当前位置： SCI文献检索 > JOURNAL OF INORGANIC BIOCHEMISTRY期刊下所有文献 > Molar absorption coefficients and stability constants of metal complexes of 4-(2-pyridylazo)resorcinol (PAR): Revisiting common chelating probe for the study of metalloproteins.

Molar absorption coefficients and stability constants of metal complexes of 4-(2-pyridylazo)resorcinol (PAR): Revisiting common chelating probe for the study of metalloproteins.

Abstract：

:4-(2-Pyridylazo)resorcinol (PAR) is one of the most popular chromogenic chelator used in the determination of the concentrations of various metal ions from the d, p and f blocks and their affinities for metal ion-binding biomolecules. The most important characteristics of such a sensor are the molar absorption coefficient and the metal-ligand complex dissociation constant. However, it must be remembered that these values are dependent on the specific experimental conditions (e.g. pH, solvent components, and reactant ratios). If one uses these values to process data obtained in different conditions, the final result can be under- or overestimated. We aimed to establish the spectral properties and the stability of PAR and its complexes accurately with Zn(2+), Cd(2+), Hg(2+), Co(2+), Ni(2+), Cu(2+), Mn(2+) and Pb(2+) at a multiple pH values. The obtained results account for the presence of different species of metal-PAR complexes in the physiological pH range of 5 to 8 and have been frequently neglected in previous studies. The effective molar absorption coefficient at 492 nm for the ZnHx(PAR)2 complex at pH7.4 in buffered water solution is 71,500 M(-1) cm(-1), and the dissociation constant of the complex in these conditions is 7.08×10(-13) M(2). To confirm these values and estimate the range of the dissociation constants of zinc-binding biomolecules that can be measured using PAR, we performed several titrations of zinc finger peptides and zinc chelators. Taken together, our results provide the updated parameters that are applicable to any experiment conducted using inexpensive and commercially available PAR.

journal_name

J Inorg Biochem

journal_title

Journal of inorganic biochemistry

authors

Kocyła A,Pomorski A,Krężel A

doi

10.1016/j.jinorgbio.2015.08.024

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

82-92

eissn

0162-0134

issn

1873-3344

pii

S0162-0134(15)30069-6

journal_volume

152

pub_type

杂志文章

相关文献

JOURNAL OF INORGANIC BIOCHEMISTRY文献大全
  • Bis(L-cysteinato)gold(I): chemical characterization and identification in renal cortical cytoplasm.

    abstract：:L-Cysteinatogold(I) was prepared by the reaction of L-cysteine with KAuBr4 in acidic media and its solubility determined from pH 4 to 10. The solubility at pH 7.4 and 37 degrees C is 1 microM. In the presence of excess cysteine, the solubility increases because of formation of bis(L-cysteinato)gold(I). The equilibrium...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0162-0134(00)80198-1

    authors： Shaw CF 3rd,Schmitz G,Thompson HO,Witkiewicz P

    更新日期：1979-07-01 00:00:00

  • A singular value decomposition approach for kinetic analysis of reactions of HNO with myoglobin.

    abstract：:The reactions of several horse heart myoglobin species with nitrosyl hydride, HNO, derived from Angeli's salt (AS) and Piloty's acid (PA) have been followed by UV-visible, (1)H NMR and EPR spectroscopies. Spectral analysis of myoglobin-derived speciation during the reactions was obtained by using singular value decomp...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2012.10.005

    authors： Zapata AL,Kumar MR,Pervitsky D,Farmer PJ

    更新日期：2013-01-01 00:00:00

  • QM/MM investigation of structure and spectroscopic properties of a vanadium-containing peroxidase.

    abstract：:We present a comprehensive analysis of the most likely ground state configuration of the resting state of vanadium dependent chloroperoxidase (VCPO) based on quantum mechanics/molecular mechanics (QM/MM) evaluations of ground state properties, UV-vis spectra and NMR chemical shifts. Within the QM/MM framework, density...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2008.04.006

    authors： Zhang Y,Gascón JA

    更新日期：2008-08-01 00:00:00

  • Exploring anticancer efficiency of mitochondria-targeted cyclometalated iridium(III) complexes.

