Abstract:
:Pulmonary surfactant proteins SP-B and SP-C were isolated from lavage fluids of bovine lungs and recombined (lipid/proteins, 9/1, wt/wt) with dipalmitoyl phosphatidylcholine for testing in vitro and in surfactant-deficient adult rats. Using a pulsating bubble surfactometer, we found that inflation pressures of bubbles at minimum radii in these mixtures were 0.34 +/- 0.05 cm H2O (+/- SD, n = 24) after 1 min. These values were not affected by increasing amounts of surfactant protein relative to dipalmitoyl phosphatidylcholine (DPPC). Minimum inflation pressures were similar to those of modified bovine surfactant, surfactant Tokyo Akita (TA) (0.33 +/- 0.05 cm H2O, n = 7). In vivo testing was carried out in adult rats made surfactant deficient by repeated lavage and ventilated with 100% oxygen. Rats received tracheal instillations of either air, DPPC, DPPC/SP-B,C (9:1), or surfactant TA at 50 mg/kg body weight. Surfactant TA and DPPC/SP-B, SP-C mixtures resulted in similar immediate and sustained improvements in arterial oxygenation (308 +/- 66 torr, n = 10 and 312 +/- 101 torr, n = 6 at 30 min posttreatment) that were significantly greater than those of sham (76 +/- 24 torr, n = 17) and DPPC-treated rats (64 +/- 32 torr, n = 7). Rats treated with either DPPC/SP-B,C mixtures or surfactant TA showed similar postmortem static lung compliances (2.3 +/- 0.8 ml/cm H2O/kg, n = 8 and 1.9 +/- 0.4 ml/cm H2O/kg, n = 5, respectively) that were significantly larger than sham (1.3 +/- 0.3 ml/cm H2O/kg, n = 14) and DPPC-treated rats (1.2 +/- 0.2 ml/cm H2O/kg, n = 6).(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Smith GB,Taeusch HW,Phelps DS,Keough KMdoi
10.1203/00006450-198805000-00010subject
Has Abstractpub_date
1988-05-01 00:00:00pages
484-90issue
5eissn
0031-3998issn
1530-0447journal_volume
23pub_type
杂志文章abstract::To elucidate the impact of nutrition in cystic fibrosis (CF), we compared the phenotypic traits of Cftr -/- mice fed either a lipid-enriched liquid diet (Peptamen) or a standard chow combined with polyethylenglycol osmotic laxative (PEG), two strategies commonly used to prevent intestinal obstruction in CF mice. Survi...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e318155a61d
更新日期:2007-11-01 00:00:00
abstract::We have identified two unrelated girls with chronic neutropenia [absolute neutrophil counts (ANC) 10-870 and 10-940/microL in patients 1 and 2, respectively] and severe defect in superoxide anion generation by granulocytes. Formyl-methionyl-leucyl-phenylalanine-induced superoxide release was 1.2 +/- 0.9 and 1.9 +/- 1....
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199501000-00011
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND:Previous studies indicate an increased risk for neuropsychological difficulties in young children prenatally exposed to opioids and polysubstances, but longitudinal information is scarce. The present longitudinal study investigated whether these waned, persisted, or increased over time. METHODS:The cognitiv...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2015.95
更新日期:2015-09-01 00:00:00
abstract::We studied choline uptake by slices of adult and 10-day-old rat intestine which were exposed on their mucosal surface to radiolabeled choline. Both neonatal and adult intestine transported choline. Choline uptake was observed in duodenum, jejunum, ileum, and colon of the adult rat. In the small intestine, choline upta...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198608000-00014
更新日期:1986-08-01 00:00:00
abstract::A four-year-old Israeli Arab boy was found to have glycogen accumulation in both liver and muscle without clinical symptoms. Liver phosphorylase kinase (PK) activity was 20% of normal, resulting in undetectable activity of phosphorylase a. Muscle PK activity was about 25% of normal, resulting in a marked decrease of p...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198104000-00002
更新日期:1981-04-01 00:00:00
abstract::Very young children with organic brain damage, intractable seizures, and developmental retardation are at particular risk of developing fatal hepatic dysfunction coincident with valproate therapy, especially if the children are also receiving other anticonvulsant drugs. The mechanism of valproate-associated hepatic fa...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199204000-00023
更新日期:1992-04-01 00:00:00
abstract::Immunosuppressive effects of carrageenan, a high-molecular-weight polysaccharide, on antibody and T cell responses have been previously demonstrated. However, its effect on anaphylaxis is unknown. Our objectives were to test carrageenan-mediated oral tolerance induction in young mice subsequently sensitized to a commo...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200103000-00018
更新日期:2001-03-01 00:00:00
