Crossability barriers in the interspecific hybridization between Oryza sativa and O. meyeriana.

Abstract:

:Oryza meyeriana Baill (GG genome) is a precious germplasm in the tertiary gene pool of cultivated rice (AA genome), and possesses important traits such as resistance and tolerance to biotic and abiotic stress. However, interspecific crossability barrier, a critical bottleneck restricting genes transfer from O. meyeriana to cultivars has led to no hybrids through conventional reproduction. Therefore, the reasons underlying incrossability were investigated in the present report. The results showed that: (i) at 3-7 d after pollination (DAP), many hybrid embryos degenerated at the earlier globular-shaped stage, and could not develop into the later pear-shaped stage. Meanwhile, free endosperm nuclei started to degenerate at 1 DAP, and cellular endosperm could not form at 3 DAP, leading to nutrition starvation for young embryo development; (ii) at 11-13 DAP, almost all hybrid ovaries aborted. Even though 72.22% of hybrid young embryos were produced in the interspecific hybridization between O. sativa and O. meyeriana, young embryos were not able to further develop into hybrid plantlets via culturing in vitro. The main reason for the incrossability was hybrid embryo inviability, presenting as embryo development stagnation and degeneration since 3 DAP. Some possible approaches to overcome the crossability barriers in the interspecific hybridization between O. sativa and O. meyeriana are discussed.

journal_name

J Integr Plant Biol

authors

Fu XL,Lu YG,Liu XD,Li JQ

doi

10.1111/j.1744-7909.2008.00728.x

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

21-8

issue

1

eissn

1672-9072

issn

1744-7909

pii

JIPB728

journal_volume

51

pub_type

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