    abstract：:We prepared and characterized new iridium(III) complexes: [Ir(NC)2(MPPIP)](PF6) (N-C = 2-phenylpyridine 1; benzo[h]quinolone 2; 1-phenylisoquinolone, 3, MPPIP = 2-(4-(4'-methylpiperazin-yl)phenyl)-1H-imidazo[4,5-f][1,10]phenanthroline). MTT (MTT = 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide) method ...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2020.111215

    authors： Gu Y,Wen H,Bai L,Zhou Y,Zhang H,Tian L,Zhang Y,Hao J,Liu Y

    更新日期：2020-11-01 00:00:00

  • Effect of polyanions on the spectroscopic properties of the nitric oxide derivative of ferrous dromedary (Camelus dromedarius) hemoglobin.

    abstract：:The effect of inositol hexakisphosphate, 2,3-diphosphoglycerate, dextran sulphate, and heparin on the spectroscopic (absorbance, circular dichroism, EPR) properties of the nitric oxide derivative of ferrous dromedary (Camelus dromedarius) hemoglobin was investigated. The results obtained show that: (i) all polyanions ...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/0162-0134(88)85001-3

    authors： Ascenzi P,Santucci R,Desideri A,Amiconi G

    更新日期：1988-04-01 00:00:00

  • Nanomolar aluminum induces pro-inflammatory and pro-apoptotic gene expression in human brain cells in primary culture.

    abstract：:Aluminum, the most abundant neurotoxic metal in our biosphere, has been implicated in the etiology of several neurodegenerative disorders including Alzheimer's disease (AD). To further understand aluminum's influence on gene expression, we examined total messenger RNA levels in untransformed human neural cells exposed...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2005.04.021

    authors： Lukiw WJ,Percy ME,Kruck TP

    更新日期：2005-09-01 00:00:00

  • A role for cyclic hydroxamates in aluminium resistance in maize?

    abstract：:Hydroxamate siderophores have been found to alleviate Al toxicity in bacteria. In Poaceae plants cyclic hydroxamates, like DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one) and its derivatives have mostly been studied in relation to either defence against insects or allelopathy. In this study the influence of Al o...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2005.05.017

    authors： Poschenrieder C,Tolrà RP,Barceló J

    更新日期：2005-09-01 00:00:00

  • New photosensitive nanometric graphite oxide composites as antimicrobial material with prolonged action.

    abstract：:A new conjugate material based on partially reduced graphite oxide (rGO), silver nanoparticles (Ag), and bis(lysinato)zirconium(IV) phthalocyanine complex (ZrPc) was obtained. Its optical properties (absorption and photoluminescence) after dispersion in solvents were examined. The antimicrobial properties were tested ...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2016.02.019

    authors： Gerasymchuk Y,Lukowiak A,Wedzynska A,Kedziora A,Bugla-Ploskonska G,Piatek D,Bachanek T,Chernii V,Tomachynski L,Strek W

    更新日期：2016-06-01 00:00:00

  • Future perception in P450 research.

    abstract：:Cytochrome P450 (P450) of eukaryotes and prokaryotes diversified remarkably during the long course of their evolution. Since the diversification of P450 was the consequence of the adaptation of various living organisms to diverse environmental changes, the catalytic activities and physiological functions of the P450s ...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2018.06.002

    authors： Omura T

    更新日期：2018-09-01 00:00:00

  • Subcellular distribution of copper in the kidneys of normal, copper-poisoned, and thiomolybdate-treated sheep.

    abstract：:Twenty-seven sheep given either copper (Cu) and/or tetrathiomolybdate (TM) were used to study the subcellular distribution of Cu within the kidney and to monitor the location of lysosomes within the subcellular fractions using acid phosphatase (AP) as a marker enzyme. Copper dosing alone increased the Cu content in th...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/0162-0134(89)84003-6

    authors： Gooneratne SR,Howell JM,Gawthorne JM,Kumaratilake JS

    更新日期：1989-01-01 00:00:00

  • Structures of asymmetric complexes of human neuron specific enolase with resolved substrate and product and an analogous complex with two inhibitors indicate subunit interaction and inhibitor cooperativity.