abstract::The objective of this study was to obtain insight into bone formation and resorption in children with newly diagnosed untreated acute lymphoblastic leukemia (ALL). In 23 consecutive children with ALL, a bone biopsy was taken from the crista iliaca posterior under ketamine anesthesia, together with the diagnostic marro...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000090929.16450.DE
更新日期:2003-12-01 00:00:00
abstract::Chondro-osseous tissue from five patients with diastropic dwarfism was studied by histologic, histochemical, and electron microscopic methods. The major abnormalities observed were: 1) irregular distribution of chondrocytes undergoing degeneration in the resting cartilage; 2) abnormal distribution of collagens in the ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197908000-00007
更新日期:1979-08-01 00:00:00
abstract::Near-infrared spectroscopy is a noninvasive method for monitoring brain oxygenation. The aim of the study was to investigate differences between cerebral oxygenation in different brain regions in newborns. In a prospective study, we monitored simultaneously left and right frontoparietal and temporo-occipital regional ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e31822a36db
更新日期:2011-10-01 00:00:00
abstract::Phototherapy results in the conversion of bilirubin to more water-soluble isomers. Six clinically used phototherapy lamps which differ in their emission spectra have been compared in their ability to produce configurational and structural isomers of bilirubin in vitro. For all of the lamps, the initial rate of configu...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198407000-00021
更新日期:1984-07-01 00:00:00
abstract::We analyzed by means of immunoblot technique the patterns of antibodies binding to polypeptides of Borrelia burgdorferi B31 in the sera of 21 children with different stages of Lyme disease. All sera but one recognized the flagellar protein 41 kD and all but two the 83-kD protein. The number of proteins recognized rose...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198910000-00019
更新日期:1989-10-01 00:00:00
abstract::To determine the effects of age, feeding regimen, and antenatal glucocorticoids on intestinal permeability, preterm infants (n = 132) were stratified by gestational age and by diet (mothers' own milk versus preterm formula), and assigned randomly to one of four feeding regimens: early-continuous, early-bolus, standard...
journal_title:Pediatric research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1203/00006450-199810000-00009
更新日期:1998-10-01 00:00:00
abstract:BACKGROUND:Adolescents born preterm have altered hypothalamic-pituitary-adrenal axis function with a blunted cortisol stress response, however, the influences of intrauterine growth restriction and race are unclear. METHODS:We measured salivary cortisol before and 20 min after a maximal-exercise stress test and calcul...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-019-0682-3
更新日期:2020-05-01 00:00:00
abstract::The primary manifestations of Pompe disease are muscle weakness and cardiomyopathy. Although accumulation of glycogen has also been seen in the nervous system in patients, the significance of brain involvement in infantile-onset Pompe disease is not clear. In this study, brain development in five cases of infantile-on...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000233014.84318.4e
更新日期:2006-09-01 00:00:00
abstract::The flavin-containing monooxygenases (FMOs) are important for the metabolism of numerous therapeutics and toxicants. Six mammalian FMO genes (FMO1-6) have been identified, each exhibiting developmental and tissue- and species-specific expression patterns. Previous studies demonstrated that human hepatic FMO1 is restri...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200202000-00018
更新日期:2002-02-01 00:00:00
abstract:BACKGROUND:Hirschsprung disease (HSCR) is the third most common congenital disorder of the gastrointestinal tract. This study aims to elucidate changes in protein expression between the normal and aganglionic hindgut in human HSCR. METHODS:The biopsies were obtained from the normal and aganglionic hindgut in human HSC...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2014.33
更新日期:2014-06-01 00:00:00
abstract::We determined whether closing the ductus arteriosus of the fetal lamb several d before birth would cause persistent pulmonary hypertension after birth. Six experimental fetuses who had their ductus arteriosus ligated 3-17 d before delivery, three control fetuses who had sham ligation of the ductus arteriosus 14 d befo...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198903000-00005
更新日期:1989-03-01 00:00:00
abstract::Spontaneous preterm birth is a serious and common pregnancy complication associated with hormonal dysregulation, infection, inflammation, immunity, rupture of fetal membranes, stress, bleeding, and uterine distention. Heredity is 25-40% and mostly involves the maternal genome, with contribution of the fetal genome. Si...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/s41390-018-0180-z
