Abstract：
:Cell-free circulating DNA (cf-DNA) can be detected by various of laboratory techniques. We described a branched DNA-based Alu assay for measuring cf-DNA in septic patients. Compared to healthy controls and systemic inflammatory response syndrome (SIRS) patients, serum cf-DNA levels were significantly higher in septic patients (1426.54 ± 863.79 vs 692.02 ± 703.06 and 69.66 ± 24.66 ng/mL). The areas under the receiver operating characteristic curve of cf-DNA for normal vs sepsis and SIRS vs sepsis were 0.955 (0.884-1.025), and 0.856 (0.749-0.929), respectively. There was a positive correlation between cf-DNA and interleukin 6 or procalcitonin or Acute Physiology and Chronic Health Evaluation II. The cf-DNA concentration was higher in intensive care unit nonsurviving patients compared to surviving patients (2183.33 ± 615.26 vs 972.46 ± 648.36 ng/mL; P < .05). Branched DNA-based Alu assays are feasible and useful to quantify serum cf-DNA levels. Increased cf-DNA levels in septic patients might complement C-reactive protein and procalcitonin in a multiple marker format. Cell-free circulating DNA might be a new marker in discrimination of sepsis and SIRS.
journal_name
J Crit Carejournal_title
Journal of critical careauthors
Hou YQ,Liang DY,Lou XL,Zhang M,Zhang ZH,Zhang LRdoi
10.1016/j.jcrc.2015.10.013subject
Has Abstractpub_date
2016-02-01 00:00:00pages
90-5issue
1eissn
0883-9441issn
1557-8615pii
S0883-9441(15)00542-0journal_volume
31pub_type
杂志文章abstract：PURPOSE:Patients with severe penetrating head injury often have a coagulopathy that is difficult to correct. In this report, we describe 3 such patients who were treated with activated factor VII (FVIIa) to stop ongoing hemorrhage that was refractory to conventional treatment. SUBJECTS AND METHODS:We treated 3 patient...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2005.02.004
更新日期：2005-06-01 00:00:00
abstract：PURPOSE:Frailty in critical illness is common and associated with poor outcomes, however little is known about contributing factors. We compared the Clinical Frailty Scale (CFS) with a multi-dimensional validated tool, the Edmonton Frail Scale (EFS), and investigated which health domains are affected by frailty in ICU....
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2019.04.032
更新日期：2019-08-01 00:00:00
abstract：PURPOSE:To compare the quality of data recorded by a commercially available clinical information system (CIS) to other commonly used methods for obtaining large amounts of patient data. MATERIALS AND METHODS:Five sets of clinical patient data were chosen as a cross-section of all the data collected by a CIS in our int...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2004.02.003
更新日期：2004-03-01 00:00:00
abstract：PURPOSE:Urinary excretion of chloride corrects metabolic acidosis, but this may be hampered in patients with impaired renal function. We explored the effects of renal function on acid-base characteristics and urinary strong ion excretion using the Stewart approach in critically ill patients with metabolic acidosis. MA...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2011.05.028
更新日期：2012-06-01 00:00:00
abstract：BACKGROUND:Non-invasive mechanical ventilation (NIV) is a standard respiratory support technique used in intensive care units. High-Flow Nasal Cannula (HFNC) has emerged as an alternative, but further evidence is needed. The lung aeration and diaphragm changes achieved with these two strategies in healthy subjects have...
