Abstract:
:The simplex real-time PCR assays based on the TaqMan probe and SYBR green I, which target cereulide synthetase genes (ces genes) were used for rapidly, reliably and sensitively identifying the emetic strains from among Bacillus cereus strains isolated from different sources. Only the emetic strains showed positive reactions to the real-time PCR assays, but all examined strains of diarrheal B. cereus, other Bacillus species and other gram positive and negative bacteria gave negative results. The final identification of emetic B. cereus was possible within 1 to 1.5 h in both simplex real-time PCR procedures. The detection limit of emetic strains in food was suggested to be 10(4)-10(5) cfu/g. Both simplex real-time PCR assays were found to be rapid, sensitive and reliable diagnostic tools that complement the different cellular, immunological and chemical detection methods for cereulide- producing B. cereus, even if a relatively expensive device is required for the assays.
journal_name
Biocontrol Scijournal_title
Biocontrol scienceauthors
Ueda S,Yamaguchi M,Iwase M,Kuwabara Ydoi
10.4265/bio.18.227subject
Has Abstractpub_date
2013-01-01 00:00:00pages
227-32issue
4eissn
1342-4815issn
1884-0205pii
DN/JST.JSTAGE/bio/18.227journal_volume
18pub_type
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