Construction and characterization of a Saccharomyces cerevisiae strain (RIT4376) expressing hepatitis B surface antigen.

Abstract:

:A host/vector system suitable for large-scale production of HBsAg has been constructed and optimized in terms of the expression plasmid and yeast host strain in order to permit fermentation to very high cell densities. The final expression plasmid contains the coding sequence of the major HBsAg protein (P24) flanked by the promoter sequences from a glycolytic gene and by the transcription-termination region of the ARG3 gene. The host/vector system was found to be genetically stable under large-scale fermentation conditions as demonstrated by nucleotide sequencing and restriction mapping experiments. The P24 protein is recovered from yeast as particles whose physiochemical properties are very similar to those of plasma-derived HBsAg.

journal_name

Postgrad Med J

authors

Harford N,Cabezon T,Colau B,Delisse AM,Rutgers T,De Wilde M

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

65-70

eissn

0032-5473

issn

1469-0756

journal_volume

63 Suppl 2

pub_type

杂志文章
  • Tissue plasminogen activator in the treatment of superior vena caval thrombosis associated with parenteral nutrition.

    abstract::Two patients, one of whom was pregnant, developed superior vena caval thrombosis while receiving central parenteral nutrition. They were successfully treated with recombinant tissue plasminogen activator (t-PA). ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.66.775.398

    authors: Barclay GR,Allen K,Pennington CR

    更新日期:1990-05-01 00:00:00

  • Transient atrial fibrillation in hypertensive patients with thiazide induced hypokalaemia.

    abstract::During the previous 34 months, 3 hypertensive patients on long-term thiazide therapy were admitted to the medical department, Mubarak Hospital, Kuwait, with atrial fibrillation (AF) and hypokalaemia. They received potassium chloride by intravenous infusion, followed by oral therapy with reversion to sinus rhythm. Ther...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.62.734.1125

    authors: Emara MK,Saadet AM

    更新日期:1986-12-01 00:00:00

  • To bed or not to bed: the sleep question?

    abstract:BACKGROUND:Sleep deprivation and fatigue from long-shift work impacts doctors' personal safety, inhibits cognitive performance and risks clinical error. The aim of this study was to assess the sleep quality of surgical trainees participating in European Working Time Directive-compliant training rotations within a UK de...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/postgradmedj-2018-135795

    authors: Brown C,Abdelrahman T,Lewis W,Pollitt J,Egan R,members of the Welsh Surgical Research Initiative.

    更新日期:2020-09-01 00:00:00

  • Immunogenicity of a recombinant DNA hepatitis B vaccine in neonates.

    abstract::Infants of HBsAg-positive mothers (Group I) as well as those born to women without HBV markers (Group II) were vaccinated with a 10 micrograms dose of a recombinant DNA hepatitis B vaccine within 24 hours after birth according to a 0, 1, and 2 month schedule, with a booster dose planned 12 months later. Vaccination re...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:

    authors: Meheus A,Alisjahbana A,Vranckx R,Sukadi A,Usman A,Ngantung W,Sugita E,Safary A,André F,Reniers J

    更新日期:1987-01-01 00:00:00

  • Sheehan's syndrome presenting with type III hyperlipoproteinaemia.

    abstract::A patient is described in whom the presenting feature of hypopituitarism was the development of palmar xanthomata associated with type III hyperlipoproteinaemia. Treatment of her secondary hypothyroidism with thyroxine caused resolution of the xanthomata and hyperlipidaemia, but the underlying compositional abnormalit...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.63.746.1099

    authors: Carr D,Thornes HM,Rutter AC,Finney RD,Turner PR

    更新日期:1987-12-01 00:00:00

  • Myocardial abscess and sternal osteomyelitis following myocardial infarction and resuscitation.

    abstract::A 58 year old man presented with an anterior myocardial infarction requiring repeated resuscitation. He had impaired glucose tolerance. He died five weeks later and post-mortem examination revealed a myocardial abscess and sternal osteomyelitis due to Staphylococcus aureus. These had not been apparent clinically. The ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.65.766.589

    authors: Smith RE,Martin JE,Mills PG

    更新日期:1989-08-01 00:00:00

  • Occult pancreatic cancer with recurrent acute pancreatitis.

