Abstract：
:Non-small cell lung cancer (NSCLC) is still an unresolved source of tumor-related death internationally. Current studies have discovered that microRNAs (miRNAs) are associated with diverse cancers development, including NSCLC. Our paper focused on the functional character of miR-4286 in NSCLC. miR-4286 level in 68 cases of NSCLC tissues, matched neighboring nontumor tissues and different cancer cell lines were inspected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The connection concerning miR-4286 expression and clinicopathological features of patients with NSCLC were further determined. After knockdown or overexpression of miR-4286, cell viability, cell cycle, and/or apoptotic cells were examined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assay, respectively. Moreover, the cell cycle- and apoptosis-related proteins were estimated by qRT-PCR and Western blot. In comparison with the matched nontumor tissues, miR-4286 was significantly enhanced in lung malignancy tissues and different cell lines. miR-4286 expression was related with the tumor-node-metastasis stage, lymphatic metastasis, and distant metastasis. Cell viability was ominously weakened by suppression of miR-4286 in A549 cells, whereas was statistically upregulated by overexpression of miR-4286 in NCI-H1299 cells. Additionally, we detected that suppression of miR-4286 tempted cell cycle arrest in G1 stage and fortified apoptosis in A549 cells. Runx3 was recognized as one target gene of miR-4286, and the impacts of suppression of miR-4286 on cell viability and apoptosis were through regulation of Runt-related transcription factor 3. Our study suggests that miR-4286 overexpression represents a tumor promoter role in NSCLC cells.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
An X,Ge J,Guo H,Mi H,Zhou J,Liu Y,Weiyue,Wu Zdoi
10.1002/jcb.29024subject
Has Abstractpub_date
2019-10-01 00:00:00pages
17573-17583issue
10eissn
0730-2312issn
1097-4644journal_volume
120pub_type
杂志文章abstract：:Breast cancer remains the major cause of cancer-related deaths in women world-wide. The heterogeneity of breast cancer has further complicated the progress of target-based therapies. Triple negative breast cancers, lacking estrogen receptor, progesterone receptor and the Her-2/neu (ErbB2), represent a highly aggressiv...
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更新日期：2005-12-15 00:00:00
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更新日期：2010-08-01 00:00:00
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更新日期：1985-01-01 00:00:00
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更新日期：2004-01-01 00:00:00
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更新日期：1999-12-01 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
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更新日期：2005-04-01 00:00:00
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更新日期：2018-09-23 00:00:00
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journal_title：Journal of cellular biochemistry
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更新日期：2015-12-01 00:00:00
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更新日期：2009-09-01 00:00:00
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更新日期：2015-08-01 00:00:00
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更新日期：2017-12-01 00:00:00
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