Abstract:
BACKGROUND:Porphyromonas gingivalis is a major pathogen and has a high detection rate in periodontal disease. Fimbriae and hemagglutinin are expressed by P. gingivalis, and these play an important role in the adherence of the bacteria to periodontal tissue and biofilm formation. The aim of this study was to investigate the effects of sub-minimal inhibitory concentrations (sub-MICs) of azithromycin on the adherence of P. gingivalis, focusing on the inhibition of fimbriae expression and hemagglutinin activity. METHODS:P. gingivalis ATCC 33277 were incubated anaerobically with sub-MICs of azithromycin at 37°C by gentle shaking for 18 hours. The bacterial cells were harvested, washed twice with phosphate-buffered saline (PBS), and the proteins analyzed by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting. Adherence assay and hemagglutinin activity tests were done with the same culture. RESULTS:The results of SDS-PAGE indicated that the sub-MICs of azithromycin inhibited 41-kDa fimbrial protein expression and hemagglutinin activities. The disappearance of 41-kDa fimbrial protein expression and long fimbriae in 0.4 µg/mL, 0.2 µg/mL, and 0.1 µg/mL of azithromycin was confirmed by western blotting and transmission electron microscopy. The adherence of P. gingivalis to human gingival epithelial cells was reduced by sub-MICs of azithromycin compared with the adherence levels without antibiotic. CONCLUSIONS:These results suggest that sub-MICs of azithromycin may reduce the adherence of P. gingivalis to host cells, by inhibiting production of fimbriae and hemagglutinin activities. Therefore, azithromycin can be used as a biofilm treatment of periodontal disease caused by P. gingivalis.
journal_name
J Periodontoljournal_title
Journal of periodontologyauthors
Kan P,Sasaki H,Inaba K,Watanabe K,Hamada N,Minabe Mdoi
10.1002/JPER.18-0559subject
Has Abstractpub_date
2019-08-01 00:00:00pages
903-910issue
8eissn
0022-3492issn
1943-3670journal_volume
90pub_type
杂志文章abstract:BACKGROUND:Despite previous studies addressing the link between preterm or low birth weight infants and maternal periodontitis, extreme preterm births have received far less attention. This study is designed to address the possible association between maternal periodontal disease and preterm or extreme preterm birth. ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.090527
更新日期:2010-03-01 00:00:00
abstract:SCOPE:These Guidelines apply to products used to promote the regeneration of periodontal tissues lost due to periodontal diseases or other causes. Periodontal regeneration is defined histologically as regeneration of the tooth's supporting tissues, including alveolar bone, periodontal ligament, and cementum over a prev...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1998.69.9.1081
更新日期:1998-09-01 00:00:00
abstract::A previous multi-center study examining patients diagnosed as having at least four periodontally diseased teeth showed that when BANA (N-Benzoyl-DL-Arginine-Naphthylamide) hydrolysis by periodontal pathogens such as Treponema denticola, Porphyromonas gingivalis, and Bacteroides forsythus was evaluated versus clinical ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1993.64.9.848
更新日期:1993-09-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1991.62.8.524
更新日期:1991-08-01 00:00:00
abstract:BACKGROUND:The purpose of this clinical study is to evaluate the radiographic bone remodeling, survival rate, and soft tissue health surrounding a variable-thread tapered implant immediately placed in extraction sites. METHODS:Sixty implants were placed in 55 patients at six centers according to a predetermined protoc...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1902/jop.2013.120638
更新日期:2014-03-01 00:00:00
abstract::The inability to examine initiation and progression of periodontal disease and to assess certain therapies in humans has led to a great interest in the use of animal models in periodontal research. Some of the most prominent animals used are non-human primates. This article reviews the characteristics of non-human pri...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1993.64.6.497
更新日期:1993-06-01 00:00:00
abstract:BACKGROUND:Behçet's disease is a multisystem disorder of unknown etiology, affecting predominantly the oral mucosa, skin, and eyes. Recurrent and painful episodes of oral ulcerations interfere with regular oral hygiene leading to rapid bacterial plaque accumulation. The aims of this study were to evaluate the periodont...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1999.70.12.1449
更新日期:1999-12-01 00:00:00
abstract:BACKGROUND:Chronic periodontitis (CP) exhibits inflammation and alveolar bone loss, and severe forms of periodontitis are suggested to have a genetic basis. Vitamin D receptor (VDR) regulates bone metabolism and inflammation-related genes, and single nucleotide polymorphisms (SNPs) in the VDR gene may affect the functi...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.080465
更新日期:2009-04-01 00:00:00
abstract:BACKGROUND:Regenerative periodontal therapy encompasses use of various bioactive agents that are not only inflammomodulatory but also osteoclast-inhibitory or, rather, osteostimulative. Hypolipidemic statin drugs, particularly rosuvastatin (RSV), are known to be associated with alveolar bone formation and periodontal i...
journal_title:Journal of periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1902/jop.2016.160015
更新日期:2016-12-01 00:00:00
abstract::The American Academy of Periodontology has developed the following parameter on the treatment of chronic periodontitis with slight to moderate loss of periodontal supporting tissues. Patients should be informed of the disease process, therapeutic alternatives, potential complications, expected results, and their respo...
