Abstract：
:Mitochondrial dysfunction is associated with macrophage damage, but the role of mitochondrial fission in macrophage cholesterol metabolism is not fully understood. In this study, we explored the influences of miR-9 and mitochondrial fission on macrophage viability and cholesterol metabolism. Macrophages were incubated with oxidized low-density lipoprotein (ox-LDL) in vitro, after which mitochondrial fission, cell viability, and cholesterol metabolism were examined using qPCR, ELISAs, and immunofluorescence. ox-LDL treatment significantly increased Drp1-associated mitochondrial fission. Transfection of Drp1 siRNA significantly reduced cell death, attenuated oxidative stress, and inhibited inflammatory responses in ox-LDL-treated macrophages. Interestingly, inhibition of Drp1-related mitochondrial fission also improved cholesterol metabolism by balancing the transcription of cholesterol influx/efflux enzymes. We also found that miR-9 was downregulated in ox-LDL-treated macrophages, and administration of a miR-9 mimic decreased Drp1 transcription and mitochondrial fission, as well as its effects. These results indicate that signaling via the novel miR-9/Drp1/mitochondrial fission axis is a key determinant of macrophage viability and cholesterol metabolism.
journal_name
Oxid Med Cell Longevjournal_title
Oxidative medicine and cellular longevityauthors
Xin T,Lu C,Zhang J,Wen J,Yan S,Li C,Zhang F,Zhang Jdoi
10.1155/2020/8848930subject
Has Abstractpub_date
2020-11-01 00:00:00pages
8848930eissn
1942-0900issn
1942-0994journal_volume
2020pub_type
杂志文章abstract：:Neuroblastoma is a life-threatening extracranial solid tumor, preferentially occurring in children. However, its etiology remains unclear. APEX1 is a critical gene in the base excision repair (BER) system responsible for maintaining genome stability. Given the potential effects of APEX1 polymorphisms on the ability of...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/5736175
更新日期：2019-06-25 00:00:00
abstract：:Quercetin is a flavonoid widely studied as a chemopreventive agent in different types of cancer. Previously, we reported that quercetin has a chemopreventive effect on the liver-induced preneoplastic lesions in rats. Here, we evaluated if quercetin was able not only to prevent but also to reverse rat liver preneoplast...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2017/4674918
更新日期：2017-01-01 00:00:00
abstract：:The antiamnesic effect of 3,5-dicaffeoylquinic acid (3,5-diCQA) as the main phenolic compound in Artemisia argyi H. extract on cognitive dysfunction induced by trimethyltin (TMT) (7.1 μg/kg of body weight; intraperitoneal injection) was investigated in order to assess its ameliorating function in mice. In several beha...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/6981595
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:It has been known that cardiac surgery induces an oxidative stress. The persistent oxidative stress during reperfusion may lead to depressed myocardial function resulting in low cardiac output syndrome (LCOS) necessitating inotropic or intra-aortic balloon counterpulsation support. Total antioxidant capacity...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2012/356301
更新日期：2012-01-01 00:00:00
abstract：:We investigated the gender differences in heme-oxygenase (HO) enzyme, which produces endogenous vascular protective carbon monoxide (CO). We studied (1) the activity and expression of HO enzymes in the left ventricle (LV) and aorta, (2) basal increase in basal blood pressure provoked by arginine vasopressine (AVP) in ...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2013/521563
更新日期：2013-01-01 00:00:00
abstract：:Tilia genus is commonly used around the world for its central nervous system properties; it is prepared as tea and used as tranquilizing, anticonvulsant, and analgesic. In this study, anticonvulsant activity of the Tilia americana var. mexicana inflorescences and leaves was investigated by evaluating organic and aqueo...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2014/329172
更新日期：2014-01-01 00:00:00
abstract：:Fibroblasts are essential for tissue repair due to producing collagens, and lysosomal proteinase cathepsin B (CatB) is involved in promoting chronic inflammation. We herein report that CatB regulates the expression of collagens III and IV by fibroblasts in response to a TLR2 agonist, lipopolysaccharide from Porphyromo...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/7894247
更新日期：2016-01-01 00:00:00
abstract：:Hormone replacement therapy (HRT) can alleviate estrogen deficiency symptoms especially during menopause. The present study aimed at investigating the effect of soy isoflavones as HRT on immunological and bone health-related parameters with a special focus on the interactions between immunological status and metabolis...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/5713606
