Abstract:
:Platelets are crucial for normal hemostasis; however, their hyperactivation also contributes to many potentially lethal pathologies including myocardial infarction, stroke, and cancer. We hypothesized that modified platelets lacking their aggregation and activation capacity could act as reversible inhibitors of platelet activation cascades. Here, we describe the development of detergent-extracted human modified platelets (platelet decoys) that retained platelet binding functions but were incapable of functional activation and aggregation. Platelet decoys inhibited aggregation and adhesion of platelets on thrombogenic surfaces in vitro, which could be immediately reversed by the addition of normal platelets; in vivo in a rabbit model, pretreatment with platelet decoys inhibited arterial injury-induced thromboembolism. Decoys also interfered with platelet-mediated human breast cancer cell aggregation, and their presence decreased cancer cell arrest and extravasation in a microfluidic human microvasculature on a chip. In a mouse model of metastasis, simultaneous injection of the platelet decoys with tumor cells inhibited metastatic tumor growth. Thus, our results suggest that platelet decoys might represent an effective strategy for obtaining antithrombotic and antimetastatic effects.
journal_name
Sci Transl Medjournal_title
Science translational medicineauthors
Papa AL,Jiang A,Korin N,Chen MB,Langan ET,Waterhouse A,Nash E,Caroff J,Graveline A,Vernet A,Mammoto A,Mammoto T,Jain A,Kamm RD,Gounis MJ,Ingber DEdoi
10.1126/scitranslmed.aau5898subject
Has Abstractpub_date
2019-02-13 00:00:00issue
479eissn
1946-6234issn
1946-6242pii
11/479/eaau5898journal_volume
11pub_type
杂志文章abstract::Troublesome involuntary movements in the absence of dopaminergic medication, so-called off-medication dyskinesias, are a serious adverse effect of fetal neural grafts that hinders the development of cell-based therapies for Parkinson's disease. The mechanisms underlying these dyskinesias are not well understood, and i...
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pub_type: 评论,杂志文章
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journal_title:Science translational medicine
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1126/scitranslmed.aaz3426
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pub_type: 杂志文章
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journal_title:Science translational medicine
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pub_type: 临床试验,杂志文章
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doi:10.1126/scitranslmed.aal1321
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pub_type: 杂志文章
doi:10.1126/scitranslmed.3002029
更新日期:2011-03-02 00:00:00
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journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3001249
更新日期:2010-08-25 00:00:00
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doi:10.1126/scitranslmed.aad9735
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journal_title:Science translational medicine
pub_type: 杂志文章
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更新日期:2020-11-18 00:00:00
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pub_type: 评论,杂志文章
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更新日期:2017-07-12 00:00:00
abstract::Elucidating the factors that impinge on the stability of bacterial communities in the vagina may help in predicting the risk of diseases that affect women's health. Here, we describe the temporal dynamics of the composition of vaginal bacterial communities in 32 reproductive-age women over a 16-week period. The analys...
journal_title:Science translational medicine
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