Self-cleavage of p2Sp1 RNA with Mg2+ and non-ionic detergent (Brij 58).

Abstract:

:The precursor of an RNA molecule from T4-infected E. coli cells (p2Sp1 RNA) has the capacity to cleave itself at specific positions [(UpA (139-140) and CpA (170-171)], within a putative loop and stem structure. This sequence-specific cleavage requires at least a monovalent cation and non-ionic detergents. We studied the self-cleavage reaction of an RNA fragment (GUUUCGUACAAAC) (R1) with the sequence corresponding to the p2Sp1 RNA in the presence of Mg2+ and non-ionic detergents. It requires Mg2+ and is aided by a non-ionic detergent, Brij 58. The cleavage reaction is time, temperature, and pH-dependent. The cleavage occurs at the phosphodiester bond between UpA and CpA on the RNA fragment (GUUUCGUACAAAC) (R1). Furthermore, the maximum of cleavage of R1 occurs at a very low Mg2+ concentration (< or = 5 mM).

journal_name

J Inorg Biochem

authors

Hosaka H,Hosono K,Kawai G,Takai K,Takaku H

doi

10.1016/s0162-0134(00)00164-1

subject

Has Abstract

pub_date

2000-11-01 00:00:00

pages

215-9

issue

1-4

eissn

0162-0134

issn

1873-3344

pii

S0162-0134(00)00164-1

journal_volume

82

pub_type

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