Utilization of coconut oil cake for the production of lipase using Bacillus coagulans VKL1.

Abstract:

:The overproduction of enzymes was performed by manipulating the medium components. In our study, solvent-tolerant thermophilic lipase-producing Bacillus coagulans was isolated from soil samples and a stepwise optimization strategy was employed to increase the lipase production using coconut oil cake basal medium. In the first step, the influence of pH, temperature, carbon source, nitrogen source and inducers on lipase activity was investigated by the One-Factor-At-A-Time (OFAT) method. In the second step, the three significant factors resulted from OFAT were optimized by the statistical approach (CCD).The optimum values of olive oil (0.5%), Tween 80 (0.6%) and FeSO4 (0.05%) was found to be responsible for a 3.2-fold increase in the lipase production identified by Central Composite Design.

journal_name

Biocontrol Sci

journal_title

Biocontrol science

authors

Gowthami P,Muthukumar K,Velan M

doi

10.4265/bio.20.125

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

125-33

issue

2

eissn

1342-4815

issn

1884-0205

journal_volume

20

pub_type

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