更新日期：2019-01-10 00:00:00
abstract：:As an essential lipid, cholesterol is of great value in keeping cell homeostasis, being the precursor of bile acid and steroid hormones, and stabilizing membrane lipid rafts. As a kind of cholesterol metabolite produced by enzymatic or radical process, oxysterols have drawn much attention in the last decades. Among wh...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2020/8893305
更新日期：2020-11-19 00:00:00
abstract：:Increasing evidence indicates that most of the tumors are sustained by a distinct population of cancer stem cells (CSCs), which are responsible for growth, metastasis, invasion, and recurrence. CSCs are typically characterized by self-renewal, the key biological process allowing continuous tumor proliferation, as well...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2015/425708
更新日期：2015-01-01 00:00:00
abstract：:Type 2 diabetes (T2DM) and its complications constitute a major worldwide public health problem, with high rates of morbidity and mortality. Biomarkers for predicting the occurrence and development of the disease may therefore offer benefits in terms of early diagnosis and intervention. This review provides an overvie...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2019/5953685
更新日期：2019-05-12 00:00:00
abstract：:Doxorubicin (DOX) is a broad-spectrum antitumor drug while its use is limited due to its neurobiological side effects associated with depression. We investigated the neuroprotective efficacy of dl-3-n-butylphthalide (dl-NBP) against DOX-induced anxiety- and depression-like behaviors in rats. dl-NBP was given (30 mg/kg...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/9125601
更新日期：2018-08-16 00:00:00
abstract：Background:Infrasound is a major threat to global health by causing injuries of the central nervous system (CNS). However, there remains no effective therapeutic agent for preventing infrasound-caused CNS injury. 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glycoside (THSG) exerts protective function against CNS injuries and ...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2020/6576718
更新日期：2020-01-06 00:00:00
abstract：:Platelet-rich plasma (PRP) has grown as an attractive biologic instrument in regenerative medicine for its powerful healing properties. It is considered as a source of growth factors that may induce tissue repairing and improve fibrosis. This product has proven its efficacy in multiple studies, but its effect on cispl...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/9658230
更新日期：2018-07-18 00:00:00
abstract：:Essential oils of some aromatic plants provide an effective nonmedicinal option to control liver fibrosis. Mentha piperita L. essential oil (MPEO) have been reported to possess protective effects against hepatotoxicity. However, its effect against liver fibrosis remains unknown. The present study investigated the anti...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/4039753
更新日期：2018-04-19 00:00:00
abstract：:Patients with triple negative breast cancer (TNBC) often suffer relapse, and clinical improvements offered by radiotherapy and chemotherapy are modest. Although targeted therapy and immunotherapy have been a topic of significant research in recent years, scientific developments have not yet translated to significant i...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2020/7152173
更新日期：2020-11-05 00:00:00
abstract：:Background. Increased oxidative stress is a well described feature of patients in hemodialysis. Their need for multiple blood transfusions and supplemental iron causes a significant iron overload that has recently been associated with increased oxidation of polyunsaturated lipids and accelerated aging due to DNA damag...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/1578235
更新日期：2016-01-01 00:00:00
abstract：:Aconitase, the second enzyme of the tricarboxylic acid cycle encoded by ACO1 in the budding yeast Saccharomyces cerevisiae, catalyzes the conversion of citrate to isocitrate. aco1Δ results in mitochondrial DNA (mtDNA) instability. It has been proposed that Aco1 binds to mtDNA and mediates its maintenance. Here we prop...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2013/493536
更新日期：2013-01-01 00:00:00
abstract：:Inflammation and oxidative stress are pivotal mechanisms for the pathogenesis of ischemia and reperfusion injury (IRI). Vagus nerve stimulation (VNS) may participate in maintaining oxidative homeostasis and response to external stimulus or injury. We investigated whether the in vivo VNS can protect the liver from IRI....
