Characterization and cross-protection evaluation of M949_1603 gene deletion Riemerella anatipestifer mutant RA-M1.

abstract：:Corynebacterium glutamicum shows a great potential for the production of the polyamide monomer putrescine (1,4-diaminobutane). Previously, we constructed the putrescine-producing strain PUT1 by deletion of argF, the gene for ornithine transcarbamoylase (OTC), and argR, encoding the L-arginine repressor, combined with ...

journal_title：Applied microbiology and biotechnology

authors： Schneider J,Eberhardt D,Wendisch VF

更新日期：2012-07-01 00:00:00

abstract：:Variable domains of heavy chains of camelid heavy-chain antibodies (VHHs) are known as nanobodies. Nanobodies are approximately 15 kDa in size with high affinity to their antigens. They can be easily manipulated and produced in microorganisms. In this study, an alpaca was immunized with purified green fluorescence pro...

journal_title：Applied microbiology and biotechnology

authors： Fang Z,Cao D,Qiu J

更新日期：2020-06-01 00:00:00

abstract：:The demand of renewable energy sources, i.e. biomass, is steadily increasing worldwide to reduce the need of fossil energy sources. Biomass such as energy crops, woody species, forestry and agricultural residues are the most common renewable energy sources. Due to uneven demand for wood fuel, the material is mostly st...

journal_title：Applied microbiology and biotechnology

authors： Noll M,Jirjis R

更新日期：2012-08-01 00:00:00

abstract：:A hydraulic retention time (HRT) of 4, 6, and 8 h was employed, respectively, in three reactors to study the effects of HRT on granulation with a newly developed fast granulation strategy, i.e., a strategy by combining strong hydraulic selection pressure with high organic loading rate (OLR). Granules with clear bounda...

journal_title：Applied microbiology and biotechnology

authors： Liu YQ,Zhang X,Zhang R,Liu WT,Tay JH

更新日期：2016-01-01 00:00:00

abstract：:The upstream region of the cellobiohydrolase gene cbhA of Clostridium thermocellum F7 was sequenced. It was found that this region contains the previously sequenced gene celK encoding an enzyme closely related to CbhA (cellulosomal subunit S3). The presence of a putative transcription terminator in the 524-bp intergen...

journal_title：Applied microbiology and biotechnology

authors： Zverlov VV,Velikodvorskaya GA,Schwarz WH,Kellermann J,Staudenbauer WL

更新日期：1999-06-01 00:00:00

abstract：:A novel biosuperabsorbent protein hydrogel was prepared from protein-rich alcoholic-alkali soluble parts of zygomycete Rhizomucor pusillus biomass. The fungal protein content was 46.8%, and the lipid content was 13.1%. Extraction of protein from this microorganism through the method applied prevents protein decomposit...

journal_title：Applied microbiology and biotechnology

authors： Majdejabbari S,Barghi H,Taherzadeh MJ

更新日期：2011-12-01 00:00:00

abstract：:A biosynthetic pathway for the production of (S)-3-hydroxybutyric acid (S3HB) from glucose was established in recombinant Escherichia coli by introducing the beta-ketothiolase gene from Ralstonia eutropha H16, the (S)-3-hydroxybutyryl-CoA dehydrogenase gene from R. eutropha H16, or Clostridium acetobutylicum ATCC824, ...

journal_title：Applied microbiology and biotechnology

authors： Lee SH,Park SJ,Lee SY,Hong SH

更新日期：2008-06-01 00:00:00

abstract：:C (2) to C (4) chloroalkanes have been used for a wide range of industrial applications. Consequently, numerous leaks to the environment have occurred. It is generally observed that the lower chlorinated members of the group, containing 1-3 chlorine atoms, accumulate in environments where reductive conditions prevail....

journal_title：Applied microbiology and biotechnology

authors： De Wildeman S,Verstraete W

更新日期：2003-04-01 00:00:00

abstract：:Biofilms are widely used in wastewater treatment for their particular enhancement of nitrogen removal and other significant advantages. In this study, the diversity and potential functions of nitrogen removal bacteria in suspended activated sludge (AS) and biofilm of a full-scale hybrid reactor were uncovered by metag...

journal_title：Applied microbiology and biotechnology

authors： Chao Y,Mao Y,Yu K,Zhang T

更新日期：2016-09-01 00:00:00

abstract：:The paper describes the synthesis of the phosphorylcholine-binding miniantibody McPC603scFvDhl x in cell-wall-less L-form strains of Escherichia coli and Proteus mirabilis. Cells of these strains were transformed with the plasmid pACK02scKan, carrying the miniantibody (miniAb) coding sequence under the control of the ...