journal_title:Journal of periodontology
pub_type: 指南,杂志文章,实务指引
doi:10.1902/jop.2000.71.5-S.853
更新日期:2000-05-01 00:00:00
abstract:BACKGROUND:Heme oxygenase (HO)-1 is a stress-inducible protein that confers cytoprotection, but its role in gingiva during cyclosporin A (CsA) therapy is unknown. We used in vivo and in vitro models to investigate the expression of mRNA and protein for HO-1 in gingiva upon CsA treatment. METHODS:Twenty-six male Spragu...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2008.080160
更新日期:2008-11-01 00:00:00
abstract:BACKGROUND:The need for keratinized mucosa (KM) or immobile keratinized mucosa (i.e., attached mucosa [AM]) for the maintenance of osseointegrated endosseous dental implants has been controversial. The purpose of this study was to investigate the significance of KM in the maintenance of root-form dental implants with d...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2006.050393
更新日期:2006-08-01 00:00:00
abstract::The dynamics of bone turnover in the furcations of teeth treated with expanded polytetrafluoroethylene (ePTFE) membranes were evaluated using multiple fluorochrome labels in 6 male beagle dogs. Loss of attachment involving the furcation area was induced in the second, third, and fourth premolar teeth using silk ligatu...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1994.65.1.84
更新日期:1994-01-01 00:00:00
abstract::This cross-sectional study examined the gingivitis occurring at puberty in a population of insulin-dependent juvenile diabetics. Seventy-seven children between the ages of 6 and 15 years were examined for gingivitis levels, stages of pubertal maturation and blood levels of glucose and glycosylated hemoglobin. Bacteria...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1983.54.12.714
更新日期:1983-12-01 00:00:00
abstract:BACKGROUND:Platelet-rich plasma (PRP) harbors growth factors identified in bone. It has been suggested that these factors enhance osteogenesis. The objective of this study was to conduct a radiographic evaluation on local bone formation following surgical implantation of a PRP preparation using a critical-size rat calv...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2005.76.8.1287
更新日期:2005-08-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1990.61.5.249
更新日期:1990-05-01 00:00:00
abstract::Unique hydroxyl ion-modulating compounds based on the amino acid glycine have been developed that possess both antimicrobial and pro-healing properties. The purpose of the present study was to determine the effects of one of these compounds, 8.5% (w/v) sodium N, N-bis-2 (hydroxylethyl) aminoacetate (SBHA) with 0.3% (w...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1997.68.5.448
更新日期:1997-05-01 00:00:00
abstract::A small laser doppler probe with an outside diameter comparable to a conventional periodontal probe (0.5 mm) was developed. The objectives of this investigation were to determine if intrasulcular laser doppler readings (LDR): 1) are reproducible; 2) can detect vascular changes associated with trauma from probe placeme...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章
doi:10.1902/jop.1995.66.3.171
更新日期:1995-03-01 00:00:00
abstract::Oral melanin pigmentation is not uncommon in some races and ethnic groups. Repigmentation of depigmented oral mucosa has been described after chemical, thermal, surgical, pharmacologic or idiopathic factors. Information on the repigmentation of oral tissues after surgical procedures is extremely limited. The communica...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1986.57.1.48
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abstract:BACKGROUND:Gingival thickness, keratinized tissue width, and bone morphotype are three important parameters used to categorize periodontal phenotypes. These elements all play an important role in the maintenance of periodontal health. The aim of this review is to explore the importance of converting thin phenotype into...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
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更新日期:2020-03-01 00:00:00
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journal_title:Journal of periodontology
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doi:10.1902/jop.1999.70.8.909
更新日期:1999-08-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章,meta分析
doi:10.1902/jop.2002.73.2.231
更新日期:2002-02-01 00:00:00
abstract::One hundred mandibles of South African Negroes were examined for the presence of intrabony defects, and the distance between the cemento-enamel junction and alveolar bone crest was measured along the buccal and lingual surface of roots associated with interdental craters. Two-hundred thirty-one intrabony defects were ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1984.55.3.149
更新日期:1984-03-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章
doi:10.1902/jop.2004.75.7.1009
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:Different means are available for self-performed oral hygiene. The aim of this study was to evaluate the clinical and microbiologic effects of a preventive homecare program including the combined use of a powered toothbrush and a triclosan/copolymer-containing dentifrice. METHODS:A total of 160 adult subjec...
journal_title:Journal of periodontology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1902/jop.2007.070028
更新日期:2007-09-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1994.65.2.139
更新日期:1994-02-01 00:00:00
abstract::A National Survey of the Oral Health of U.S. children aged 5 to 17 was conducted by the National Institute of Dental Research during the 1986-87 school year. Eleven thousand and seven adolescents aged 14 to 17 years received a periodontal assessment. Their patterns of loss of periodontal attachment as assessed by prob...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1991.62.10.608
更新日期:1991-10-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2013.130389
更新日期:2014-06-01 00:00:00
abstract::In order to increase our understanding of the immunologic aspects of periodontal disease, three groups of extracted human teeth were evaluated for the presence of antibody on the cementum surface. Antibody was demonstrated by direct immunofluorescence using monospecific antibody with human alpha , gamma and upsilon ch...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1982.53.3.168
更新日期:1982-03-01 00:00:00
abstract:BACKGROUND:Prostaglandin E2 (PGE2) plays important roles in the pathogenesis of periodontal disease. Recent studies have revealed the existence of 2 isozymes of cyclooxygenase (COX), called COX-1 and COX-2. The purpose of the present study was to investigate the contribution of COX-1 and COX-2 to PGE2 production by hum...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2000.71.10.1575
更新日期:2000-10-01 00:00:00