    abstract：:In the presence of magnesium, enolase catalyzes the dehydration of 2-phospho-d-glycerate (PGA) to phosphoenolpyruvate (PEP) in glycolysis and the reverse reaction in gluconeogensis at comparable rates. The structure of human neuron specific enolase (hNSE) crystals soaked in PGA showed that the enzyme is active in the ...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2012.02.011

    authors： Qin J,Chai G,Brewer JM,Lovelace LL,Lebioda L

    更新日期：2012-06-01 00:00:00

  • Improved syntheses of β-octabromo-meso-triarylcorrole derivatives.

    abstract：:In spite of significant applications as starting materials for a variety of metallocorrole derivatives, free-base β-octabromo-meso-triarylcorroles continue to be viewed as inaccessible. The reasons range from the need for tedious column-chromatographic purification to limitations of the reductive demetallation protoco...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2015.07.017

    authors： Capar J,Berg S,Thomas KE,Beavers CM,Gagnon KJ,Ghosh A

    更新日期：2015-12-01 00:00:00

  • In vitro and in vivo antitumour activity and cellular pharmacological properties of new platinum-iminoether complexes with different configuration at the iminoether ligands.

    abstract：:In order to widen our knowledge on antitumour trans-[PtCl2(iminoether)2] complexes, we have synthesised two new derivatives, trans-[PtCl2¿E-HN = C(OEt)Me¿2] (1) and trans-[PtCl2¿Z-HN = C(OEt)Me¿2] (2), which differ in the configuration of the iminoether ligands. Isomer 1 showed an in vitro cytotoxicity similar to that...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0162-0134(99)00139-7

    authors： Coluccia M,Nassi A,Boccarelli A,Giordano D,Cardellicchio N,Locker D,Leng M,Sivo M,Intini FP,Natile G

    更新日期：1999-10-01 00:00:00

  • Synthesis and biological evaluation of biotin-conjugated anticancer thiosemicarbazones and their iron(III) and copper(II) complexes.

    abstract：:Triapine, the most prominent anticancer drug candidate from the substance class of thiosemicarbazones, was investigated in >30 clinical phase I and II studies. However, the results were rather disappointing against solid tumors, which can be explained (at least partially) due to inefficient delivery to the tumor site....

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2018.10.006

    authors： Kallus S,Uhlik L,van Schoonhoven S,Pelivan K,Berger W,Enyedy ÉA,Hofmann T,Heffeter P,Kowol CR,Keppler BK

    更新日期：2019-01-01 00:00:00

  • A cisplatin slow-release hydrogel drug delivery system based on a formulation of the macrocycle cucurbit[7]uril, gelatin and polyvinyl alcohol.

    abstract：:The anticancer drug cisplatin was encapsulated within the cucurbit[7]uril macrocycle to form the host-guest complex: cisplatin@CB[7]. This was then incorporated into gelatin and 0-4% w/v polyvinyl alcohol (PVA)-based hydrogels as slow release drug delivery vehicles. The hydrogels demonstrated predicable swelling and d...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2014.02.004

    authors： Oun R,Plumb JA,Wheate NJ

    更新日期：2014-05-01 00:00:00

  • Behavior of the potential antitumor V(IV)O complexes formed by flavonoid ligands. 1. Coordination modes and geometry in solution and at the physiological pH.

    abstract：:The coordination modes and geometry assumed in solution by the potent antitumor oxidovanadium(IV) complexes formed by different flavonoids were studied by spectroscopic (Electron Paramagnetic Resonance, EPR) and computational (Density Functional Theory, DFT) methods. A series of bidentate flavonoid ligands (L) with in...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2014.07.007

    authors： Sanna D,Ugone V,Lubinu G,Micera G,Garribba E

    更新日期：2014-11-01 00:00:00

  • Mono- and polynuclear complexes of Pt(II) with polypyridyl ligands. Synthesis, spectroscopic and structural characterization and cytotoxic activity.

