Abstract:
:High-quality and disease-resistant male sterile lines have great potential for applications in hybrid rice breeding. We introduced specific mutations into the TMS5, Pi21, and Xa13 genes in Pinzhan intermediate breeding material using the CRISPR/Cas9 multiplex genome editing system. We found that the transgene-free homozygous triple tms5/pi21/xa13 mutants obtained in the T1 generation displayed characteristics of thermosensitive genic male sterility (TGMS) with enhanced resistance to rice blast and bacterial blight. Our study provides a convenient and effective way of converting breeding intermediate material into TGMS lines through multiplex gene editing, which could significantly accelerate the breeding of sterile lines.
journal_name
J Integr Plant Bioljournal_title
Journal of integrative plant biologyauthors
Li S,Shen L,Hu P,Liu Q,Zhu X,Qian Q,Wang K,Wang Ydoi
10.1111/jipb.12774subject
Has Abstractpub_date
2019-12-01 00:00:00pages
1201-1205issue
12eissn
1672-9072issn
1744-7909journal_volume
61pub_type
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