journal_title：Applied microbiology and biotechnology

authors： Kujau MJ,Hoischen C,Riesenberg D,Gumpert J

更新日期：1998-01-01 00:00:00

abstract：:We report here the creation of a modular, plasmid-based protein expression system utilizing elements of the native Rhodobacter puf promoter in a BioBrick(TM)-based vector system with DsRed encoding a red fluorescent reporter protein. A suite of truncations of the puf promoter were made to assess the influence of diffe...

journal_title：Applied microbiology and biotechnology

authors： Tikh IB,Held M,Schmidt-Dannert C

更新日期：2014-04-01 00:00:00

abstract：:Poly(hydroxyalkanoates) (PHAs) constitute biodegradable polyesters and are considered among the most promising candidates to replace common petrochemical plastics in various applications. To date, all commercial processes for PHA production employ microbial discontinuous fed-batch fermentations. These processes featur...

journal_title：Applied microbiology and biotechnology

authors： Atlić A,Koller M,Scherzer D,Kutschera C,Grillo-Fernandes E,Horvat P,Chiellini E,Braunegg G

更新日期：2011-07-01 00:00:00

abstract：:2-Phenylethanol (2-PE) is an important flavor compound but also impairs cell growth severely, which in turn blocks its bioproduction. However, the molecular mechanism of 2-PE tolerance is unclear. In this study, a superb 2-PE stress-tolerant and producing yeast, Candida glycerinogenes, was selected to uncover the unde...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Zhang Z,Lu X,Zong H,Zhuge B

更新日期：2020-05-01 00:00:00

abstract：:The Baeyer-Villiger monooxygenase (BVMO) BmoF1 from Pseudomonas fluorescens DSM 50106 was shown before to enantioselectively oxidize different 4-hydroxy-2-ketones to the corresponding hydroxyalkyl acetates, being the first example of a BVMO-catalyzed kinetic resolution of aliphatic acyclic ketones. However, the wild-t...

journal_title：Applied microbiology and biotechnology

authors： Kirschner A,Bornscheuer UT

更新日期：2008-12-01 00:00:00

abstract：:Furfural is one of the major inhibitors generated during sugar production from cellulosic materials and, as an aldehyde, inhibits various cellular activities of microorganisms used, leading to prolonged lag time during ethanologenic fermentation. Since Saccharomyces cerevisiae strains tolerant to furfural are of great...

journal_title：Applied microbiology and biotechnology

authors： Kim HS,Kim NR,Kim W,Choi W

更新日期：2012-07-01 00:00:00

abstract：:Fat, oil and, grease (FOG) deposits are one primary cause of sanitary sewer overflows (SSOs). While numerous studies have examined the formation of FOG deposits in sewer pipes, little is known about their biodegradation under sewer environments. In this study, FOG deposit biodegradation potential was determined by stu...

journal_title：Applied microbiology and biotechnology

authors： He X,Zhang Q,Cooney MJ,Yan T

更新日期：2015-07-01 00:00:00

abstract：:In this study, the kinetic behaviors between n-butyl acetate and composite bead were investigated. Both microbial growth rate and biochemical reaction rate would be inhibited with increasing average inlet concentration. The order of the inhibitive effect, which resulted from increased average inlet concentration for f...

journal_title：Applied microbiology and biotechnology

authors： Chan WC,Hwang ID

更新日期：2006-11-01 00:00:00

abstract：:Microbially induced CaCO3 precipitation (MICP) can give concrete self-healing properties. MICP agents are typically bacterial endospores which are coated into shelled granules, infused into expanded clay, or embedded into superabsorbent polymer (SAP). When small cracks appear in the cured concrete, the encapsulation i...

journal_title：Applied microbiology and biotechnology

authors： Nielsen SD,Koren K,Löbmann K,Hinge M,Scoma A,Kjeldsen KU,Røy H

更新日期：2020-01-01 00:00:00

abstract：:Fatty acid desaturases play vital roles in the synthesis of unsaturated fatty acids. In this study, Δ12 and Δ12/Δ15 fatty acid desaturases of the oleaginous yeast Lipomyces starkeyi, termed LsFad2 and LsFad3, respectively, were identified and characterized. Saccharomyces cerevisiae expressing LsFAD2 converted oleic ac...

journal_title：Applied microbiology and biotechnology

authors： Matsuzawa T,Maehara T,Kamisaka Y,Ara S,Takaku H,Yaoi K

更新日期：2018-10-01 00:00:00

abstract：:Aspergillus oryzae is a fungus used extensively in the fermentation industry. We constructed cDNA microarrays comprising 2,070 highly expressed cDNAs selected from the approximately 6,000 non-redundant expressed sequence tags (ESTs) in the A. oryzae EST database (http://www.aist.go.jp/RIODB/ffdb/index.html). Using the...