    abstract：:An array of poly- and mononuclear complexes of Pt(II) with polypyridyl ligands is reported. The framework complexes [(PtCl(2))(2)(bpp)(2)(micro-PtCl(2))](H(2)O)(2) [bpp=2,3-bis(2-pyridyl)pyrazine], [PtCl(2)(micro-tptz)PtClNCPh]Cl [tptz=2,4,6-tris(2-pyridyl)-1,3,5-triazine], and mononuclear PtCl(2)(NH(2)dpt) [NH(2)dpt=...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2007.06.024

    authors： Rubino S,Portanova P,Albanese A,Calvaruso G,Orecchio S,Fontana G,Stocco GC

    更新日期：2007-10-01 00:00:00

  • Synthesis, characterization and antibacterial studies of a copper(II) levofloxacin ternary complex.

    abstract：:Solution behavior of levofloxacin (lvx) complexes with copper(II) in the presence and absence of phen was studied in aqueous solution, by potentiometry. The results obtained show that under physiological conditions (micromolar concentration range and pH 7.4) only copper(II):lvx:phen ternary complexes are stable. Hence...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2012.02.003

    authors： Sousa I,Claro V,Pereira JL,Amaral AL,Cunha-Silva L,de Castro B,Feio MJ,Pereira E,Gameiro P

    更新日期：2012-05-01 00:00:00

  • Reactivity of iron(II)-bound nitrosyl hydride (HNO, nitroxyl) in aqueous solution.

    abstract：:The reactivity of coordinated nitroxyl (HNO) has been explored with the [Fe(II)(CN)(5)HNO](3-) complex in aqueous medium, pH 6. We discuss essential biorelevant issues as the thermal and photochemical decompositions, the reactivity toward HNO dissociation, the electrochemical behavior, and the reactions with oxidizing...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2012.10.009

    authors： Montenegro AC,Bari SE,Olabe JA

    更新日期：2013-01-01 00:00:00

  • Self-cleavage of p2Sp1 RNA with Mg2+ and non-ionic detergent (Brij 58).

    abstract：:The precursor of an RNA molecule from T4-infected E. coli cells (p2Sp1 RNA) has the capacity to cleave itself at specific positions [(UpA (139-140) and CpA (170-171)], within a putative loop and stem structure. This sequence-specific cleavage requires at least a monovalent cation and non-ionic detergents. We studied t...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0162-0134(00)00164-1

    authors： Hosaka H,Hosono K,Kawai G,Takai K,Takaku H

    更新日期：2000-11-01 00:00:00

  • Copper(II) complexes with peptide fragments encompassing the sequence 122-130 of human doppel protein.

    abstract：:Copper(II) complexes of the peptide fragment (Dpl122-130) encompassing the sequence 122-130 of human doppel protein were characterized by potentiometric, UV-Visible, CD and EPR spectroscopic methods. An analogous peptide, in which the aspartate residue was substituted by an asparagine amino acid, was synthesized in or...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2009.01.017

    authors： Mendola DL,Magrì A,Hansson O,Bonomo RP,Rizzarelli E

    更新日期：2009-05-01 00:00:00

  • Crystal structure based design of functional metal/protein hybrids.

    abstract：:Preparation of metal/protein hybrids is growing into important topics in the field of bioinorganic chemistry. X-ray crystal structure analyses of them provide direct information on unique interactions of metal cations or metal cofactors to understand and design enzymatic functions. In this mini review, the authors foc...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.jinorgbio.2007.06.025

    authors： Ueno T,Yokoi N,Abe S,Watanabe Y

    更新日期：2007-11-01 00:00:00

  • Towards a new attenuating compound: a potentiometric, spectrophotometric and NMR equilibrium study on Fe(III), Al(III) and a new tetradentate mixed bisphosphonate-hydroxypyridinonate ligand.

    abstract：:Coordination properties toward Fe(III) and Al(III) of a mixed bisphosphonate-hydroxypyridinonate ligand are presented. Potentiometric, spectrophotometric and NMR results allowed to conclude that Fe(III) and Al(III) coordination takes place on the pyridinone moiety. The high steric hindrance prevents the possibility of...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2008.01.010

    authors： Crisponi G,Nurchi VM,Pivetta T,Gałezowska J,Gumienna-Kontecka E,Bailly T,Burgada R,Kozłowski H

