Genome editing for plant research and crop improvement.

Abstract:

:The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state-of-the-art development of CRISPR technologies and their applications in plants, from the initial introduction of random small indel (insertion or deletion) mutations at target genomic loci to precision editing such as base editing, prime editing and gene targeting. We describe advances in the use of class 2, types II, V, and VI systems for gene disruption as well as for precise sequence alterations, gene transcription, and epigenome control.

journal_name

J Integr Plant Biol

authors

Zhan X,Lu Y,Zhu JK,Botella JR

doi

10.1111/jipb.13063

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

3-33

issue

1

eissn

1672-9072

issn

1744-7909

journal_volume

63

pub_type

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