Abstract：
:Gene expression studies are important for revealing gene functions putatively involved in biological processes. We were interested in identifying differentially expressed genes during leaf development in rice. We combined the RNA arbitrarily primed-polymerase chain reaction (RAP-PCR) and dot blot hybridization methods to screen a rice leaf primordium cDNA library. Three developmental stages during vegetative growth were examined. The cDNA clones showing different hybridization patterns were further analyzed and verified. Here we demonstrate that the combination of RAP-PCR and dot blot hybridization could provide an efficient and relatively low-cost cDNA library screening approach to discover genes not previously known to be associated with leaf development in rice. We believe that the findings described here will help to elucidate the molecular mechanism(s) underlying the developmental processes of rice leaf.
journal_name
J Integr Plant Bioljournal_title
Journal of integrative plant biologyauthors
Fung YW,Chow HY,Law TW,Dong B,Kwan HSdoi
10.1111/j.1744-7909.2008.00801.xsubject
Has Abstractpub_date
2009-06-01 00:00:00pages
614-21issue
6eissn
1672-9072issn
1744-7909pii
JIPB801journal_volume
51pub_type
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