journal_title：Journal of critical care
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jcrc.2019.06.009
更新日期：2019-10-01 00:00:00
abstract：BACKGROUND:The optimal mode of ventilation in acute respiratory distress syndrome (ARDS) remains uncertain. Airway pressure release ventilation (APRV) is a recognized treatment for mechanically-ventilated patients with severe hypoxaemia. However, contemporary data on its role as a rescue modality in ARDS is lacking. Th...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2016.03.002
更新日期：2016-08-01 00:00:00
abstract：:The aim was to explore how relatives and physicians understood cases where organ donation had been requested and what factors were salient for the decision on donation. Physicians of 25 deceased patients and 20 relatives were interviewed. The material was analyzed using qualitative methods. Eleven patients had declare...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2007.03.002
更新日期：2007-12-01 00:00:00
abstract：PURPOSE:We determined the prevalence of risk factors for the development of acute respiratory distress syndrome (ARDS), outcomes of critical illness, and the impact of highly active antiretroviral therapy in HIV-1-infected patients. We hypothesized that in an urban county hospital, HIV-1-infected patients with ARDS wou...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2014.10.020
更新日期：2015-02-01 00:00:00
abstract：:Sixty-nine patients undergoing liver transplantation were evaluated to elucidate the relationship between hypotension and physiological changes seen on reperfusion of the grafted liver. Measured variables included hemodynamic profiles, core temperature, serum potassium, ionized calcium levels, arterial blood-gas tensi...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/0883-9441(93)90021-c
更新日期：1993-09-01 00:00:00
abstract：PURPOSE:Echocardiography is increasingly being taught to intensive care unit residents. Current training methods require teachers to closely supervise trainees individually, and are time-intensive. To reduce the time burden, dyad training (training in pairs) under simulation conditions has been shown to be non-inferior...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2018.10.009
更新日期：2019-02-01 00:00:00
abstract：INTRODUCTION:The aim of our study was to compare the reliability and safety of the classical I-AT with the continuous positive airway pressure apnea test (CPAP-AT). MATERIAL AND METHODS:In the group of 48 patients (group O), an I-AT was performed at the end of BD diagnostic procedures, and approximately 1-1.5h later C...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2017.10.038
更新日期：2018-04-01 00:00:00
abstract：PURPOSE:To evaluate the impact of slow-tempo music listening periods in mechanically ventilated intensive care unit patients. METHODS:A randomized crossover study was performed in a 16-bed, adult critical care unit at a tertiary care hospital. Still-sedated patients, mandating at least 3 more days of mechanical ventil...
journal_title：Journal of critical care
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jcrc.2013.01.007
更新日期：2013-08-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to refine and validate an automatic risk of unplanned readmission (Stability and Workload Index for Transfer, or SWIFT) calculator in a prospective cohort of consecutive medical intensive care unit (ICU) patients in a teaching hospital with comprehensive electronic medical records (E...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2011.05.003
更新日期：2011-12-01 00:00:00
abstract：PURPOSE:Sepsis is a lethal outcome of the inflammation and coagulation process. Human interleukin (IL)-6 and tumor necrosis factor (TNF) α are well-known inflammation factors closely associated with sepsis. In the present study, we aim to investigate the association of promoter-region polymorphisms IL-6 (-174G/C) rs180...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2015.04.123
更新日期：2015-10-01 00:00:00
abstract：PURPOSE:The goal of this study was to determine the impact of surgical rib fixation (SRF) in a treatment protocol for severe blunt chest trauma. MATERIALS AND METHODS:Patients with flail chest admitted between September 2009 and June 2010 to our level I trauma center who failed traditional management and underwent SRF...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2013.08.003
更新日期：2014-02-01 00:00:00
abstract：PURPOSE:The purpose was to determine prognosis of patients presenting extreme acidosis (pH <7) on admission to the intensive care unit (ICU) and to identify mortality risk factors. MATERIALS AND METHODS:We retrospectively analyzed all patients who presented with extreme acidosis within 24 hours of admission to a polyv...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2015.09.025
更新日期：2016-02-01 00:00:00
abstract：PURPOSE:Few guidelines exist regarding the selection of a particular type or size of tracheostomy tube. Although nonstandard tubes can be placed over the percutaneous kit dilator, clinicians often place standard tracheostomy tubes and change to nonstandard tubes only after problems arise. This practice risks early trac...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2016.05.025
更新日期：2017-02-01 00:00:00
