Abstract:
:Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches: (i) AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain (GVG) chemical-inducible expression system; (ii) deletion of AcTPase via Cre-lox site-specific recombination that was initially triggered by Ds excision; and (iii) suppression of early transposition events in transformed rice callus through a dual-functional hygromycin resistance gene in a novel Ds element (HPT-Ds). We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the community.
journal_name
J Integr Plant Bioljournal_title
Journal of integrative plant biologyauthors
Qu S,Jeon JS,Ouwerkerk PB,Bellizzi M,Leach J,Ronald P,Wang GLdoi
10.1111/j.1744-7909.2009.00870.xsubject
Has Abstractpub_date
2009-11-01 00:00:00pages
982-92issue
11eissn
1672-9072issn
1744-7909pii
JIPB870journal_volume
51pub_type
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