Abstract：
:Surgical sutures represent the gold standard for wound closure, however, their main purpose is still limited to a mechanical function rather than playing a bioactive role. Since oxygen and pro-regenerative growth factors have been broadly described as key players for the healing process, in this study we evaluated the feasibility of generating photosynthetic sutures that, in addition to mechanical fixation, could locally and stably release oxygen and recombinant human growth factors (VEGF, PDGF-BB, or SDF-1α) at the wound site. Here, photosynthetic genetically modified microalgae were seeded in commercially available sutures and their distribution and proliferation capacity was evaluated. Additionally, the mechanical properties of seeded sutures were compared to unseeded controls that showed no significant differences. Oxygen production, as well as recombinant growth factor release was quantified in vitro over time, and confirmed that photosynthetic sutures are indeed a feasible approach for the local delivery of bioactive molecules. Finally, photosynthetic sutures were tested in order to evaluate their resistance to mechanical stress and freezing. Significant stability was observed in both conditions, and the feasibility of their use in the clinical practice was therefore confirmed. Our results suggest that photosynthetic gene therapy could be used to produce a new generation of bioactive sutures with improved healing capacities. STATEMENT OF SIGNIFICANCE: Disruption of the vascular network is intrinsic to trauma and surgery, and consequently, wound healing is characterized by diminished levels of blood perfusion. Among all the blood components, oxygen and pro-regenerative growth factors have been broadly described as key players for the healing process. Therefore, in this study we evaluated the feasibility of generating photosynthetic sutures that, in addition to mechanical fixation, could locally and stably release oxygen and recombinant human growth factors at the wound site. This novel concept has never been explored before for this type of material and represents the first attempt to create a new generation of bioactive sutures with improved regenerative capabilities.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Centeno-Cerdas C,Jarquín-Cordero M,Chávez MN,Hopfner U,Holmes C,Schmauss D,Machens HG,Nickelsen J,Egaña JTdoi
10.1016/j.actbio.2018.09.060subject
Has Abstractpub_date
2018-11-01 00:00:00pages
184-194eissn
1742-7061issn
1878-7568pii
S1742-7061(18)30589-0journal_volume
81pub_type
杂志文章abstract：:Bone loss around tooth extraction sites can occur, thus making future placement of dental implants difficult. Alveolar bone regeneration can be guided by the application of a nanofibrous bone graft coupled with osteoinductive proteins/peptides, following tooth loss or tooth extraction. In the present study, we demonst...
journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2019-02-01 00:00:00
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journal_title：Acta biomaterialia
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journal_title：Acta biomaterialia
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.11.029
更新日期：2011-04-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.05.016
更新日期：2016-09-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.07.034
更新日期：2010-01-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.07.047
更新日期：2019-09-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.03.035
更新日期：2016-08-01 00:00:00
abstract：:Magnesium (Mg) is a promising biomaterial for degradable implant applications that has been extensively studied in vitro and in vivo in recent years. In this study, we developed a procedure that allows an optimized and uniform in vitro assessment of the cytocompatibility of Mg-based materials while respecting the stan...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.06.005
更新日期：2015-09-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.03.023
更新日期：2015-07-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.06.028
更新日期：2020-09-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.03.003
更新日期：2018-04-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2018.04.010
更新日期：2018-06-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.11.032
更新日期：2021-02-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.03.003
更新日期：2010-08-01 00:00:00
abstract：:The application of biodegradable magnesium-based materials in the biomedical field is highly restricted by their low fatigue strength and high corrosion rate in biological environments. Herein, we treated the surface of a biocompatible magnesium alloy AZ31 by severe shot peening in order to evaluate the potential of s...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.11.032
更新日期：2018-01-15 00:00:00
abstract：:Bone replacement using synthetic and degradable materials is desirable in various clinical conditions. Most applied commercial materials are based on hydroxyapatite, which is not chemically degradable under physiological conditions. Here we report the effect of a long-term intramuscular implantation regime on the diss...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2011.05.022
更新日期：2011-09-01 00:00:00
abstract：:A biphasic layer with a Zn-containing beta-tricalcium phosphate (ZnTCP) phase and a fluoridated hydroxyapatite (FHA) phase on titanium alloy substrate was prepared by the sol-gel technique. Scanning electron microscopy and energy-dispersive X-ray analysis results showed the ZnTCP/FHA layer to have a heterogeneous surf...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2007.08.013
更新日期：2008-03-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2007.08.001
更新日期：2008-01-01 00:00:00
abstract：:Atomic force microscopy (AFM) was used to study the morphological changes of two gram-negative pathogens, Pseudomonas aeruginosa and Escherichia coli, after exposure to nitric oxide (NO). The time-dependent effects of NO released from a xerogel coating and the concentration-dependent effects rendered by a small molecu...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.01.025
更新日期：2009-06-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2019.04.046
更新日期：2019-10-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.08.012
更新日期：2014-12-01 00:00:00
abstract：:Human pluripotent stem cells (hPSCs) represent a promising cell source for the development of β-cells for use in therapies for type 1 diabetes. Current culture approaches provide signals to mimic a temporal control of organogenesis to drive the differentiation towards β-cells. However, spatial control may represent an...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.06.032
更新日期：2019-09-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2007.09.011
更新日期：2008-03-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.03.015
更新日期：2012-07-01 00:00:00
abstract：UNLABELLED:Antimicrobial peptides (AMPs) are good candidates to treat burn wounds, a major cause of morbidity, impaired life quality and resources consumption in developed countries. We took advantage of a commercially available hydrogel, Carbopol®, a vehicle for topical administration that maintains a moist environmen...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.07.043
更新日期：2015-10-01 00:00:00
abstract：:Seeking strategies to enhance the overall combinations of mechanical properties is of great significance for engineering materials, but still remains a key challenge because many of these properties are often mutually exclusive. Here we reveal from the perspective of materials science and mechanics that adaptive struc...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.01.010
更新日期：2019-03-01 00:00:00
abstract：:The present work is an amalgamation of computation and experimental approach to gain an insight into composition-structure-bioactivity relationships of alkali-free bioactive glasses in the CaO-MgO-SiO2-P2O5 system. The glasses have been designed in the diopside (CaO·MgO·2SiO2; Di)-tricalcium phosphate (3CaO·P2O5; TCP)...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.01.001
更新日期：2015-03-01 00:00:00
abstract：:Thin film nitinol has unique mechanical properties (e.g., superelasticity), excellent biocompatibility, and ultra-smooth surface, as well as shape memory behavior. All these features along with its low-profile physical dimension (i.e., a few micrometers thick) make this material an ideal candidate in developing low-pr...
journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2015.03.025
更新日期：2015-07-01 00:00:00
abstract：:This study reports the use of variants of coherent anti-Stokes Raman scattering (CARS) microscopy as a novel method for improved physicochemical characterization of drug-loaded silica particles. Ordered mesoporous silica is a biomaterial that can be loaded to carry a number of biochemicals, including poorly water-solu...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.07.021
更新日期：2014-11-01 00:00:00
