Abstract:
:Motor neuron-specific microRNA-218 (miR-218) has recently received attention because of its roles in mouse development. However, miR-218 relevance to human motor neuron disease was not yet explored. Here, we demonstrate by neuropathology that miR-218 is abundant in healthy human motor neurons. However, in amyotrophic lateral sclerosis (ALS) motor neurons, miR-218 is down-regulated and its mRNA targets are reciprocally up-regulated (derepressed). We further identify the potassium channel Kv10.1 as a new miR-218 direct target that controls neuronal activity. In addition, we screened thousands of ALS genomes and identified six rare variants in the human miR-218-2 sequence. miR-218 gene variants fail to regulate neuron activity, suggesting the importance of this small endogenous RNA for neuronal robustness. The underlying mechanisms involve inhibition of miR-218 biogenesis and reduced processing by DICER. Therefore, miR-218 activity in motor neurons may be susceptible to failure in human ALS, suggesting that miR-218 may be a potential therapeutic target in motor neuron disease.
journal_name
Sci Transl Medjournal_title
Science translational medicineauthors
Reichenstein I,Eitan C,Diaz-Garcia S,Haim G,Magen I,Siany A,Hoye ML,Rivkin N,Olender T,Toth B,Ravid R,Mandelbaum AD,Yanowski E,Liang J,Rymer JK,Levy R,Beck G,Ainbinder E,Farhan SMK,Lennox KA,Bode NM,Behlke MA,doi
10.1126/scitranslmed.aav5264subject
Has Abstractpub_date
2019-12-18 00:00:00issue
523eissn
1946-6234issn
1946-6242pii
11/523/eaav5264journal_volume
11pub_type
杂志文章abstract::Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder. We screened 751 familial ALS patient whole-exome sequences and identified six mutations including p.D40G in the ANXA11 gene in 13 individuals. The p.D40G mutation was absent from 70,000 control whole-exome sequences. This mutation segregated wi...
journal_title:Science translational medicine
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journal_title:Science translational medicine
pub_type: 杂志文章,评审
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journal_title:Science translational medicine
pub_type: 杂志文章
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更新日期:2016-01-20 00:00:00
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journal_title:Science translational medicine
pub_type: 评论,杂志文章
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更新日期:2017-01-11 00:00:00
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journal_title:Science translational medicine
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doi:10.1126/scitranslmed.aax1744
更新日期:2020-03-18 00:00:00
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journal_title:Science translational medicine
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更新日期:2018-08-29 00:00:00
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journal_title:Science translational medicine
pub_type: 杂志文章
doi:10.1126/scitranslmed.3002099
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journal_title:Science translational medicine
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journal_title:Science translational medicine
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abstract::Depending on age of acquisition, hepatitis B virus (HBV) can induce a cell-mediated immune response that results in either cure or progressive liver injury. In adult-acquired infection, HBV antigens are usually cleared, whereas in infancy-acquired infection, they persist. Individuals infected during infancy therefore ...
journal_title:Science translational medicine
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journal_title:Science translational medicine
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journal_title:Science translational medicine
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