journal_title：Applied microbiology and biotechnology

authors： Maeda H,Sano M,Maruyama Y,Tanno T,Akao T,Totsuka Y,Endo M,Sakurada R,Yamagata Y,Machida M,Akita O,Hasegawa F,Abe K,Gomi K,Nakajima T,Iguchi Y

更新日期：2004-07-01 00:00:00

abstract：:α-Galacto-oligosaccharides (α-GOS) are produced by transgalactosylation reactions of α-galactosidase (α-Gal) or by conversion of raffinose family oligosaccharides by levansucrase. Similarly to β-GOS, α-GOS have the potential to mimic glycan receptors on eukaryotic cells and act as molecular decoys to prevent bacterial...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Black BA,Curtis JM,Gänzle MG

更新日期：2014-03-01 00:00:00

abstract：:Although the de novo biosynthetic mechanism of 3-hydroxypropionic acid (3-HP) in glycerol-fermenting microorganisms is still unclear, the propanediol utilization protein (PduP) of Lactobacillus species has been suggested to be a key enzyme in this regard. To verify this hypothesis, a pduP gene from Lactobacillus reute...

journal_title：Applied microbiology and biotechnology

authors： Luo LH,Seo JW,Baek JO,Oh BR,Heo SY,Hong WK,Kim DH,Kim CH

更新日期：2011-02-01 00:00:00

abstract：:Studies have shown that external stress induces biofilm formation, but the underlying details are not clearly understood. This study investigates the changes in cell surface properties leading to increase in biofilm formation by Staphylococcus aureus and Pseudomonas aeruginosa in the presence of streptomycin. Bacteria...

journal_title：Applied microbiology and biotechnology

authors： Kumar A,Ting YP

更新日期：2016-10-01 00:00:00

abstract：:Immobilization of the enzyme benefits the catalytic industry a lot. The gram-positive enhancer matrix (GEM) particles could purify and immobilize the recombinant α-amylase in one step without changing the enzymatic character. The enzyme immobilized by GEM particles exhibited good reusability and storage stability. The...

journal_title：Applied microbiology and biotechnology

authors： Zhao F,Song Q,Wang B,Han Y,Zhou Z

更新日期：2020-01-01 00:00:00

abstract：:Drug-resistant bacteria are becoming an increasingly widespread problem in the clinical setting. The current pipeline of antibiotics cannot provide satisfactory options for clinicians, which brought increasing attention to the development and application of non-traditional antimicrobial substances as alternatives. Met...

journal_title：Applied microbiology and biotechnology

authors： Ye Q,Chen W,Huang H,Tang Y,Wang W,Meng F,Wang H,Zheng Y

更新日期：2020-06-01 00:00:00

abstract：:Heterorhabditis bacteriophora is used in biological control of soil-borne insect pests in horticulture and turf. Mass production is carried out in monoxenic liquid cultures pre-incubated with the symbiont of the nematodes, the bacterium Photorhabdus luminescens, before nematode dauer juveniles (DJ) are inoculated. As ...

journal_title：Applied microbiology and biotechnology

authors： Johnigk SA,Ecke F,Poehling M,Ehlers RU

更新日期：2004-06-01 00:00:00

abstract：:In the Funding section, the following statement is missing: The MACS cohort study was supported by the NIH, National Institute of Allergy and Infectious Diseases grant U01-AI35041. ...

journal_title：Applied microbiology and biotechnology

authors： Palma ML,Garcia-Bates TM,Martins FS,Douradinha B

更新日期：2019-07-01 00:00:00

abstract：:Increasing evidence suggests that maintenance of homeostasis between gut microbiota and host plays an important role in human health. Many diseases, such as those affecting the liver, have been linked to imbalances in gut microbial communities. However, it is not clear whether an imbalance in gut microbiota promotes t...

journal_title：Applied microbiology and biotechnology

authors： Hu X,Wang T,Liang S,Li W,Wu X,Jin F

更新日期：2015-11-01 00:00:00

abstract：:Expression of recombinant proteins in Escherichia coli often requires use of inducible promoters to shorten the lag phase and improve protein productivity and final protein titer. Synthetic molecules that cannot be metabolized by E. coli, such as isopropyl thiogalactopyranoside (IPTG), have been frequently used to tri...

journal_title：Applied microbiology and biotechnology

authors： Anilionyte O,Liang H,Ma X,Yang L,Zhou K

更新日期：2018-08-01 00:00:00

abstract：:Two bacterial strains, Ralstonia sp. LD35 and Pseudomonas putida DSM 1868, were assayed for their ability to degrade the monocyclic aromatic compounds commonly found in olive mill wastewaters (OMWs). The goal was to study the possibility of employing the two strains in the removal of these recalcitrant and toxic compo...

journal_title：Applied microbiology and biotechnology

authors： Di Gioia D,Fava F,Bertin L,Marchetti L

更新日期：2001-05-01 00:00:00