Abstract:
:Human cytomegalovirus-induced DNA polymerase and cellular DNA polymerase alpha were purified by successive chromatography on DEAE-cellulose, phosphocellulose, heparin agarose, and single-stranded DNA agarose columns. The purified virus-induced DNA polymerase was resolved to consist of two polypeptides corresponding to molecular weights of 140,000 and 58,000, as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Virus-induced DNA polymerase and cellular alpha polymerase were examined for their sensitivities to the triphosphates of 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)-5-methyluracil (FMAUTP), -5-iodocytosine (FIACTP), and -5-methylcytosine (FMACTP). The inhibitive effects of these triphosphates on the DNA polymerases were competitive with regard to the natural substrates; thus FMAUTP competes with dTTP, and FIACTP and FMACTP compete with dCTP. The inhibition constants (Ki) for FMAUTP, FIACTP, and FMACTP of virus-induced DNA polymerase are 0.06, 0.30, and 0.47 microM, respectively. Cellular DNA polymerase alpha is much less sensitive to these inhibitors, and its Ki values for FMAUTP, FIACTP, and FMACTP are 0.45, 3.10, and 2.90 microM, respectively. In addition, human cytomegalovirus-induced DNA polymerase, but not cellular DNA polymerase alpha, can utilize these analog triphosphates as alternate substrates for their corresponding natural deoxyribonucleoside triphosphates in in vitro DNA synthesis.
journal_name
J Viroljournal_title
Journal of virologyauthors
Mar EC,Chiou JF,Cheng YC,Huang ESdoi
10.1128/JVI.56.3.846-851.1985subject
Has Abstractpub_date
1985-12-01 00:00:00pages
846-51issue
3eissn
0022-538Xissn
1098-5514journal_volume
56pub_type
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pub_type: 杂志文章
doi:10.1128/JVI.42.1.131-134.1982
更新日期:1982-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.17.2.675-677.1976
更新日期:1976-02-01 00:00:00
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更新日期:2000-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.6.736-748.1971
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1979-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2015-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1579-1589.1993
更新日期:1993-03-01 00:00:00