    abstract::A patient with occult pancreatic carcinoma associated with recurrent acute pancreatitis is reported. Endoscopic retrograde cholangiopancreatography after the first attack was normal, the diagnosis being made at repeat examination 10 months later. ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.62.730.765

    authors: Wilson C,Imrie CW

    更新日期:1986-08-01 00:00:00

  • Factors associated with high risk of marginal hyperthermia in elderly patients living in an institution.

    abstract::The elderly, the very young, and the sick are known to be adversely affected by high environmental temperatures. In a retrospective open case-note review of 872 patients in a large institution during a hot summer we identified characteristics in the elderly that increase the risk of marginal hyperthermia. Women were m...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.71.834.213

    authors: Vassallo M,Gera KN,Allen S

    更新日期:1995-04-01 00:00:00

  • Lack of response to octreotide in Cushing's syndrome due to metastatic adrenocortical carcinoma.

    abstract::Functional metastatic adrenocortical carcinoma is an uncommon cause of Cushing's syndrome, which rarely responds to conventional treatment. A patient presenting with Cushing's syndrome secondary to adrenocortical carcinoma underwent surgical resection. Postoperatively, she developed metastatic disease resistant to con...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.75.880.96

    authors: Chan NN,Isaacs AJ

    更新日期:1999-02-01 00:00:00

  • Intracoronary ultrasound.

    abstract::Although contrast angiography is important in the diagnosis and treatment of atherosclerotic disease, it does have limitations. Intracoronary ultrasound more accurately assesses the amount of atherosclerosis and has given us new insights into the pathophysiology of coronary plaque accumulation and remodelling. It also...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章,评审

    doi:10.1136/pgmj.74.874.468

    authors: Hammond C,Causer JP,Perry RA

    更新日期:1998-08-01 00:00:00

  • Knowledge and attitudes of breast self examination in a group of women in Shiraz, southern Iran.

    abstract:BACKGROUND:Breast cancer is the most common cause of cancer related deaths among women worldwide. The disease in women occurs at a younger age in Iran than in western communities. OBJECTIVE:To determine the practice of breast self examination (BSE) among 25-54-year-old women in Shiraz, southern Iran. METHODS:Using a ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.2008.072678

    authors: Simi A,Yadollahie M,Habibzadeh F

    更新日期:2009-06-01 00:00:00

  • Postgraduate training in family medicine in Albania.

    abstract::In the Balkan context education in general practice varies greatly and it is in different stages of reorganisation and curriculum development. The postgraduate training in family medicine in Albania started in January 1997 with the goal of providing future family doctors with the proper knowledge, skills, attitudes, a...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pmj.78.919.308

    authors: Rrumbullaku L,Theodorakis PN,Pulluqi P,Lionis C,Trell E

    更新日期:2002-05-01 00:00:00

  • Systemic inflammation: a key factor in the pathogenesis of cardiovascular complications in obstructive sleep apnoea syndrome?

    abstract::Obstructive sleep apnoea syndrome (OSAS) is a highly prevalent disease and is recognised as a major public health burden. Large-scale epidemiological studies have demonstrated an independent relationship between OSAS and various cardiovascular disorders. The pathogenesis of cardiovascular complications in OSAS is not ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/thx.2008.105577

    authors: Ryan S,Taylor CT,McNicholas WT

    更新日期:2009-12-01 00:00:00

  • Fungating carcinoma of the stomach: en bloc multiple organ resection and abdominal wall reconstruction.

    abstract::A patient with carcinoma of the stomach invading multiple adjacent organs and fungating through the anterior abdominal wall was treated by en bloc multiple organ resection and abdominal wall reconstruction. The patient is alive and well at the time of writing, six months after the operation. The rationale for embarkin...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.71.835.303

    authors: Chu KM,Lai DT,Stern HS,Sheldon DM

    更新日期:1995-05-01 00:00:00

  • Lobar collapse demystified: the chest radiograph with CT correlation.

