Abstract:
:The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state-of-the-art development of CRISPR technologies and their applications in plants, from the initial introduction of random small indel (insertion or deletion) mutations at target genomic loci to precision editing such as base editing, prime editing and gene targeting. We describe advances in the use of class 2, types II, V, and VI systems for gene disruption as well as for precise sequence alterations, gene transcription, and epigenome control.
journal_name
J Integr Plant Bioljournal_title
Journal of integrative plant biologyauthors
Zhan X,Lu Y,Zhu JK,Botella JRdoi
10.1111/jipb.13063subject
Has Abstractpub_date
2021-01-01 00:00:00pages
3-33issue
1eissn
1672-9072issn
1744-7909journal_volume
63pub_type
杂志文章,评审abstract::Auxin is a crucial phytohormone, controlling multiple aspects of plant growth and responses to the changing environment. However, the role of local auxin biosynthesis in specific developmental programs remains unknown in crops. This study characterized the rice tillering and small grain 1 (tsg1) mutant, which has more...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12820
更新日期:2020-05-01 00:00:00
abstract::Camalexin (3-thiazol-2'-yl-indole) is the major phytoalexin found in Arabidopsis thaliana. Several key intermediates and corresponding enzymes have been identified in camalexin biosynthesis through mutant screening and biochemical experiments. Camalexin is formed when indole-3-acetonitrile (IAN) is catalyzed by the cy...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2012.01131.x
更新日期:2012-07-01 00:00:00
abstract::Maize seedlings are very sensitive to chilling, especially during the transition phase from heterotrophic to autotrophic growth. Genetic dissection of the genetic basis of chilling tolerance would provide useful information for genetic improvement of maize inbreds. In this study, genome-wide association analysis was c...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12051
更新日期:2013-08-01 00:00:00
abstract::Polyamines play an important role in plant response to abiotic stress. S-adenosyl-l-methionine decarboxylase (SAMDC) is one of the key regulatory enzymes in the biosynthesis of polyamines. In order to better understand the effect of regulation of polyamine biosynthesis on the tolerance of high-temperature stress in to...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2009.00816.x
更新日期:2009-05-01 00:00:00
abstract::Pollen development is a critical step in plant development that is needed for successful breeding and seed formation. Manipulation of male fertility has proved a useful trait for hybrid breeding and increased crop yield. However, although there is a good understanding developing of the molecular mechanisms of anther a...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/jipb.12425
更新日期:2015-11-01 00:00:00
abstract::A striking characteristic of plant cells is that their organelles can move rapidly through the cell. This movement, commonly referred to as cytoplasmic streaming, has been observed for over 200 years, but we are only now beginning to decipher the mechanisms responsible for it. The identification of the myosin motor pr...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/j.1744-7909.2012.01180.x
更新日期:2012-11-01 00:00:00
abstract::The interaction between plants and pathogens represents a dynamic competition between a robust immune system and efficient infectious strategies. Plant innate immunity is composed of complex and highly regulated molecular networks, which can be triggered by the perception of either conserved or race-specific pathogeni...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/jipb.12200
更新日期:2014-10-01 00:00:00
abstract::Gene expression studies are important for revealing gene functions putatively involved in biological processes. We were interested in identifying differentially expressed genes during leaf development in rice. We combined the RNA arbitrarily primed-polymerase chain reaction (RAP-PCR) and dot blot hybridization methods...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2008.00801.x
更新日期:2009-06-01 00:00:00
abstract::The circadian clock is known to increase plant growth and fitness, and is thought to prepare plants for photosynthesis at dawn and dusk; whether this happens in nature was unknown. We transformed the native tobacco, Nicotiana attenuata to silence two core clock components, NaLHY (irLHY) and NaTOC1 (irTOC1). We charact...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12547
更新日期:2017-08-01 00:00:00
abstract::The thermosensitive genic male sterile 5 (tms5) mutation causes thermosensitive genic male sterility in rice (Oryza sativa) through loss of RNase ZS1 function, which influences ubiquitin fusion ribosomal protein L40 (UbL40 ) messenger RNA levels during male development. Here, we used ATAC-seq, combined with analysis o...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12871
更新日期:2020-07-01 00:00:00
abstract::Although pollen tube growth is a prerequisite for higher plant fertilization and seed production, the processes leading to pollen tube emission and elongation are crucial for understanding the basic mechanisms of tip growth. It was generally accepted that pollen tube elongation occurs by accumulation and fusion of Gol...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/j.1744-7909.2009.00842.x
更新日期:2009-08-01 00:00:00
abstract::The main objective of the present study was to identify simple sequence repeat (SSR) markers associated with the amino acid content of rice (Oryza sativa L.). SSR markers were selected by prescreening for the relationship to amino acid content. Eighty-four rice landrace accessions from Korea were evaluated for 16 kind...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2009.00883.x
更新日期:2009-12-01 00:00:00
abstract::A narrow leaf mutant was isolated from transgenic rice (Oryza sativa L.) lines carrying a T-DNA insertion. The mutant is characterized by narrow leaves during its whole growth period, and was named nal9 (narrow leaf 9). The mutant also has other phenotypes, such as light green leaves at the seedling stage, reduced pla...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12098
更新日期:2013-11-01 00:00:00
abstract::Uniformity of stem height in rice directly affects crop yield potential and appearance, and has become a vital index for rice improvement. In the present study, a doubled haploid (DH) population, derived from a cross between japonica rice Chunjiang 06 and indica rice TN1 was used to analyze the quantitative trait locu...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2009.00854.x
更新日期:2009-09-01 00:00:00
abstract::Because Iron (Fe) is an essential element, Fe storage in plant seeds is necessary for seedling establishment following germination. However, the mechanisms controlling seed Fe storage during seed development remain largely unknown. Here we reveal that an ERF95 transcription factor regulates Arabidopsis seed Fe accumul...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12986
更新日期:2020-08-01 00:00:00
abstract::Gene expression in chloroplasts is regulated by many nuclear-encoded proteins. In this study, we isolated a rice (Oryza sativa subsp. japonica) mutant osotp51 with significant reduction in photosystem I (PSI). The osotp51 is extremely sensitive to light and accumulates a higher level of reactive oxygen species. Its le...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2012.01094.x
更新日期:2012-02-01 00:00:00
abstract::Precise replacement of an existing allele in commercial cultivars with an elite allele is a major goal in crop breeding. A single nucleotide polymorphism in the NRT1.1B gene between japonica and indica rice is responsible for the improved nitrogen use efficiency in indica rice. Herein, we precisely replaced the japoni...
