Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates by a phaZ-knockout strain of Pseudomonas putida KT2440.


:A medium-chain-length poly-3-hydroxyalkanote (MCL-PHA) depolymerase knockout mutant of Pseudomonas putida KT2440 was produced by double homologous recombination. A carbon-limited shake-flask study confirmed that depolymerase activity was eliminated. Lysis of both mutant and wild-type strains occurred under these conditions. In carbon-limited, fed-batch culture, the yield of unsaturated monomers from unsaturated substrate averaged only 0.62 mol mol(-1) for the phaZ minus strain compared to 0.72 mol mol(-1) for the wild type. The mutant strain also produced more CO2 and less residual biomass from the same amount of carbon substrate. However, most results indicated that elimination of PHA depolymerase activity had little impact on the overall yield of biomass and PHA.


Vo MT,Ko K,Ramsay B




Has Abstract


2015-04-01 00:00:00












  • Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1.

    abstract::Pseudomonas aeruginosa UW-1 produced 17-24 g L-1 rhamnolipid in vegetable oil-containing media in shake flask cultures in 13 days. In time course studies of growth and rhamnolipid production in a salts medium containing 6% canola oil, total bacterial count reached 2.6 x 10(10) CFU ml-1 after 48 h and a maximum rhamnol...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Sim L,Ward OP,Li ZY

    更新日期:1997-10-01 00:00:00

  • Electrochemical behavior of the 316L steel type in a marine culture of microalgae (Porphyridium purpureum) under the 12/12 h photoperiod and effect of different working electrode exposure conditions on the biofilm-metal interface.

    abstract::The industrial crops of microalgae use processes calling upon the presence of parts of metal nature such as steel 316L type. The goal of this study is to test the electrochemical behavior of this material in a marine culture of microalgae. Porphyridium purpureum was used under a photoperiod of alternation darkness/lig...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Djemai-Zoghlache Y,Isambert A,Belhaneche-Bensemra N

    更新日期:2011-12-01 00:00:00

  • L(+)-lactic acid production by co-fermentation of glucose and xylose with Rhizopus oryzae obtained by low-energy ion beam irradiation.

    abstract::Low-energy ion beam irradiation (10-200 keV) has been proved to have a wide range of biological effects in recent years. When Rhizopus oryzae PW352 was irradiated with a 15-keV low-energy ion beam an L(+)-lactic acid high-yield mutant, RQ4015, was obtained. When 150 g/l glucose was used as the sole carbon source, L(+)...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Wang P,Li J,Wang L,Tang ML,Yu ZL,Zheng ZM

    更新日期:2009-11-01 00:00:00

  • Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars.

    abstract::Recombinant Escherichia coli have been constructed for the conversion of glucose as well as pentose sugars into L-lactic acid. The strains carry the lactate dehydrogenase gene from Streptococcus bovis on a low copy number plasmid for production of L-lactate. Three E. coli strains were transformed with the plasmid for ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Dien BS,Nichols NN,Bothast RJ

    更新日期:2001-10-01 00:00:00

  • Isolation and characterization of a biosurfactant producing strain, Brevibacilis brevis HOB1.

    abstract::Biosurfactant-producing bacteria were isolated from the production water of an oil field. Isolates were screened for biosurfactant production using surface tension test. The highest reduction of surface tension was achieved with a bacterial strain which was identified by 16S rRNA gene sequencing as Brevibacilis brevis...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Haddad NI,Wang J,Mu B

    更新日期:2008-12-01 00:00:00

  • Enhanced thermostability of keratinase by computational design and empirical mutation.

    abstract::Keratinases are proteolytic enzymes capable of degrading insoluble keratins. The importance of these enzymes is being increasingly recognized in fields as diverse as animal feed production, textile processing, detergent formulation, leather manufacture, and medicine. To enhance the thermostability of Bacillus lichenif...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Liu B,Zhang J,Fang Z,Gu L,Liao X,Du G,Chen J

    更新日期:2013-07-01 00:00:00

  • Assessment of physiological state of microorganisms in activated sludge with flow cytometry: application for monitoring sludge production minimization.

