Genome editing for plant research and crop improvement.


:The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state-of-the-art development of CRISPR technologies and their applications in plants, from the initial introduction of random small indel (insertion or deletion) mutations at target genomic loci to precision editing such as base editing, prime editing and gene targeting. We describe advances in the use of class 2, types II, V, and VI systems for gene disruption as well as for precise sequence alterations, gene transcription, and epigenome control.


J Integr Plant Biol


Zhan X,Lu Y,Zhu JK,Botella JR




Has Abstract


2021-01-01 00:00:00












  • Strategic conservation of orchard germplasm based on indigenous knowledge and genetic diversity: a case study of sour orange populations in China.

    abstract::To effectively conserve sour orange (Citrus aurantium L.) germplasm on two islands at the estuary of the Yangtze River in China, we estimated genetic variation and relationships of the known parental trees and their proposed descendents (young trees) using the fingerprints of random amplified polymorphic DNA (RAPD). R...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Ming F,Liu QK,Shi JL,Wang W,Lu BR

    更新日期:2009-01-01 00:00:00

  • Physiological traits contributed to the recent increase in yield potential of winter wheat from Henan Province, China.

    abstract::This experiment aims to test the traits responsible for the increase in yield potential of winter wheat released in Henan Province, China. Seven established cultivars released in the last 20 years and three advanced lines were assayed. The results showed that grain yield was positively correlated with harvest index (H...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Zhou B,Sanz-Sáez Á,Elazab A,Shen T,Sánchez-Bragado R,Bort J,Serret MD,Araus JL

    更新日期:2014-05-01 00:00:00

  • Molecular processes in iron and zinc homeostasis and their modulation for biofortification in rice.

    abstract::More than a billion people suffer from iron or zinc deficiencies globally. Rice (Oryza sativa L.) iron and zinc biofortification; i.e., intrinsic iron and zinc enrichment of rice grains, is considered the most effective way to tackle these deficiencies. However, rice iron biofortification, by means of conventional bre...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章,评审


    authors: Kawakami Y,Bhullar NK

    更新日期:2018-12-01 00:00:00

  • Two-stage hydrolysis of invasive algal feedstock for ethanol fermentation.

    abstract::The overall goal of this work was to develop a saccharification method for the production of third generation biofuel (i.e. bioethanol) using feedstock of the invasive marine macroalga Gracilaria salicornia. Under optimum conditions (120 °C and 2% sulfuric acid for 30 min), dilute acid hydrolysis of the homogenized in...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Wang X,Liu X,Wang G

    更新日期:2011-03-01 00:00:00

  • Plant plasma membrane-resident receptors: Surveillance for infections and coordination for growth and development.

    abstract::As sessile organisms, plants are exposed to pathogen invasions and environmental fluctuations. To overcome the challenges of their surroundings, plants acquire the potential to sense endogenous and exogenous cues, resulting in their adaptability. Hence, plants have evolved a large collection of plasma membrane-residen...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章,评审


    authors: Escocard de Azevedo Manhães AM,Ortiz-Morea FA,He P,Shan L

    更新日期:2021-01-01 00:00:00

  • Molecular mechanisms governing plant responses to high temperatures.

    abstract::The increased prevalence of high temperatures (HTs) around the world is a major global concern, as they dramatically affect agronomic productivity. Upon HT exposure, plants sense the temperature change and initiate cellular and metabolic responses that enable them to adapt to their new environmental conditions. Decodi...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章,评审


    authors: Li B,Gao K,Ren H,Tang W

    更新日期:2018-09-01 00:00:00

  • The C-terminal 17 amino acids of the photoreceptor UVR8 is involved in the fine-tuning of UV-B signaling.

    abstract::Plant UV-B responses are mediated by the photoreceptor UV RESISTANCE LOCUS 8 (UVR8). In response to UV-B irradiation, UVR8 homodimers dissociate into monomers that bind to the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). The interaction of the C27 domain in the C-terminal tail of UVR8 with the WD40 domai...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Lin L,Dong H,Yang G,Yin R

    更新日期:2020-09-01 00:00:00

  • Arabidopsis acetyl-amido synthetase GH3.5 involvement in camalexin biosynthesis through conjugation of indole-3-carboxylic acid and cysteine and upregulation of camalexin biosynthesis genes.

