Antifreeze Activity of Xylomannan from the Mycelium and Fruit Body of Flammulina velutipes.

Abstract:

:An identified class of antifreeze, a xylomannan-based thermal hysteresis (TH)-producing glycolipid, has been discovered from diverse taxa, including plants, insects, and amphibians. We isolated xylomannan from the mycelium and fruit body of the basidiomycete Flammulina velutipes using successive hot extraction with water, 2% and 25% aqueous KOH, and gel filtration chromatography. The xylomannan from the fruit body had a recrystallization inhibiting (RI) activity (RI=0.44) at 0.5 mg/mL. The dried weight yield of the fruit body (7.7×10(-2)%, w/w) was higher than that of the mycelium. Although the purified xylomannan from both soures were composed of mannose and xylose in a 2 : 1 molar ratio, the molecular weight of the xylomannan from the mycelium and fruit body was 320,000 and 240,000, respectively. The RI activity of mycelial xylomannan was higher than that from the fruit body (RI=0.57) at 45 µg/mL. Although this RI activity was able to remain constant after exposure to various conditions, we confirmed that the decrease of RI activity was stimulated by the decrease of molecular weight that was caused by heating during the alkaline condition. The survival rate of the CHO cells at -20℃ for two days increased to 97% due to the addition of 20 µg/mL of purified xylomannan. This was the first report to indicate that xylomannan from the mycelium of Flammulina velutipes had a high level of ice recrystallization inhibiting activity like antifreeze proteins from plants and had rhe potential to become a new material for cell storage.

journal_name

Biocontrol Sci

journal_title

Biocontrol science

authors

Kawahara H,Matsuda Y,Sakaguchi T,Arai N,Koide Y

doi

10.4265/bio.21.153

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

153-9

issue

3

eissn

1342-4815

issn

1884-0205

journal_volume

21

pub_type

杂志文章
  • Development of a small-sized generator of ozonated water using an electro-conductive diamond electrode.

    abstract::A small-sized generator of ozonated water was developed using an electro-conductive diamond. We studied the optimum conditions for producing ozonated water. As a result, we developed a small-sized generator of ozonated water driven by a dry-cell for use in the average household. This generator was easily able to produ...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.13.119

    authors: Sekido K,Kitaori N

    更新日期:2008-12-01 00:00:00

  • Genetic Identification of Dematiaceous Fungi Isolated from Washing Machine in Japan, and Considering of Fungal Removal Methods.

    abstract::In 2017, 2 household washing machines which were used at a house in Saitama prefecture were tested for fungal flora sampling. The fungi were identified in detail via genetic analysis of the ITS region. The number of fungi which were isolated were 8.7×106~6.1×107 cfu/100cm2 and dematiaceous fungi such as Ochroconis mus...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.24.89

    authors: Hashimoto K,Fujii H,Kawakami Y

    更新日期:2019-01-01 00:00:00

  • Development of a density slicer for the simple collection of respective density layers after stepwise density gradient centrifugation.

    abstract::Density gradient centrifugation (DGC) is useful for the separation of living microbial cells from food samples that are not filterable. After DGC, however, careful operation is necessary to collect each density layer. For a simple and reproducible collection after DGC, we have developed a seamless operation system com...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.15.39

    authors: Araki E,Matsuzaki T,Sekita T,Saito M,Matsuoka H

    更新日期:2010-06-01 00:00:00

  • Solutions for biological indicator problems from a quality assurance viewpoint.

    abstract::A biological indicator (BI) is a useful product to confirm sterility assurance after a sterilization procedure. In ISO 11138 and 14161, the concrete procedures regarding the use of BI are described for BI manufacturers and users, respectively. There are several problems involving BI use which must be addressed. These ...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.12.77

    authors: Royalty-Hann W

    更新日期:2007-06-01 00:00:00

  • Comparison of the Filter Efficiency of Medical Nonwoven Fabrics against Three Different Microbe Aerosols.

    abstract:: Exact evaluation of the performance of surgical masks and biohazard protective clothing materials against pathogens is important because it can provide helpful information that healthcare workers can use to select suitable materials to reduce infection risk. Currently, to evaluate the protective performance of nonwov...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.23.61

    authors: Shimasaki N,Okaue A,Kikuno R,Shinohara K

    更新日期:2018-01-01 00:00:00

  • Effect of magnesium peroxide biostimulation of fish feed-loaded marine sediments on changes in the bacterial community.

    abstract::The effect of an oxygen-releasing compound (ORC) magnesium peroxide (MgO(2)) on the changes in the bacterial community in organically polluted sediment of aquaculture farms was tested in a microcosm experiment. The sediment, to which fish feed was added, was treated with 1% or 5% MgO(2). The addition of fish feed indu...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.18.41

    authors: Santander-De Leon SM,Okunishi S,Kihira M,Nakano M,Nuñal SN,Hidaka M,Yoshikawa T,Maeda H

