Rice FLOURY ENDOSPERM 18 encodes a pentatricopeptide repeat protein required for 5' processing of mitochondrial nad5 mRNA and endosperm development.

Abstract:

:Pentatricopeptide repeat (PPR) proteins, composing one of the largest protein families in plants, are involved in RNA binding and regulation of organelle RNA metabolism at the post-transcriptional level. Although several PPR proteins have been implicated in endosperm development in rice (Oryza sativa), the molecular functions of many PPRs remain obscure. Here, we identified a rice endosperm mutant named floury endosperm 18 (flo18) with pleiotropic defects in both reproductive and vegetative development. Map-based cloning and complementation tests showed that FLO18 encodes a mitochondrion-targeted P-type PPR protein with 15 PPR motifs. Mitochondrial function was disrupted in the flo18 mutant, as evidenced by decreased assembly of Complex I in the mitochondrial electron transport chain and altered mitochondrial morphology. Loss of FLO18 function resulted in defective 5'-end processing of mitochondrial nad5 transcripts encoding subunit 5 of NADH dehydrogenase. These results suggested that FLO18 is involved in 5'-end processing of nad5 mRNA and plays an important role in mitochondrial function and endosperm development. This article is protected by copyright. All rights reserved.

journal_name

J Integr Plant Biol

authors

Yu M,Wu M,Ren Y,Wang Y,Li J,Lei C,Sun Y,Bao X,Wu H,Yang H,Pan T,Wang Y,Jing R,Yan M,Zhang H,Zhao L,Zhao Z,Zhang X,Guo X,Cheng Z,Yang B,Jiang L,Wan J

doi

10.1111/jipb.13049

subject

Has Abstract

pub_date

2020-12-07 00:00:00

eissn

1672-9072

issn

1744-7909

pub_type

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