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2020/1079129
更新日期：2020-01-21 00:00:00
abstract：:This study is aimed at investigating the cytotoxicity, anti-inflammatory, and angiogenic activities of two Boswellia serrata extracts on primary culture of porcine aortic endothelial cells (pAECs). Chemical characterization of a dry extract (extract A) and a hydroenzymatic extract (extract G) of B. serrata was perform...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/2504305
更新日期：2018-06-25 00:00:00
abstract：:Detection of free radicals in tissues is challenging. Most approaches rely on incubating excised sections or homogenates with reagents, typically at supraphysiologic oxygen tensions, to finally detect surrogate, nonspecific end products. In the present work, we explored the potential of using intravenously (i.v.) inje...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/7838406
更新日期：2019-05-22 00:00:00
abstract：:Hydrogen sulfide (H2S) is a gasotransmitter with a variety of cardiovascular protective effects. Sirtuin 3 (SIRT3) is closely related to mitochondrial function and oxidative stress. We found that NaHS increased SIRT3 expression in the preventive effect on isoproterenol- (ISO-) induced myocardial hypertrophy. We furthe...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/9396089
更新日期：2018-12-17 00:00:00
abstract：:The purpose of this study was to investigate the modulation of histone deacetylase 2 (HDAC2) on mitochondrial apoptosis in acute liver failure (ALF). The cellular model was established with LO2 cells stimulated by tumor necrosis factor alpha (TNF-α)/D-galactosamine (D-gal). Rats were administrated by lipopolysaccharid...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/8173016
更新日期：2019-04-28 00:00:00
abstract：:Galectin-3 is an important modulator of several biological functions. It has been implicated in numerous disease conditions, particularly in the long-term complications of diabetes because of its ability to bind the advanced glycation/lipoxidation end products that accumulate in target organs and exert their toxic eff...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2016/9618092
更新日期：2016-01-01 00:00:00
abstract：:Recent studies have found that propofol may protect brain from cerebral ischemic-reperfusion injury. However, the underlying mechanism remains unclear. The effects of propofol were evaluated in HBVSMC after hypoxia/reoxygenation (H/R). Cell viability and levels of SOD, LDH, and MDA were measured. Apoptosis was detecte...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/1518738
更新日期：2016-01-01 00:00:00
abstract：:Undiminishing actuality of enzyme modification for therapeutic purposes has been confirmed by application of modified enzymes in clinical practice and numerous research data on them. Intravenous injection of the superoxide dismutase-chondroitin sulfate-catalase (SOD-CHS-CAT) conjugate in preventive and medicative regi...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2016/3075695
更新日期：2016-01-01 00:00:00
abstract：:Allicin was discussed as an active compound with regard to the beneficial effects of garlic in atherosclerosis. The aim of this study was to investigate the cholesterol-lowering properties of allicin. In order to examine its effects on hypercholesterolemia in male ICR mice, this compound with doses of 5, 10, or 20 mg/...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2012/489690
更新日期：2012-01-01 00:00:00
abstract：:Mechanisms underlying hypertensive target organ damage (TOD) are not completely understood. The pathophysiological role of mitochondrial oxidative stress, resulting from mitochondrial dysfunction, in development of TOD is unclear. The stroke-prone spontaneously hypertensive rat (SHRSP) is a suitable model of human hyp...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2016/1067801
更新日期：2016-01-01 00:00:00
abstract：:Methamphetamine (MA) leads to cardiac and pulmonary toxicity expressed as increases in inflammatory responses and oxidative stress. However, some interactions may exist between oxidative stress and endoplasmic reticulum stress (ERS). The current study is designed to investigate if both oxidative stress and ERS are inv...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2017/4310475
更新日期：2017-01-01 00:00:00
abstract：:Hyperthyroidism is an endocrine disorder characterized by excessive secretion of thyroid hormones T3 and T4. Thyroid hormones exert pleiotropic actions on numerous tissues and induce an overall increase in metabolism, with an increase in energy demand and oxygen consumption. Therefore, the purpose of this study was to...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/7471890
更新日期：2019-06-02 00:00:00