    更新日期:2013-01-01 00:00:00

  • Antimicrobial action of a unique phosphorus-adsorbent additive for resin, and the mechanism of its antimicrobial effect.

    abstract::We found that an additive for a resin, which was comprised of collagen and aluminum (Al), showed a strong and stable antibacterial effect against various bacterium under certain conditions. We tried to clarify its mechanism of action, and investigated optimum conditions for its effects. This additive (Al cross-linked ...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.17.37

    authors: Ohmoto T,Yamashita K,Moriyoshi K,Yamanaka H,Koma D,Kawano H,Sakai K

    更新日期:2012-03-01 00:00:00

  • Genotyping and chlorine-resistance of Methylobacterium aquaticum isolated from water samples in Japan.

    abstract::For microbial ecological analysis, 14 strains of Methylobacterium aquaticum isolated from water samples were subjected to clustering analysis on the basis of ribotyping and RAPD-PCR tests. The ribopatterns after digestion with EcoRI obtained from 14 strains of M. aquaticum were used to divide the strains into two grou...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.16.103

    authors: Furuhata K,Banzai AU,Kawakami Y,Ishizaki N,Yoshida Y,Goto K,Fukuyama M

    更新日期:2011-09-01 00:00:00

  • Antimicrobial activity of isothiocyanates (ITCs) extracted from horseradish (Armoracia rusticana) root against oral microorganisms.

    abstract::The antimicrobial activity of isothiocyanates (ITCs) extracted from horseradish root was investigated against oral microorganisms: 6 strains of facultative anaerobic bacteria, Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, Staphylococcus aureus, Enterococcus faecalis and Aggregatibacter actinomycet...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.18.163

    authors: Park HW,Choi KD,Shin IS

    更新日期:2013-01-01 00:00:00

  • Utilization of coconut oil cake for the production of lipase using Bacillus coagulans VKL1.

    abstract::The overproduction of enzymes was performed by manipulating the medium components. In our study, solvent-tolerant thermophilic lipase-producing Bacillus coagulans was isolated from soil samples and a stepwise optimization strategy was employed to increase the lipase production using coconut oil cake basal medium. In t...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.20.125

    authors: Gowthami P,Muthukumar K,Velan M

    更新日期:2015-01-01 00:00:00

  • In Vitro Evaluation of the Biocompatibility of Newly Synthesized Bis-Quaternary Ammonium Compounds with Spacer Structures Derived from Pentaerythritol or Hydroquinone.

    abstract:: With the object of developing new biocides milder for human use than the current antiseptics, we synthesized a series of bis-quaternary ammonium compounds (bis-QACs). The antimicrobial activity of the newly synthesized bis-QACs and common biocides used as antiseptics was compared by examining minimum inhibitory conce...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.21.231

    authors: Yamamoto M,Takami T,Matsumura R,Dorofeev A,Hirata Y,Nagamune H

    更新日期:2016-01-01 00:00:00

  • Effects of Diluents, Saliva and Other Organics on the Microbicidal Activity of Cetylpyridinium Chloride and Povidone-iodine.

    abstract::Povidone-iodine (PVP-I) is used for infection control and preoperative sterilization of the oral and pharyngeal regions. Marketed preparations containing cetylpyridinium chloride (CPC) are used to inhibit growth of oral bacteria. We conducted an in vitro study of the sterilizing effects of these microbicides on 10 ora...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.25.45

    authors: Urakawa R,Inubushi J,Tobata H,Eguchi T

    更新日期:2020-01-01 00:00:00

  • Cloning and analysis of the ggpS gene from Cyanobacteria Arthrospira spp. involved in the synthesis of an osmolyte glucosylglycerol.

    abstract::The genus Arthrospira is a nonheterocystous filamentous cyanobacterium inhabiting diverse environments including those of high salinity. In the present study, Arthrospira strains were isolated from freshwater and brackish lakes in Myanmar, and their osmoprotective adaptation was investigated as it was for the genus Sy...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.16.55

    authors: Yoshikawa T,Ikeda Y,Sakata T,Maeda H

    更新日期:2011-06-01 00:00:00

  • Thermal death of Bacillus subtilis spores in oil-water systems.

    abstract::The thermal death of the spores of Bacillus subtilis 168 in oil-water systems including emulsions and separated layers consisting of phosphate buffer and soybean oil or n-hexadecane was investigated. The resultant survivor curve consisted of two phases, an initial rapid reduction followed by a slow reduction, possibly...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.15.27

    authors: Shigemoto M,Nakagawa K,Sakamoto JJ,Tsuchidoi T

    更新日期:2010-03-01 00:00:00

  • Antifungal Activity of Diglycerin Ester of Fatty Acids against Yeasts and Its Comparison with Those of Sucrose Monopalmitate and Sodium Benzoate.