    abstract::Camalexin (3-thiazol-2'-yl-indole) is the major phytoalexin found in Arabidopsis thaliana. Several key intermediates and corresponding enzymes have been identified in camalexin biosynthesis through mutant screening and biochemical experiments. Camalexin is formed when indole-3-acetonitrile (IAN) is catalyzed by the cy...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Wang MY,Liu XT,Chen Y,Xu XJ,Yu B,Zhang SQ,Li Q,He ZH

    更新日期:2012-07-01 00:00:00

  • Sequence information on simple sequence repeats and single nucleotide polymorphisms through transcriptome analysis of mungbean.

    abstract::Mungbean (Vigna radiata (L.) Wilczek) is a unique species in its ability to fix atmospheric nitrogen, with early maturity, and relatively good drought resistance. We used 454 sequencing technology for transcriptome sequencing. A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs (≥ 500 bp) with ...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Moe KT,Chung JW,Cho YI,Moon JK,Ku JH,Jung JK,Lee J,Park YJ

    更新日期:2011-01-01 00:00:00

  • A zinc finger protein, interacted with cyclophilin, affects root development via IAA pathway in rice.

    abstract::The plant hormone auxin plays a crucial role in lateral root development. To better understand the molecular mechanisms underlying lateral root formation, an auxin-responsive gene OsCYP2 (Os02g0121300) was characterized from rice. Compared to the wild type, OsCYP2-RNAi (RNA interference) lines exhibited distinctive de...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Cui P,Liu H,Ruan S,Ali B,Gill RA,Ma H,Zheng Z,Zhou W

    更新日期:2017-07-01 00:00:00

  • Phototropin- and photosynthesis-dependent mitochondrial positioning in Arabidopsis thaliana mesophyll cells.

    abstract::Mitochondria are frequently observed in the vicinity of chloroplasts in photosynthesizing cells, and this association is considered necessary for their metabolic interactions. We previously reported that, in leaf palisade cells of Arabidopsis thaliana, mitochondria exhibit blue-light-dependent redistribution together ...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Islam MS,Van Nguyen T,Sakamoto W,Takagi S

    更新日期:2020-09-01 00:00:00

  • Overexpression of microRNA408 enhances photosynthesis, growth, and seed yield in diverse plants.

    abstract::The ability of a plant to produce grain, fruit, or forage depends ultimately on photosynthesis. There have been few attempts, however, to study microRNAs, which are a class of endogenous small RNAs post-transcriptionally programming gene expression, in relation to photosynthetic traits. We focused on miR408, one of th...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Pan J,Huang D,Guo Z,Kuang Z,Zhang H,Xie X,Ma Z,Gao S,Lerdau MT,Chu C,Li L

    更新日期:2018-04-01 00:00:00

  • Characterization of two putative protein phosphatase genes and their involvement in phosphorus efficiency in Phaseolus vulgaris.

    abstract::Protein dephosphorylation mediated by protein phosphatases plays a major role in signal transduction of plant responses to environmental stresses. In this study, two putative protein phosphatases, PvPS2:1 and PvPS2:2 were identified and characterized in bean (Phaseolus vulgaris). The two PvPS2 members were found to be...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Liang CY,Chen ZJ,Yao ZF,Tian J,Liao H

    更新日期:2012-06-01 00:00:00

  • Evolution and protein interactions of AP2 proteins in Brassicaceae: Evidence linking development and environmental responses.

    abstract::Plants have evolved a large number of transcription factors (TF), which are enriched among duplicate genes, highlighting their roles in complex regulatory networks. The APETALA2/EREBP-like genes constitute a large plant TF family and participate in development and stress responses. To probe the conservation and diverg...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Zeng L,Yin Y,You C,Pan Q,Xu D,Jin T,Zhang B,Ma H

    更新日期:2016-06-01 00:00:00

  • Crossability barriers in the interspecific hybridization between Oryza sativa and O. meyeriana.

    abstract::Oryza meyeriana Baill (GG genome) is a precious germplasm in the tertiary gene pool of cultivated rice (AA genome), and possesses important traits such as resistance and tolerance to biotic and abiotic stress. However, interspecific crossability barrier, a critical bottleneck restricting genes transfer from O. meyeria...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Fu XL,Lu YG,Liu XD,Li JQ