    abstract::Collapse of a lobe of a lung is an important indicator of a range of conditions, including malignancy. Clinical symptoms and signs may suggest a diagnosis of lobar collapse; however, it is often diagnosed, and always needs to be confirmed, with radiological examination. The radiological signs may be subtle, difficult ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章,评审

    doi:10.1136/postgradmedj-2011-130213

    authors: Mullett R,Jain A,Kotugodella S,Curtis J

    更新日期:2012-06-01 00:00:00

  • Influence of nutrition in childhood on the origins of coronary heart disease.

    abstract::Both the quality and the quantity of food ingested are relevant to the genesis of risk factors for coronary heart disease and the two are inseparable. Nevertheless they have a major common pathway through hypertension, which may well be the most important consequence of a high-protein, high-carbohydrate, high-fat, hig...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.54.629.171

    authors: Brook CG

    更新日期:1978-03-01 00:00:00

  • Clinical distinction of cerebral haemorrhage and cerebral infarction.

    abstract::The clinical features of 62 cases of computerized axial tomography scan-proved cerebral haemorrhage and 81 cases of cerebral infarction in the carotid territory have been compared in a retrospective case note survey. The clinical features which can help in making the distinction between haemorrhage and infarction at t...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.56.659.629

    authors: Harrison MJ

    更新日期:1980-09-01 00:00:00

  • Therapy of Candida peritonitis: penetration of amphotericin B into peritoneal fluid.

    abstract::Candida albicans peritonitis developed in a 48-year-old man with a perforated gastric ulcer who subsequently was treated with intravenous amphotericin B. The drug penetrated well into the inflamed peritoneal cavity and eradicated the organism from the peritoneal fluid. Nevertheless, at post-mortem, Candida organisms w...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.54.631.340

    authors: Peterson LR,Kelty RH,Hall WH,Votava HJ

    更新日期:1978-05-01 00:00:00

  • Severe dothiepin intoxication--a report of two cases.

    abstract::Two patients with severe dothiepin intoxication are described. The management of these cases is discussed and we draw attention to the prolonged elimination half-life of dothiepin and its metabolites, and the implication for treatment. ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.60.704.442

    authors: Bloodworth L,Wilson A,Collins P,Rainford DJ

    更新日期:1984-06-01 00:00:00

  • Brittle Addison's disease: a new variation on a familiar theme.

    abstract::Unstable and unpredictable disease control in diabetes or asthma, with frequent hospitalisations, is frequently referred to as 'brittle'. We describe two cases of Addison's disease with recurrent hospitalisations in hypo-adrenal crises. Both patients had significant psychosocial disruption, and failure to take hydroco...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pmj.76.893.166

    authors: Gill GV,Williams G

    更新日期:2000-03-01 00:00:00

  • Strangulated femoral hernia: the persisting clinical trap.

    abstract::Five cases of strangulated femoral hernia, all with a delay in establishing the diagnosis, are reported here. These cases illustrate the continuing difficulties experienced, both in primary care and hospital practice, in making this diagnosis despite the clinical awareness of this condition. ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.67.783.57

    authors: Heys SD,Brittenden J

    更新日期:1991-01-01 00:00:00

  • Role of angiotensin-converting enzyme inhibitors in early antihypertensive treatment in non-insulin dependent diabetes mellitus.

    abstract::The effect of captopril monotherapy on blood pressure and metabolism was investigated in a placebo-controlled study in 30 non-insulin dependent (Type II) diabetic subjects during a 3-week observation period (run-in/drug; placebo/wash-out) on a metabolic ward. Captopril significantly reduced both systolic and diastolic...

    journal_title:Postgraduate medical journal

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Rett K,Wicklmayr M,Tschollar W,Dietze G,Mehnert H

    更新日期:1988-01-01 00:00:00

  • Elevated serum parathyroid hormone related protein and 1,25-dihydroxycholecalciferol in hypercalcaemia associated with adult T-cell leukaemia-lymphoma.