journal_title:Journal of integrative plant biology
pub_type: 信件
doi:10.1111/jipb.12650
更新日期:2018-07-01 00:00:00
abstract::Fusarium head blight (FHB) caused by Fusarium graminearum Schwabe (teleomorph Gibberella zeae (Schw.) Perch) results in large yield losses in annual global wheat production. Although studies have identified a number of wheat FHB resistance genes, a deeper understanding of the mechanisms underlying host plant resistanc...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12992
更新日期:2020-07-17 00:00:00
abstract::The juvenile-to-adult transition in plants involves changes in vegetative growth and plant architecture; the timing of this transition has important implications for agriculture. The microRNA miR156 regulates this transition and shoot maturation in plants. In Arabidopsis thaliana, deposition of histone H3 trimethylati...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12991
更新日期:2020-12-01 00:00:00
abstract::Heat stress is a major abiotic stress limiting plant growth and productivity in many areas of the world. Understanding mechanisms of plant adaptation to heat stress would facilitate the development of heat-tolerant cultivars for improving productivity in warm climatic regions. Protein metabolism involving protein synt...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/j.1744-7909.2008.00735.x
更新日期:2008-10-01 00:00:00
abstract::To effectively conserve sour orange (Citrus aurantium L.) germplasm on two islands at the estuary of the Yangtze River in China, we estimated genetic variation and relationships of the known parental trees and their proposed descendents (young trees) using the fingerprints of random amplified polymorphic DNA (RAPD). R...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2008.00768.x
更新日期:2009-01-01 00:00:00
abstract::During daylight, plants produce excess photosynthates, including sucrose, which is temporarily stored in the vacuole. At night, plants remobilize sucrose to sustain metabolism and growth. Based on homology to other sucrose transporter (SUT) proteins, we hypothesized the maize (Zea mays) SUCROSE TRANSPORTER2 (ZmSUT2) p...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12527
更新日期:2017-06-01 00:00:00
abstract::Jasmonates (JAs) are rapidly induced after wounding and act as key regulators for wound induced signaling pathway. However, what perceives the wound signal and how that triggers JA biosynthesis remains poorly understood. To identify components involved in Arabidopsis wound and JA signaling pathway, we screened for mut...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12427
更新日期:2016-06-01 00:00:00
abstract::We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co-expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and ef...
journal_title:Journal of integrative plant biology
pub_type: 信件
doi:10.1111/jipb.12667
更新日期:2018-08-01 00:00:00
abstract::Nitric oxide-associated protein 1 (NOA1) is involved in various abiotic stress responses and is required for plant resistance to pathogen infections. However, the role of NOA1 in plant-herbivore interactions has not been explored. We created NOA1-silenced Nicotiana attenuata plants (irNaNOA1). Compared with wild-type ...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2011.01040.x
更新日期:2011-08-01 00:00:00
abstract::Hydrogen sulfide (H2 S) is a newly-discovered signaling molecule in plants and has caused increasing attention in recent years, but its function in stomatal movement is unclear. In plants, H2 S is synthesized via cysteine degradation catalyzed by D-/L-cysteine desulfhydrase (D-/L-CDes). AtD-/L-CDes::GUS transgenic Ara...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12004
更新日期:2013-03-01 00:00:00
abstract::Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate (EMS)-induced soybean (Glycine max) population, consisting of 21,600 independent M2 lines, was developed. Over 1,000 M4 (5) families, with diverse abnorm...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12505
更新日期:2017-01-01 00:00:00
abstract::Photosynthesis in "green" seeds, such as rapeseed, soybean, and Arabidopsis, plays a substantial role in the improved efficiency of oil accumulation. However, the molecular mechanism underpinning the coordinated expression of fatty acid (FA) biosynthesis- and photosynthesis-related genes in such developing seeds remai...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12158
更新日期:2014-06-01 00:00:00
abstract::Oryza meyeriana Baill (GG genome) is a precious germplasm in the tertiary gene pool of cultivated rice (AA genome), and possesses important traits such as resistance and tolerance to biotic and abiotic stress. However, interspecific crossability barrier, a critical bottleneck restricting genes transfer from O. meyeria...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2008.00728.x
更新日期:2009-01-01 00:00:00
abstract::It was noted that circadian components function in plant adaptation to diurnal temperature cycles and freezing tolerance. Our genome-wide transcriptome analysis revealed that evening-phased COR27 and COR28 mainly repress the transcription of clock-associated evening genes PRR5, ELF4 and cold-responsive genes. Chromati...
journal_title:Journal of integrative plant biology
pub_type: 信件
doi:10.1111/jipb.12512
更新日期:2017-02-01 00:00:00