    更新日期:2009-01-01 00:00:00

  • Relationship between the virtual dynamic thinning line and the self-thinning boundary line in simulated plant populations.

    abstract::The self-thinning rule defines a straight upper boundary line on log-log scales for all possible combinations of mean individual biomass and density in plant populations. Recently, the traditional slope of the upper boundary line, -3/2, has been challenged by -4/3 which is deduced from some new mechanical theories, li...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Chen K,Kang HM,Bai J,Fang XW,Wang G

    更新日期:2008-03-01 00:00:00

  • Hydrogen sulfide, a signaling molecule in plant stress responses.

    abstract::Gaseous molecules, such as hydrogen sulfide (H2 S) and nitric oxide (NO), are crucial players in cellular and (patho)physiological processes in biological systems. The biological functions of these gaseous molecules, which were first discovered and identified as gasotransmitters in animals, have received unprecedented...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章,评审


    authors: Zhang J,Zhou M,Zhou H,Zhao D,Gotor C,Romero LC,Shen J,Ge Z,Zhang Z,Shen W,Yuan X,Xie Y

    更新日期:2021-01-01 00:00:00

  • A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency.

    abstract::In rice (Oryza sativa), amylose content (AC) is the major factor that determines eating and cooking quality (ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy (Wx) locus. Here we identified a rare Wx allele, Wxmw , which combines a favorable AC, improved ECQ and grain transparenc...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Zhang C,Yang Y,Chen S,Liu X,Zhu J,Zhou L,Lu Y,Li Q,Fan X,Tang S,Gu M,Liu Q

    更新日期:2020-09-04 00:00:00

  • Arabidopsis chloroplast FtsH, var2 and suppressors of var2 leaf variegation: a review.

    abstract::Variegation mutants are ideal model systems to study chloroplast biogenesis. We are interested in variegations whose green and white-sectored leaves arise as a consequence of the action of nuclear recessive genes. In this review, we focus on the Arabidopsis var2 variegation mutant, and discuss recent progress toward u...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章,评审


    authors: Liu X,Yu F,Rodermel S

    更新日期:2010-08-01 00:00:00

  • Integrated metabolo-transcriptomics and functional characterization reveals that the wheat auxin receptor TIR1 negatively regulates defense against Fusarium graminearum.

    abstract::Fusarium head blight (FHB) caused by Fusarium graminearum Schwabe (teleomorph Gibberella zeae (Schw.) Perch) results in large yield losses in annual global wheat production. Although studies have identified a number of wheat FHB resistance genes, a deeper understanding of the mechanisms underlying host plant resistanc...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Su P,Zhao L,Li W,Zhao J,Yan J,Ma X,Li A,Wang H,Kong L

    更新日期:2020-07-17 00:00:00

  • Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution.

    abstract::We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 (PGR5) capable of producing hydrogen (H2 ) for 25 days with more than 30-fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H2 production than a previously characterized pgr5 mutant. Physiological and...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Chen M,Zhang J,Zhao L,Xing J,Peng L,Kuang T,Rochaix JD,Huang F

    更新日期:2016-12-01 00:00:00

  • The origin of Wxla provides new insights into the improvement of grain quality in rice.

    abstract::Appearance and taste are important factors in rice (Oryza sativa) grain quality. Here, we investigated the taste scores and related eating-quality traits of 533 diverse cultivars to assess the relationships between-and genetic basis of-rice taste and eating-quality. A genome-wide association study highlighted the Wx g...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Zhou H,Xia D,Zhao D,Li Y,Li P,Wu B,Gao G,Zhang Q,Wang G,Xiao J,Li X,Yu S,Lian X,He Y

    更新日期:2020-09-04 00:00:00

  • Silencing NOA1 elevates herbivory-induced jasmonic acid accumulation and compromises most of the carbon-based defense metabolites in Nicotiana attenuata(F).

