Abstract：
OBJECTIVE:Pulmonary surfactant-associated protein B (SP-B), which is synthesized and secreted by alveolar epithelial type II cells, is crucial for normal functioning of pulmonary surfactant. Degeneration of pulmonary surfactant is the essential cause of acute lung injury (ALI). ALI is often studied in animal models using oleic acid, and the effects of oleic acid on pulmonary surfactant and SP-B are not clear. In this study, we examined the effects of oleic acid on the A549 cell line which resembles the alveolar epithelial type II cells. MATERIALS AND METHODS:A549 cells were exposed for 24 hours to 300, 400, 500 or 600 µM of oleic acid. Cell morphological changes were observed using an inverted microscope, and cell proliferation was quantified with the Cell Counting Kit-8. Extracellular SP-B levels were assessed by ELISA, whereas intracellular SP-B expression by Western blot. RESULTS:Oleic acid caused dose-dependent changes in cell morphology of A549 cells and decreased their proliferation. This was accompanied by release of SP-B into extracellular supernatants and corresponding decrease of intracellular levels of this protein. CONCLUSIONS:Oleic acid causes a dose-dependent injury to A549 cells, release of SP-B into extracellular compartment, and decrease of intracellular SP-B expression. Our findings provide mechanistic insights into animal modeling of ALI with oleic acid.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Zhou DJ,Chen Y,Zhang XJ,Ma C,Qiu J,Zhou JHsubject
Has Abstractpub_date
2015-09-01 00:00:00pages
3438-43issue
18eissn
1128-3602issn
2284-0729journal_volume
19pub_type
杂志文章abstract：OBJECTIVE:Accumulating studies have reported that circular RNAs (circRNAs) can act as novel prognostic biomarkers in multiple malignant tumors. Here, we conducted a study to investigate the potential function and molecular mechanism of action of hsa_circ_0010882 in gastric cancer (GC). PATIENTS AND METHODS:The express...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202002_20165
更新日期：2020-02-01 00:00:00
abstract：BACKGROUND:Circadian rhythms tend to change as animals age; however, the molecular mechanisms underlying these are not yet fully understood. OBJECTIVE:To investigate whether the DNA methylation of clock genes changes with age and contributes to circadian dysfunction in aged animals. METHODS:We examined the methylatio...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
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更新日期：2013-07-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect and mechanism of bone marrow stem cells (BMSCs) modified with CXCR-4 gene combined with stromal derived factor-1α (SDF-1α) in the treatment of acute spinal cord injury (SCI) in rats. MATERIALS AND METHODS:CXCR-4 gene was transfected by a virus. Spinal cord injury rats were randomly div...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
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更新日期：2017-01-01 00:00:00
abstract：OBJECTIVE:Perlecan, which is also called heparan sulfate proteoglycan 2 (HSPG2), is a protein encoded by the HSPG2 gene that maps to 1p36.12 in the human genome. In this study, we assessed the independent prognostic value of HSPG2 in terms of overall survival (OS) and recurrence-free survival (RFS) in patients with LGG...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201810_16154
更新日期：2018-10-01 00:00:00
abstract：OBJECTIVE:ACE2 long served as the human gateway for multiple coronaviruses, including the currently pandemic SARS-CoV-2. This mini-review explores the potential of targeting ACE2 in blocking viral penetrance. MATERIALS AND METHODS:PubMed search was conducted using the terms: "coronaviridae", "peptidyl-dipeptidase A", ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：10.26355/eurrev_202009_23067
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVE:It has been confirmed that the dysregulation of long noncoding RNAs (lncRNAs) is associated with various diseases, especially cancer. LncRNA MAGI2 antisense RNA 3 (MAGI2-AS3) has been reported to be involved in the progression of bladder cancer and breast cancer. In this study, we aimed to explore its express...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201904_17710
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202002_20379
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：10.26355/eurrev_202004_21046
更新日期：2020-04-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
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更新日期：2010-08-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201903_17391
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doi：10.26355/eurrev_202007_22226
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-11-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202011_23610
更新日期：2020-11-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2010-05-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
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doi：10.26355/eurrev_202003_20700
更新日期：2020-03-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2006-01-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2012-11-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
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journal_title：European review for medical and pharmacological sciences
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更新日期：2012-10-01 00:00:00
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pub_type： 杂志文章,随机对照试验
doi：
更新日期：2016-03-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-06-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2009-11-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201912_19761
更新日期：2019-12-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
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更新日期：2017-06-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
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doi：
更新日期：2017-04-01 00:00:00
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journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
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