    abstract::Many sludge reduction processes have been studied for the minimization of sludge production in biological wastewater treatment. The investigations on most of these processes have monitored the increase of the soluble chemical oxygen demand, the sludge mass reduction, or the decrease of the floc size, but little inform...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Prorot A,Eskicioglu C,Droste R,Dagot C,Leprat P

    更新日期:2008-11-01 00:00:00

  • Synthesis of 4-nitrophenyl acetate using molecular sieve-immobilized lipase from Bacillus coagulans.

    abstract::Extracellular lipase from Bacillus coagulans BTS-3 was immobilized on (3 A x 1.5 mm) molecular sieve. The molecular sieve showed approximately 68.48% binding efficiency for lipase (specific activity 55 IU mg(-1)). The immobilized enzyme achieved approx 90% conversion of acetic acid and 4-nitrophenol (100 mM each) into...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Raghuvanshi S,Gupta R

    更新日期:2009-03-01 00:00:00

  • Tobermolite effects on methane removal activity and microbial community of a lab-scale soil biocover.

    abstract::Three identical lab-scale biocovers were packed with an engineered soil (BC 1), tobermolite only (BC 2), and a mixture of the soil and tobermolite (BC 3), and were operated at an inlet load of 338-400 g-CH4 m(-2) d(-1) and a space velocity of 0.12 h(-1). The methane removal capacity was 293 ± 47 g-CH4 m(-2) d(-1) in s...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Moon KE,Lee EH,Kim TG,Cho KS

    更新日期:2014-07-01 00:00:00

  • Extracellular electron uptake by autotrophic microbes: physiological, ecological, and evolutionary implications.

    abstract::Microbes exchange electrons with their extracellular environment via direct or indirect means. This exchange is bidirectional and supports essential microbial oxidation-reduction processes, such as respiration and photosynthesis. The microbial capacity to use electrons from insoluble electron donors, such as redox-act...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Gupta D,Guzman MS,Bose A

    更新日期:2020-10-01 00:00:00

  • Purification and characterization of an organic-solvent-tolerant cellulase from a halotolerant isolate, Bacillus sp. L1.

    abstract::A halotolerant isolate Bacillus sp. L1 producing extracellular cellulase was isolated from Yuncheng, China. Production of the enzyme started from mid-exponential phase of bacterial growth and reached a maximum level during the post-stationary phase. The cellulase was purified to homogeneity with molecular mass of 45 k...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Li X,Yu HY

    更新日期:2012-08-01 00:00:00

  • Novel organization of catechol meta pathway genes in the nitrobenzene degrader Comamonas sp. JS765 and its evolutionary implication.

    abstract::The catechol meta cleavage pathway is one of the central metabolic pathways for the degradation of aromatic compounds. A novel organization of the pathway genes, different from that of classical soil microorganisms, has been observed in Sphingomonas sp HV3 and Pseudomonas sp. DJ77. In a Comamonas sp. JS765, cdoE encod...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: He Z,Parales RE,Spain JC,Johnson GR

    更新日期:2007-02-01 00:00:00

  • A recombinant vaccine against hydatidosis: production of the antigen in Escherichia coli.

    abstract::A commercial process was developed for producing a recombinant vaccine against hydatidosis in farm animals. The vaccine antigen consisting of a surface protein of the oncospheres of the hydatid worm (Echinococcus granulosus), was produced as inclusion bodies in Escherichia coli. Fed-batch cultures of E. coli using Ter...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Manderson D,Dempster R,Chisti Y

    更新日期:2006-03-01 00:00:00

  • Identification of oleaginous yeasts that metabolize aromatic compounds.

    abstract::The valorization of lignin is critical for the economic viability of the bioeconomy. Microbial metabolism is advantageous for handling the myriad of aromatic compounds resulting from lignin chemical or enzymatic depolymerization. Coupling aromatic metabolism to fatty acid biosynthesis makes possible the production of ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Yaguchi A,Franaszek N,O'Neill K,Lee S,Sitepu I,Boundy-Mills K,Blenner M

    更新日期:2020-10-01 00:00:00

  • Dynamic metabolic control: towards precision engineering of metabolism.