    abstract::The antifungal activities of diglycerin monoester of fatty acids (DGCs), which have been employed as food emulsifiers, were examined against three yeasts, Saccharomyces cerevisiae, Candida albicans and Candida utilis and were compared with those of sucrose monoester of palmitic acid (SC16) as another type of emulsifie...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.21.123

    authors: Shimazaki A,Sakamoto JJ,Furuta M,Tsuchido T

    更新日期:2016-01-01 00:00:00

  • Kinetic analysis of the antifungal activity of heated scallop-shell powder against Trichophyton and its possible application to the treatment of dermatophytosis.

    abstract::The antifungal activity of scallop-shell powder heated at 1000 degrees C for 1 h against Trichophyton was kinetically investigated and the possibility of applying the powder to the treatment of dermatophytosis was examined. The death rate of T. mentagrophytes NBRC5466 in the heated shell powder slurry increased with p...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.11.125

    authors: Sawai J,Shiga H

    更新日期:2006-09-01 00:00:00

  • Binding sites on tau proteins as components for antimicrobial peptides.

    abstract::To design new antimicrobial peptides, we have focused on various proteins which are not essential for self-defense but carry important responsibilities for biosystems. Previously, we reported that highly efficient antimicrobial properties or antiviral properties are inherent in the nuclear translocation signals and bi...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.13.49

    authors: Kobayashi N,Masuda J,Kudoh J,Shimizu N,Yoshida T

    更新日期:2008-06-01 00:00:00

  • Effect of polyphenols on reactive oxygen species production and cell growth of human dermal fibroblasts after irradiation with ultraviolet-A light.

    abstract::Ultraviolet-A (UV-A) can damage microbes by generating reactive oxygen species (ROS), singlet oxygen, superoxides, hydrogen peroxide and hydroxyl radicals. These species readily react with lipids, proteins, DNA and other constituents of cells, leading to oxidative deterioration and the eventual death of the microbe. H...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.20.27

    authors: Shirai A,Onitsuka M,Maseda H,Omasa T

    更新日期:2015-01-01 00:00:00

  • The mechanism of the bactericidal activity of hinokitiol.

    abstract::Hinokitiol, the major component of Aomori Hiba (Thujopsis dolabrata Sieb. et Zucc. var. hondai M(AKINO)) has already been found to show strong antibacterial activity. In this study, its mechanism of action was examined using Escherichia coli IFO 3301 and Staphylococcus aureus IFO 12732. As a result, it was suggested t...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.12.101

    authors: Morita Y,Sakagami Y,Okabe T,Ohe T,Inamori Y,Ishida N

    更新日期:2007-09-01 00:00:00

  • Antifungal activities of bronopol and 2-methyl-4-isothiazolin-3-one (MT) against saprolegnia.

    abstract::This study compared the antifungal activities of bronopol and 2-methyl-4-isothiazolin-3-one (MT) against members of the genus Saprolegnia in the family saprolegniaceae, which have the ability to infect fish. The minimum inhibitory concentration of bronopol was 100-200 mg/l but for MT it was 31 mg/l. Concerning fungici...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.12.145

    authors: Oono H,Hatai K

    更新日期:2007-12-01 00:00:00

  • Lethality of shock pressures to a marine Vibrio sp. isolated from a ship's ballast water.

    abstract::The lethal effects of shock pressure treatment on suspended Vibrio sp. cells were examined. Lethality of shock pressures to the Vibrio sp. cells increased with the increase in the values of maximum shock pressures generated in the cell suspension. When the value was around 114 MPa, the total number of colony-forming c...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.11.159

    authors: Mimura H,Abe A,Katakura R,Kawasaki H,Yoshida K,Ishida H

    更新日期:2006-12-01 00:00:00

  • Isolation of Pseudomonas aeruginosa from cockroaches Captured in hospitals in Japan, and their antibiotic susceptibility.

    abstract::Pseudomonas aeruginosa strains were isolated from 45 of 370 (12.2%) cockroaches captured in hospitals. By cockroach species, the bacterial strains were isolated from 39 of 181 (21.5%) Periplaneta fuliginosa and 6 of 183 (3.3%) Blattella germanica, showing a significant difference (p<0.01). Many P. aeruginosa-carrying ...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.14.155

    authors: Saitou K,Furuhata K,Kawakami Y,Fukuyama M

    更新日期:2009-12-01 00:00:00

  • Fate of Escherichia coli O157 Cells Inoculated into Lightly Pickled Chinese Cabbage during Processing, Storage and Incubation in Artificial Gastric Juice.