    abstract::Nitric oxide-associated protein 1 (NOA1) is involved in various abiotic stress responses and is required for plant resistance to pathogen infections. However, the role of NOA1 in plant-herbivore interactions has not been explored. We created NOA1-silenced Nicotiana attenuata plants (irNaNOA1). Compared with wild-type ...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Wünsche H,Baldwin IT,Wu J

    更新日期:2011-08-01 00:00:00

  • The endoplasmic reticulum: a dynamic and well-connected organelle.

    abstract::The endoplasmic reticulum forms the first compartment in a series of organelles which comprise the secretory pathway. It takes the form of an extremely dynamic and pleomorphic membrane-bounded network of tubules and cisternae which have numerous different cellular functions. In this review, we discuss the nature of en...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章,评审


    authors: Hawes C,Kiviniemi P,Kriechbaumer V

    更新日期:2015-01-01 00:00:00

  • Nia1 and Nia2 are involved in exogenous salicylic acid-induced nitric oxide generation and stomatal closure in Arabidopsis.

    abstract::Phytohormone salicylic acid (SA) plays important roles in plant responses to environmental stress. However, knowledge about the molecular mechanisms for SA affecting the stomatal movements is limited. In this paper, we demonstrated that exogenous SA significantly induced stomatal closure and nitric oxide (NO) generati...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Hao F,Zhao S,Dong H,Zhang H,Sun L,Miao C

    更新日期:2010-03-01 00:00:00

  • From filaments to function: The role of the plant actin cytoskeleton in pathogen perception, signaling and immunity.

    abstract::The eukaryotic actin cytoskeleton is required for numerous cellular processes, including cell shape, development and movement, gene expression and signal transduction, and response to biotic and abiotic stress. In recent years, research in both plants and animal systems have described a function for actin as the ideal...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章,评审


    authors: Porter K,Day B

    更新日期:2016-04-01 00:00:00

  • A novel mitogen-activated protein kinase gene in maize (Zea mays), ZmMPK3, is involved in response to diverse environmental cues.

    abstract::In search for components of mitogen-activated protein kinase (MAPK) cascades in maize (Zea mays) involved in response to abscisic acid (ABA) stimulus, a novel MAPK gene, ZmMPK3, from ABA-treated maize leaves cDNA was isolated and characterized. The full length of the ZmMPK3 gene is 1 520 bp and encodes a 376 amino aci...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Wang J,Ding H,Zhang A,Ma F,Cao J,Jiang M

    更新日期:2010-05-01 00:00:00

  • Metabolism and long-distance translocation of cytokinins.

    abstract::During plant development, distantly-located organs must communicate in order to adapt morphological and physiological features in response to environmental inputs. Among the recognized signaling molecules, a class of phytohormones known as the cytokinins functions as both local and long-distance regulatory signals for...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章,评审


    authors: Kudo T,Kiba T,Sakakibara H

    更新日期:2010-01-01 00:00:00

  • Rice FLOURY ENDOSPERM 18 encodes a pentatricopeptide repeat protein required for 5' processing of mitochondrial nad5 mRNA and endosperm development.

    abstract::Pentatricopeptide repeat (PPR) proteins, composing one of the largest protein families in plants, are involved in RNA binding and regulation of organelle RNA metabolism at the post-transcriptional level. Although several PPR proteins have been implicated in endosperm development in rice (Oryza sativa), the molecular f...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Yu M,Wu M,Ren Y,Wang Y,Li J,Lei C,Sun Y,Bao X,Wu H,Yang H,Pan T,Wang Y,Jing R,Yan M,Zhang H,Zhao L,Zhao Z,Zhang X,Guo X,Cheng Z,Yang B,Jiang L,Wan J

    更新日期:2020-12-07 00:00:00

  • Circadian clock component, LHY, tells a plant when to respond photosynthetically to light in nature.

    abstract::The circadian clock is known to increase plant growth and fitness, and is thought to prepare plants for photosynthesis at dawn and dusk; whether this happens in nature was unknown. We transformed the native tobacco, Nicotiana attenuata to silence two core clock components, NaLHY (irLHY) and NaTOC1 (irTOC1). We charact...

    journal_title:Journal of integrative plant biology

    pub_type: 杂志文章


    authors: Joo Y,Fragoso V,Yon F,Baldwin IT,Kim SG

    更新日期:2017-08-01 00:00:00