00:00

  • c-di-GMP Regulates Various Phenotypes and Insecticidal Activity of Gram-Positive Bacillus thuringiensis.

    abstract::C-di-GMP has been well investigated to play significant roles in the physiology of many Gram-negative bacteria. However, its effect on Gram-positive bacteria is less known. In order to more understand the c-di-GMP functions in Gram-positive bacteria, we have carried out a detailed study on the c-di-GMP-metabolizing en...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00045

    authors: Fu Y,Yu Z,Liu S,Chen B,Zhu L,Li Z,Chou SH,He J

    更新日期:2018-02-13 00:00:00

  • In vitro and in vivo Virulence Potential of the Emergent Species of the Acinetobacter baumannii (Ab) Group.

    abstract::The increased use of molecular identification methods and mass spectrometry has revealed that Acinetobacter spp. of the A. baumannii (Ab) group other than A. baumannii are increasingly being recovered from human samples and may pose a health challenge if neglected. In this study 76 isolates of 5 species within the Ab ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02429

    authors: Cosgaya C,Ratia C,Marí-Almirall M,Rubio L,Higgins PG,Seifert H,Roca I,Vila J

    更新日期:2019-10-24 00:00:00

  • Orf Virus 002 Protein Targets Ovine Protein S100A4 and Inhibits NF-κB Signaling.

    abstract::Orf virus (ORFV), a member of Parapoxvirus, has evolved various strategies to modulate the immune responses of host cells. The ORFV-encoded protein ORFV002, a regulator factor, has been found to inhibit the acetylation of NF-κB-p65 by blocking phosphorylation of NF-κB-p65 at Ser(276) and also to disrupt the binding of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01389

    authors: Chen D,Zheng Z,Xiao B,Li W,Long M,Chen H,Li M,Rock DL,Hao W,Luo S

    更新日期:2016-09-13 00:00:00

  • Identification of the Tolfenamic Acid Binding Pocket in PrbP from Liberibacter asiaticus.

    abstract::In Liberibacter asiaticus, PrbP is an important transcriptional accessory protein that was found to regulate gene expression through interactions with the RNA polymerase β-subunit and a specific sequence on the promoter region. It was found that inactivation of PrbP, using the inhibitor tolfenamic acid, resulted in a ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01591

    authors: Pan L,Gardner CL,Pagliai FA,Gonzalez CF,Lorca GL

    更新日期:2017-08-23 00:00:00

  • Comparative Genomics of DNA Recombination and Repair in Cyanobacteria: Biotechnological Implications.

    abstract::Cyanobacteria are fascinating photosynthetic prokaryotes that are regarded as the ancestors of the plant chloroplast; the purveyors of oxygen and biomass for the food chain; and promising cell factories for an environmentally friendly production of chemicals. In colonizing most waters and soils of our planet, cyanobac...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01809

    authors: Cassier-Chauvat C,Veaudor T,Chauvat F

    更新日期:2016-11-09 00:00:00