    abstract::Hypercalcaemia occurs in up to 80% of patients with adult T-cell leukaemia-lymphoma (ATLL) associated with human T-cell leukaemia virus-1 infection. Elevated serum levels of 1,25-dihydroxycholecalciferol, implicated in the pathogenesis of hypercalcaemia in lymphoma, and of parathyroid hormone-related protein (PTHrP), ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.68.803.753

    authors: Johnston SR,Hammond PJ

    更新日期:1992-09-01 00:00:00

  • Early-onset dementia: key issues using a relationship-centred care approach.

    abstract::People with early-onset dementia have a potential risk of being marginalised with respect to care and social support as a result of the blame and stigma associated with their condition, and because they have reduced access to treatment options and postdiagnostic care. The limited use of community services and the resu...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章,评审

    doi:10.1136/postgradmedj-2020-138517

    authors: Nwadiugwu M

    更新日期:2020-09-03 00:00:00

  • Subacute myelopathy caused by spinal venous infarction.

    abstract::A 44 year old female presented with a subacute myelopathy in association with pelvic venous thrombosis. It is inferred from the temporal relationship of these events that the patient suffered a subacute spinal venous infarction. This is discussed along with the aetiology, anatomical distribution and management of the ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.63.742.669

    authors: Clarke CE,Cumming WJ

    更新日期:1987-08-01 00:00:00

  • Localisation of the cricothyroid membrane by digital palpation in the emergency department.

    abstract:INTRODUCTION:A surgical approach to airway management may be essential in situations of difficult or failed airway, where immediate airway access is needed to provide oxygenation. However, the procedure is uncommonly performed and expertise among emergency clinicians may be limited. OBJECTIVES:The aim of this study wa...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/postgradmedj-2018-135828

    authors: Alshareef H,Al Saawi A,Almazroua F,Alyami H,Reilly GO,Mitra B

    更新日期:2018-08-01 00:00:00

  • Transient ischaemic attacks related to carotid stenosis precipitated by straining, bending, and sneezing.

    abstract::Three patients are described in whom one or more carotid territory transient ischaemic attacks (TIAs) were preceded by sneezing, straining, or bending over. It is argued that the mechanism involved dislodgment of embolic material from the site of carotid atheroma. This mechanism should be considered as an alternative ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.75.881.145

    authors: Harrison MJ

    更新日期:1999-03-01 00:00:00

  • Secretory symptoms from metastatic adrenal cortical carcinoma responding to octreotide.

    abstract::The prognosis of patients with metastatic adrenal cortical carcinoma is poor, and their disabling symptoms are usually unresponsive to conventional therapy. A patient with Cushing's syndrome secondary to a secretory adrenal cortical carcinoma was treated with octreotide, endocrine therapy and chemotherapy having faile...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.71.834.229

    authors: Pandha HS,Harrington K,Saini S,Lynn J,Peters M,Waxman J

    更新日期:1995-04-01 00:00:00

  • A case of multiple endocrine adenomatosis with primary amenorrhoea.

    abstract::A well documented sporadic case of multiple endocrine adenomatosis (MEA) type I, with the pituitary tumour presenting as a prolactinoma, is described in a 28-year-old female. Primary amenorrhoea, resulting from hyperprolactinaemia, was the first symptom of the polyglandular neoplasia. A gastrinoma was removed from the...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:10.1136/pgmj.54.635.618

    authors: Vandeweghe M,Braxel K,Schutyser J,Vermeulen A

    更新日期:1978-09-01 00:00:00

  • Agonist potencies of tryptamine derivatives at pre- and postjunctional receptors in canine saphenous vein.

    abstract::Strips form canine saphenous veins were incubated with (3H)-noradrenaline and mounted for superfusion. 5-Hydroxytryptamine (5-HT inhibited the release of tritium caused by sustained electrical stimulation (2 Hz, 150 mA, 0.1 msec) being about 10 times more potent than noradrenaline. 5-HT inhibited contractions induced ...

    journal_title:Postgraduate medical journal

    pub_type: 杂志文章

    doi:

    authors: Müller-Schweinitzer E

    更新日期:1981-01-01 00:00:00