Abstract:
:The overproduction of enzymes was performed by manipulating the medium components. In our study, solvent-tolerant thermophilic lipase-producing Bacillus coagulans was isolated from soil samples and a stepwise optimization strategy was employed to increase the lipase production using coconut oil cake basal medium. In the first step, the influence of pH, temperature, carbon source, nitrogen source and inducers on lipase activity was investigated by the One-Factor-At-A-Time (OFAT) method. In the second step, the three significant factors resulted from OFAT were optimized by the statistical approach (CCD).The optimum values of olive oil (0.5%), Tween 80 (0.6%) and FeSO4 (0.05%) was found to be responsible for a 3.2-fold increase in the lipase production identified by Central Composite Design.
journal_name
Biocontrol Scijournal_title
Biocontrol scienceauthors
Gowthami P,Muthukumar K,Velan Mdoi
10.4265/bio.20.125subject
Has Abstractpub_date
2015-01-01 00:00:00pages
125-33issue
2eissn
1342-4815issn
1884-0205journal_volume
20pub_type
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