    更新日期：2008-07-01 00:00:00

  • The systematic study of aluminium speciation in medium concentrated aqueous solutions.

    abstract：:Industrial applications and environmental problems involving the aqueous chemistry of aluminium require an understanding of the speciation of this metal ion at a wide range of concentrations. The formation of polynuclear species is of special interest due to the complexity of the hydrolysis mechanisms and the diversit...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0162-0134(01)00326-9

    authors： Perry CC,Shafran KL

    更新日期：2001-11-01 00:00:00

  • Resonance Raman spectroscopy of Compound II and its decay in Mycobacterium tuberculosis catalase-peroxidase KatG and its isoniazid resistant mutant S315T.

    abstract：:The reaction of Mycobacterium tuberculosis KatG and the mutant KatG(S315T) with two different organic peroxides is studied using resonance Raman spectroscopy. For the first time, an intermediate is observed in a catalase-peroxidase with vibrations that are characteristic of Compound II. The observation of this interme...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2005.03.016

    authors： Kapetanaki SM,Chouchane S,Yu S,Magliozzo RS,Schelvis JP

    更新日期：2005-06-01 00:00:00

  • In vitro antitumor activity of free and nano-encapsulated Na5[PMo10V2O40]·nH2O and its binding properties with ctDNA by using combined spectroscopic methods.

    abstract：:Free and nanosized starch and lipid encapsulated Na5[PMo10V2O40]·nH2O complexes (abbreviated as PMoV, SEP and LEP, respectively) have been prepared and structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, inductively coupled plasma (ICP) analysis, scanning electron microscopy (SEM) and transm...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2015.08.015

    authors： Dianat S,Bordbar AK,Tangestaninejad S,Yadollahi B,Amiri R,Zarkesh-Esfahani SH,Habibi P

    更新日期：2015-11-01 00:00:00

  • Vanadium and insulin: Partners in metabolic regulation.

    abstract：:Since the 1970s, the biological role of vanadium compounds has been discussed as insulin-mimetic or insulin-enhancer agents. The action of vanadium compounds has been investigated to determine how they influence the insulin signaling pathway. Khan and coworkers proposed key proteins for the insulin pathway study, intr...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2020.111094

    authors： Treviño S,Diaz A

    更新日期：2020-07-01 00:00:00

  • Nanosized flower-like ZnO synthesized by a simple hydrothermal method and applied as matrix for horseradish peroxidase immobilization for electro-biosensing.

    abstract：:Nanosized flower-like ZnO was synthesized by a simple hydrothermal method which is a convenient, environment friendly, inexpensive and efficient process. Raman spectroscopy, X-ray diffraction (XRD) and scanning electron microscope (SEM) were used to confirm the material structure and the crystallite microstructure. Th...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.jinorgbio.2005.07.001

    authors： Liu YL,Yang YH,Yang HF,Liu ZM,Shen GL,Yu RQ

    更新日期：2005-10-01 00:00:00

  • The metal complexes of N-confused porphyrin as heme model compounds.

    abstract：:Recently, metal complexes of the isomers and analogs of porphyrin have become important model compounds for heme enzymes and proteins. While the chemistry of metalloporphyrins as heme models still attracts attention, the isomers and analogs of porphyrins provide insight into the biological choice of porphine as the ma...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.jinorgbio.2006.01.016

    authors： Harvey JD,Ziegler CJ

    更新日期：2006-04-01 00:00:00

  • Dependence of divalent metal ions on phosphotransferase activity of osseous plate alkaline phosphatase.

    abstract：:Kinetic evidence for the role of divalent metal ions in the phosphotransferase activity of polidocanol-solubilized alkaline phosphatase from osseous plate is reported. Ethylenediamine tetreacetate, 1,10-phenanthrolin, and Chelex-100 were used to prepare metal-depleted alkaline phosphatase. Except for Chelex-100, eithe...

    journal_title：Journal of inorganic biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0162-0134(96)00159-6

    authors： Ciancaglini P,Pizauro JM,Leone FA

    更新日期：1997-04-01 00:00:00