Abstract:
:Adding an electrical pulse to a microfluidic device that squeezes cells through narrow channels efficiently delivers DNA to the nucleus.
journal_name
Sci Transl Medjournal_title
Science translational medicineauthors
King KRdoi
10.1126/scitranslmed.aan0766subject
Has Abstractpub_date
2017-03-22 00:00:00issue
382eissn
1946-6234issn
1946-6242pii
9/382/eaan0766journal_volume
9pub_type
评论,杂志文章abstract::Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections to date has relied heavily on reverse transcription polymerase chain reaction testing. However, limited test availability, high false-negative rates, and the existence of asymptomatic or subclinical infections have resulted in an unde...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.abc1126
更新日期:2020-07-29 00:00:00
abstract::Acute graft-versus-host disease (aGVHD) results from a robust response of donor T cells transferred during hematopoietic stem cell transplantation (HSCT) to allogeneic peptide-major histocompatibility complex antigens. Previous investigations have not identified T cell subsets that selectively mediate either protectiv...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3005452
更新日期:2013-06-05 00:00:00
abstract::The AAA+ adenosine triphosphatase (ATPase) Thorase plays a critical role in controlling synaptic plasticity by regulating the expression of surface α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs). Bidirectional sequencing of exons of ATAD1, the gene encoding Thorase, in a cohort of patients wit...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aah4985
更新日期:2017-12-13 00:00:00
abstract::Cancer-associated thrombocytosis and high concentrations of circulating transforming growth factor-β1 (TGF-β1) are frequently observed in patients with progressive cancers. Using genetic and pharmacological approaches, we show a direct link between thrombin catalytic activity and release of mature TGF-β1 from platelet...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aay4860
更新日期:2020-01-08 00:00:00
abstract::Diseases of failed inflammation resolution are common and largely incurable. Therapeutic induction of inflammation resolution is an attractive strategy to bring about healing without increasing susceptibility to infection. However, therapeutic targeting of inflammation resolution has been hampered by a lack of underst...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3007672
更新日期:2014-02-26 00:00:00
abstract::Obesity promotes breast cancer by enhancing the stiffness of breast adipose tissue through changes in the extracellular matrix (Seo et al., this issue). ...
journal_title:Science translational medicine
pub_type: 评论,杂志文章
doi:10.1126/scitranslmed.aac9446
更新日期:2015-08-19 00:00:00
abstract::Regulatory T cells (T(regs)) manipulated ex vivo have potential as cellular therapeutics in autoimmunity and transplantation. Although it is possible to expand naturally occurring T(regs), an attractive alternative possibility, particularly suited to solid organ and bone marrow transplantation, is the stimulation of t...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3002099
更新日期:2011-05-18 00:00:00
abstract::Elucidating the factors that impinge on the stability of bacterial communities in the vagina may help in predicting the risk of diseases that affect women's health. Here, we describe the temporal dynamics of the composition of vaginal bacterial communities in 32 reproductive-age women over a 16-week period. The analys...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3003605
更新日期:2012-05-02 00:00:00
abstract::Measles virus is a highly infectious morbillivirus responsible for major morbidity and mortality in unvaccinated humans. The related, zoonotic canine distemper virus (CDV) induces morbillivirus disease in ferrets with 100% lethality. We report an orally available, shelf-stable pan-morbillivirus inhibitor that targets ...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3008517
更新日期:2014-04-16 00:00:00
abstract::Whole-genome sequencing of patient DNA can facilitate diagnosis of a disease, but its potential for guiding treatment has been under-realized. We interrogated the complete genome sequences of a 14-year-old fraternal twin pair diagnosed with dopa (3,4-dihydroxyphenylalanine)-responsive dystonia (DRD; Mendelian Inherita...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3002243
更新日期:2011-06-15 00:00:00
abstract::Mycosis fungoides (MF), the most common cutaneous T cell lymphoma (CTCL) is a malignancy of skin-tropic memory T cells. Most MF cases present as early stage (stage I A/B, limited to the skin), and these patients typically have a chronic, indolent clinical course. However, a small subset of early-stage cases develop pr...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aar5894
更新日期:2018-05-09 00:00:00
abstract::Next-generation sequencing technologies have greatly expanded our understanding of cancer genetics. Antisense technology is an attractive platform with the potential to translate these advances into improved cancer therapeutics, because antisense oligonucleotide (ASO) inhibitors can be designed on the basis of gene se...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aac5272
更新日期:2015-11-18 00:00:00
abstract::Long-acting GSK1265744 (GSK744 LA) is a strand transfer inhibitor of the HIV/SIV (simian immunodeficiency virus) integrase and was shown to be an effective preexposure prophylaxis (PrEP) agent in a low-dose intrarectal SHIV (simian-human immunodeficiency virus) rhesus macaque challenge model. We examined the pharmacok...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3010298
更新日期:2015-01-14 00:00:00
abstract::The limited predictability of phase II biomarkers for atherosclerosis outcomes in phase III studies stands in contrast to the number and varied types of biomarkers--soluble, imaging, and functional--that have been used in a diverse array of trials. Although collectively abundant, these biomarker data exist in a fragme...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3002029
更新日期:2011-03-02 00:00:00
abstract::The recent Perspective entitled "Power of rare diseases: Found in translation" undervalues the contributions of academic research in first-in-human studies. ...