abstract：PURPOSE:The purpose of this study is to examine the relationship between the occurrence of a difficult intubation and (1) the use of neuromuscular blocking agents (NMB) and (2) the presence of airway injuries. It is a retrospective analysis of data from a trauma registry. MATERIALS AND METHODS:Registry records of pati...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/s0883-9441(98)90022-3
更新日期：1998-03-01 00:00:00
abstract：PURPOSE:Automated weaning modes are available in some mechanical ventilators, but no studies compared them hitherto. We compared the performance of 3 automated modes under standard and challenging situations. METHODS:We used a lung simulator to compare 3 automated modes, adaptive support ventilation (ASV), mandatory r...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2011.12.021
更新日期：2012-12-01 00:00:00
abstract：PURPOSE:Little is known about the relationship between anxiety and pain in intensive care unit (ICU) patients despite its importance. The aims of the present study are to examine the correlation between pain and anxiety during ICU care and to investigate its effects on the dose of opioids and anxiolytics administered. ...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2015.05.025
更新日期：2015-10-01 00:00:00
abstract：PURPOSE:Evaluation of the effects of nimodipine administration during and after cardiopulmonary resuscitation (CPR) on oxygen delivery and consumption was the aim of this study. METHODS:A randomized double-blind study in 32 anesthetized domestic pigs was performed. After 5 minutes of ventricular fibrillation (VF) and ...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/0883-9441(94)90029-9
更新日期：1994-03-01 00:00:00
abstract：HYPOTHESIS:As vasopressin is a small peptide, its sieving coefficient (SC) and clearance (CL) during continuous renal replacement therapy may be intermediate to those for urea and β2 microglobulin (commonly used markers for small- and middle-molecular weight solutes, respectively). METHODS:A prospective, minimal-risk ...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2010.03.005
更新日期：2010-12-01 00:00:00
abstract：PURPOSE:Developing countries carry the greatest burden of sepsis, yet few descriptive data exist from the Western Hemisphere. We conducted a retrospective cohort study to elucidate the presentation, treatment, and outcomes of sepsis at an urban referral hospital in Port-au-Prince, Haiti. MATERIALS AND METHODS:We studi...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2016.09.031
更新日期：2017-04-01 00:00:00
abstract：PURPOSE:Newly diagnosed swallowing dysfunction is rare, with an incidence <1% in hospitalized patients. The purpose of this study was to evaluate the incidence and clinical characteristics of dysphagia in elderly trauma patients specifically. METHODS:Patients ≥75years who had newly diagnosed swallowing dysfunction wer...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2017.07.027
更新日期：2017-12-01 00:00:00
abstract：INTRODUCTION:The pathophysiology of sepsis-associated encephalopathy (SAE) is not entirely clear, but one of the possible underlying mechanisms is the alteration of the cerebral microvascular function. The aim of the present work was to test whether cerebral vasomotor reactivity is impaired in patients with severe seps...
journal_title：Journal of critical care
pub_type： 临床试验,杂志文章
doi：10.1016/j.jcrc.2011.11.002
更新日期：2012-08-01 00:00:00
abstract：PURPOSE:To describe the prevalence of hypocapnia and hypercapnia during the earliest period of mechanical ventilation, and determine the association between PaCO2 and mortality. MATERIALS AND METHODS:A cohort study using an emergency department registry of mechanically ventilated patients. PaCO2 was categorized: hypoc...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2017.04.033
更新日期：2017-10-01 00:00:00
abstract：INTRODUCTION:Heart rate complexity, commonly described as a "new vital sign," has shown promise in predicting injury severity, but its use in clinical practice is not yet widely adopted. We previously demonstrated the ability of this noninvasive technology to predict lifesaving interventions (LSIs) in trauma patients. ...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2015.03.018
更新日期：2015-08-01 00:00:00
abstract：PURPOSE:Nebulized heparin may reduce fibrin cast formation and reduce the degree of airway obstruction in burn inhalation injury. METHODS:Twenty-nine patients admitted to burn intensive care unit (ICU) within 24 hours of burn inhalation injury were included in this prospective double-blinded randomized study. Group H5...
journal_title：Journal of critical care
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jcrc.2013.06.017
更新日期：2014-02-01 00:00:00
abstract：PURPOSE:Alcohol-use disorders (AUDs) have been associated with increased sepsis-related mortality. As patients with AUDs are often thiamine deficient, we investigated practice patterns relating to thiamine administration in patients with AUDs presenting with septic shock and explored the association between receipt of ...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2017.08.022
更新日期：2018-02-01 00:00:00
abstract：:Critical care physicians may hear a surrogate decision-maker ask, "What would you do if she was your mother?" or "What if your father was this sick?" These kinds of questions ask more of the critical care physician than the surrogate might realize. There are deep-seated ethical, professional, and personal complexities...
journal_title：Journal of critical care
pub_type： 杂志文章
doi：10.1016/j.jcrc.2018.10.031
更新日期：2019-02-01 00:00:00
