Characterizing excited conformational states of RNA by NMR spectroscopy.

Abstract:

:Conformational dynamics is a hallmark of diverse non-coding RNA functions. During these functional processes, RNA molecules almost ubiquitously undergo conformational transitions that are tuned to meet distinct structural and kinetic requirements for proper function. A complete mechanistic understanding of RNA function requires comprehensive structural and dynamic knowledge of these complex transitions, which often involve alternative higher-energy conformational states that pose a major challenge for high-resolution structural study by conventional methods. In this review, we describe recent progress in RNA NMR that has started to unveil detailed structural, thermodynamic and kinetic insights into some of these excited conformational states of RNA and their functional roles in biology.

journal_name

Curr Opin Struct Biol

authors

Zhao B,Zhang Q

doi

10.1016/j.sbi.2015.02.011

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

134-146

eissn

0959-440X

issn

1879-033X

pii

S0959-440X(15)00020-2

journal_volume

30

pub_type

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