journal_title:Science translational medicine
pub_type: 评论,杂志文章
doi:10.1126/scitranslmed.3007911
更新日期:2014-01-15 00:00:00
abstract::Meibomian glands (MGs) are sebaceous glands of the eyelid margin that secrete lipids needed to avert tear evaporation and to help maintain ocular surface homeostasis. Obstruction of MGs or other forms of MG dysfunction can promote chronic diseases of the ocular surface. Although chronic eyelid inflammation, such as al...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aas9164
更新日期:2018-07-25 00:00:00
abstract::Regular physical activity and especially aerobic exercise are associated with reduced risk of disease and enhanced longevity, but the molecular mechanisms of these health benefits remain obscure. A comprehensive metabolomic approach was used to characterize the changes in blood levels of >200 metabolites upon vigorous...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3001188
更新日期:2010-07-21 00:00:00
abstract::Pharmacokinetic and pharmacodynamic models estimate the potency of antiviral agents but do not capture viral and immunologic factors that drive the natural dynamics of infection. We designed a mathematical model that synthesizes pharmacokinetics, pharmacodynamics, and viral pathogenesis concepts to simulate the activi...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aad6654
更新日期:2016-02-03 00:00:00
abstract::The U.S.-Latin America Cancer Research Network strives to expand translational research capabilities and forge new collaborations. ...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aad5859
更新日期:2015-12-23 00:00:00
abstract::High doses of oral vitamin D3 attenuate skin inflammation following experimentally induced sun damage. ...
journal_title:Science translational medicine
pub_type: 评论,杂志文章
doi:10.1126/scitranslmed.aan5233
更新日期:2017-06-14 00:00:00
abstract::Patient-derived xenografts (PDXs) and PDX-derived cells (PDCs) are useful in preclinical research. We performed a drug screening assay using PDCs and identified proteasome inhibitors as promising drugs for cholangiocarcinoma (CCA) treatment. Furthermore, we determined that phosphate and tensin homology deleted on chro...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aay0152
更新日期:2020-09-23 00:00:00
abstract::Arrhythmogenic cardiomyopathy (ACM) is characterized by frequent cardiac arrhythmias. To elucidate the underlying mechanisms and discover potential chemical modifiers, we created a zebrafish model of ACM with cardiac myocyte-specific expression of the human 2057del2 mutation in the gene encoding plakoglobin. A high-th...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3008008
更新日期:2014-06-11 00:00:00
abstract::Vaccines directed against the blood stages of Plasmodium falciparum malaria are intended to prevent the parasite from invading and replicating within host cells. No blood-stage malaria vaccine has shown clinical efficacy in humans. Most malaria vaccine antigens are parasite surface proteins that have evolved extensive...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3000257
更新日期:2009-10-14 00:00:00
abstract::Graft-versus-host disease (GVHD) is the most common complication of hematopoietic stem cell transplant (HCT). However, our understanding of the molecular pathways that cause this disease remains incomplete, leading to inadequate treatment strategies. To address this, we measured the gene expression profile of nonhuman...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aad3231
更新日期:2015-11-25 00:00:00
abstract::Airway mucus in cystic fibrosis (CF) is highly elastic, but the mechanism behind this pathology is unclear. We hypothesized that the biophysical properties of CF mucus are altered because of neutrophilic oxidative stress. Using confocal imaging, rheology, and biochemical measures of inflammation and oxidation, we foun...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3010525
更新日期:2015-02-25 00:00:00
abstract::Translation in an academic environment requires a support system--people, goals, models, partnerships, and infrastructures--that will push promising basic science and technology projects forward into the clinic. ...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aaa2049
更新日期:2015-04-08 00:00:00
abstract::Platelets are crucial for normal hemostasis; however, their hyperactivation also contributes to many potentially lethal pathologies including myocardial infarction, stroke, and cancer. We hypothesized that modified platelets lacking their aggregation and activation capacity could act as reversible inhibitors of platel...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aau5898
更新日期:2019-02-13 00:00:00
abstract::Disease-free infection in HIV-infected adults is associated with human leukocyte antigen-mediated suppression of viremia, whereas in the sooty mangabey and other healthy natural hosts of simian immunodeficiency virus (SIV), viral replication continues unabated. To better understand factors preventing HIV disease, we i...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.aag1048
更新日期:2016-09-28 00:00:00
abstract::Alzheimer's disease (AD) is hypothesized to be caused by an overproduction or reduced clearance of amyloid-β (Aβ) peptide. Autosomal dominant AD (ADAD) caused by mutations in the presenilin (PSEN) gene have been postulated to result from increased production of Aβ42 compared to Aβ40 in the central nervous system (CNS)...
journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3005615
更新日期:2013-06-12 00:00:00
abstract::Pathogenic immunoglobulin G (IgG) autoantibodies characterize some human autoimmune diseases; their high concentration and long half-life are dependent on recycling by the neonatal Fc receptor (FcRn). Inhibition of FcRn is an attractive new treatment concept for IgG-mediated autoimmune diseases. Rozanolixizumab (UCB76...
journal_title:Science translational medicine
pub_type: 杂志文章,随机对照试验
doi:10.1126/scitranslmed.aan1208
更新日期:2017-11-01 00:00:00