更新日期:2019-03-01 00:00:00
abstract::Astrocyte end-feet ensheathe blood vessels in the brain and are believed to provide structural integrity to the cerebral vasculature. We sought to determine in developing infants whether the coverage of blood vessels by astrocyte end-feet is decreased in germinal matrix (GM) compared with cerebral cortex and white mat...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000214975.85311.9c
更新日期:2006-05-01 00:00:00
abstract::Sequential magnitude and the rate of change in tracheal mechanics after application of intermittent positive pressure (IPP), and the time constant, tau, of this deformation were determined in fetal (21 and 27 days gestation), neonatal (term, 31 days gestation) and adult rabbits. In vitro tracheal mechanics were determ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198210000-00006
更新日期:1982-10-01 00:00:00
abstract::We assessed the patterns of Helicobacter pylori (H. pylori) specific maternal antibodies in maternal serum, cord blood, and milk, which might play a role in prevention of H. pylori infection because transferred to the infant. Between November 2000 and November 2001, mothers were recruited after delivery of their offsp...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000181370.67474.FD
更新日期:2005-11-01 00:00:00
abstract::Wu and colleagues analyzed the placental pathology from a subset of the neonates in the NEATO trial who had reports available and correlated the placental pathology findings with outcomes. This study highlights the importance of placental pathology, and its potential to bring precision medicine to critically-ill neona...
journal_title:Pediatric research
pub_type: 评论,杂志文章
doi:10.1038/s41390-020-0807-8
更新日期:2020-04-01 00:00:00
abstract::To study the course of regional metabolite concentrations during early brain development, we measured in vivo metabolites [N-acetyl-aspartate (NAA), choline-containing compounds, and myoinositol (M-Ino)] in the precentral area of the cerebrum by short echo-time single volume proton magnetic resonance spectroscopy and ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199502000-00003
更新日期:1995-02-01 00:00:00
abstract::Cell-mediated immunity is not well characterized in very low birth weight infants, and abnormalities may represent a significant vulnerability to infection. This report describes 165 serial studies in 58 infants between 700 and 1300 g birth weight during the first 8 wk of life. Two ml of blood were drawn at 2-wk inter...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198805000-00003
更新日期:1988-05-01 00:00:00
abstract::Intrauterine growth restriction (IUGR) is a recognized risk factor for neurologic deficits later in life. Abnormal fetal blood flow in the presence of fetal growth retardation helps to distinguish true fetal growth impairment from small but normally grown infants. The present study aimed to investigate the influence o...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000129660.32875.09
更新日期:2004-07-01 00:00:00
abstract::Surfactant-associated proteins (SP-A, SP-B, and SP-C) are critical for the endogenous function of surfactant. Keratinocyte growth factor (KGF) and vascular endothelial growth factor (VEGF) are key regulators of lung development. The objective of this study was to evaluate the effects of early mechanical ventilation on...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181256aeb
更新日期:2007-09-01 00:00:00
abstract::Gluconeogenesis decreases during septic shock, but its mechanism is not well known. Tumor necrosis factor alpha (TNF-alpha), which is a key cytokine in septic shock, can increase GLUT1 gene expression and glucose uptake in muscles and fatty tissues. TNF-alpha does not alter the metabolism of hepatocytes in which GLUT2...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200104000-00018
更新日期:2001-04-01 00:00:00
abstract::The interactions between CD40 and CD40L (CD154) are critical for effective humoral immune response. CD40 signaling facilitates T lymphocyte dependent B cell proliferation and immunoglobulin isotype switch. The objective of our study was to investigate the CD40 and CD40L expression on peripheral blood mononuclear cells...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000219298.96471.18
更新日期:2006-06-01 00:00:00
abstract::Indomethacin, because of its prostaglandin inhibition, may cause constriction of the ductus arteriosus and affect the pulmonary circulation. To study this possibility, we gave indomethacin daily by gavage to two groups of pregnant rats from the 17th day of pregnancy through delivery (group 1, 2 mg/kg/day; group 2, 4 m...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198102000-00013
更新日期:1981-02-01 00:00:00