    abstract::Fate of Escherichia coli O157 cells was evaluated when inoculated into each step after production of lightly pickled Chinese cabbage. The efficacy of surface sterilization by 100 mg/L of chlorine water for 10 min on raw leaves (6.0 log CFU/g) was 2.2 log CFU/g reduction. No meaningful change of the population of E. co...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.21.51

    authors: Inatsu Y,Ohata Y,Ananchaipattana C,Latiful Bari M,Hosotani Y,Kawasaki S

    更新日期:2016-01-01 00:00:00

  • Characteristics of Biofilms Formed by Co-Culture of Listeria monocytogenes with Pseudomonas aeruginosa at Low Temperatures and Their Sensitivity to Antibacterial Substances.

    abstract::We assessed the properties of biofilms (BFs) formed by mono- and co-cultures of Listeria monocytogenes and Pseudomonas aeruginosa (L+P-BF) at low temperatures and examined their sensitivity to several antibacterial substances. L. monocytogenes viable counts comprised only 1-10% of total L+P-BF viable counts at 10℃ and...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.23.107

    authors: Yamakawa T,Tomita K,Sawai J

    更新日期:2018-01-01 00:00:00

  • In vitro Anti-Influenza Virus Activity of Agaricus brasiliensis KA21.

    abstract:: Agaricus is known to have immunostimulatory and anti-tumor effects. However, the antiviral effects of Agaricus have not yet been examined. In the present study, the antiviral effects of an extract of Agaricus brasiliensis KA21 (AE) on the H1N1 influenza virus (PR8 strain) were investigated. The anti-influenza virus e...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.22.171

    authors: Eguchi N,Fujino K,Thanasut K,Taharaguchi M,Motoi M,Motoi A,Oonaka K,Taharaguchi S

    更新日期:2017-01-01 00:00:00

  • Efficacy and Effectiveness of Showerheads Attached with Point-of-use (POU) Filter Capsules in Preventing Waterborne Diseases in a Japanese Hospital.

    abstract::Tap water contamination is a growing concern in healthcare facilities, and despite chlorination, tap water in these facilities contains several pathogenic microorganisms causing healthcare-associated waterborne infections or nosocomial outbreaks. Shower units are particularly prone to contamination as they are conduci...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.25.223

    authors: Sasahara T,Ogawa M,Fujimura I,Ae R,Kosami K,Morisawa Y

    更新日期:2020-01-01 00:00:00

  • Environmental Impact of Tributyltin-Resistant Marine Bacteria in the Indigenous Microbial Population of Tributyltin-Polluted Surface Sediments.

    abstract:: We compared the TBT-resistant ability of resting cells prepared from isolates that formed colonies on nutrient agar plates containing 100 µM tributyltin (TBT) chloride, such as Photobacterium sp. TKY1, Halomonas sp. TKY2, and Photobacterium sp. NGY1, with those from taxonomically similar type strains. Photobacterium ...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.22.89

    authors: Mimura H,Yagi M,Yoshida K

    更新日期:2017-01-01 00:00:00

  • Rapid detection of microbes in the dialysis solution by the microcolony fluorescence staining method (Millflex quantum).

    abstract::A chemiluminescence system, Milliflex Quantum (MFQ), to detect microcolonies, has been used in the pharmaceutical field. In this study, we investigated aquatic bacteria in hemodialysis solutions sampled from bioburden areas in 4 dialysis faculties. Using MFQ, microcolonies could be detected after a short incubation pe...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.19.57

    authors: Osono E,Kobayashi E,Inoue Y,Honda K,Kumagai T,Negishi H,Okamatsu K,Ichimura K,Kamano C,Suzuki F,Norose Y,Takahashi M,Takaku S,Fujioka N,Hayama N,Takizawa H

    更新日期:2014-01-01 00:00:00

  • Accurate Enumeration of Aspergillus brasiliensis in Hair Color and Mascara by Time-Lapse Shadow Image Analysis.

    abstract::The growth of black mold (Aspergillus brasiliensis) in black-colored samples such as hair color and mascara was measured with an automatic count system based on time-lapse shadow image analysis (TSIA). A. brasiliensis suspended in a lecithin and polysorbate (LP) solution of each sample (hair color or mascara) was spre...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.20.281

    authors: Ogawa H,Matsuoka H,Saito M

    更新日期:2015-01-01 00:00:00

  • Effects of UV Irradiation on Penicillium Strains Isolated from a Bread Plant and the Application to Bakery Products.

    abstract::We tested treatement with UV irradiation for controlling the growth of bread mold. First, we analyzed the sterilizing effect of a dose of approximately 25 mJ/cm2 radiation on nine Penicillium and two Talaromyces strains that were isolated from a bread-manufacturing plant. The P. chermesinum and P. paneum strains were ...

    journal_title:Biocontrol science

    pub_type: 杂志文章

    doi:10.4265/bio.24.179

    authors: Kawaguchi M,Kani A,Takatori K

    更新日期:2019-01-01 00:00:00