    abstract::Advances in metabolic engineering have led to the synthesis of a wide variety of valuable chemicals in microorganisms. The key to commercializing these processes is the improvement of titer, productivity, yield, and robustness. Traditional approaches to enhancing production use the "push-pull-block" strategy that modu...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Liu D,Mannan AA,Han Y,Oyarzún DA,Zhang F

    更新日期:2018-07-01 00:00:00

  • Production of raw cassava starch-degrading enzyme by Penicillium and its use in conversion of raw cassava flour to ethanol.

    abstract::A newly isolated strain Penicillium sp. GXU20 produced a raw starch-degrading enzyme which showed optimum activity towards raw cassava starch at pH 4.5 and 50 °C. Maximum raw cassava starch-degrading enzyme (RCSDE) activity of 20 U/ml was achieved when GXU20 was cultivated under optimized conditions using wheat bran (...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Lin HJ,Xian L,Zhang QJ,Luo XM,Xu QS,Yang Q,Duan CJ,Liu JL,Tang JL,Feng JX

    更新日期:2011-06-01 00:00:00

  • Rapid and stable production of 2,3-butanediol by an engineered Saccharomyces cerevisiae strain in a continuous airlift bioreactor.

    abstract::Utilization of renewable feedstocks for the production of bio-based bulk chemicals, such as 2,3-butanediol (2,3-BDO), by engineered strains of the non-pathogenic yeast, Saccharomyces cerevisiae, has recently become an attractive option. In this study, to realize rapid production of 2,3-BDO, a flocculent, 2,3-BDO-produ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Yamada R,Nishikawa R,Wakita K,Ogino H

    更新日期:2018-05-01 00:00:00

  • Thermophilic aerobic treatment of a synthetic wastewater in a membrane-coupled bioreactor.

    abstract::Synthetic wastewater containing alpha-lactose and gelatin was treated in a thermophilic membrane-coupled bioreactor (MBR). Thermophilic (>45 degrees C) treatment represents a potentially advantageous process for high-temperature as well as high-strength industrial wastewaters susceptible to reactor autoheating. Thermo...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: LaPara TM,Konopka A,Nakatsu CH,Alleman JE

    更新日期:2001-04-01 00:00:00

  • Regioselective aromatic hydroxylation of quinaldine by water using quinaldine 4-oxidase in recombinant Pseudomonas putida.

    abstract::Biocatalytic hydrocarbon oxyfunctionalizations are typically accomplished using oxygenases in living bacteria as biocatalysts. These processes are often limited by either oxygen mass transfer, cofactor regeneration, and/or enzyme instabilities due to the formation of reactive oxygen species. Here, we discuss an altern...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Utkür FO,Gaykawad S,Bühler B,Schmid A

    更新日期:2011-08-01 00:00:00

  • Genetic switches and related tools for controlling gene expression and electrical outputs of Geobacter sulfurreducens.

    abstract::Physiological studies and biotechnology applications of Geobacter species have been limited by a lack of genetic tools. Therefore, potential additional molecular strategies for controlling metabolism were explored. When the gene for citrate synthase, or acetyl-CoA transferase, was placed under the control of a LacI/IP...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ueki T,Nevin KP,Woodard TL,Lovley DR

    更新日期:2016-11-01 00:00:00

  • Discovery of a new diol-containing polyketide by heterologous expression of a silent biosynthetic gene cluster from Streptomyces lavendulae FRI-5.

    abstract::The genome of streptomycetes has the ability to produce many novel and potentially useful bioactive compounds, but most of which are not produced under standard laboratory cultivation conditions and are referred to as silent/cryptic secondary metabolites. Streptomyces lavendulae FRI-5 produces several types of bioacti...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Pait IGU,Kitani S,Roslan FW,Ulanova D,Arai M,Ikeda H,Nihira T

    更新日期:2018-02-01 00:00:00

  • Engineering of isoamylase: improvement of protein stability and catalytic efficiency through semi-rational design.

    abstract::Isoamylase catalyzes the hydrolysis of α-1,6-glycosidic linkages in glycogen, amylopectin and α/β-limit dextrins. A semi-rational design strategy was performed to improve catalytic properties of isoamylase from Bacillus lentus. Three residues in vicinity of the essential residues, Arg505, Asn513, and Gly608, were chos...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Li Y,Zhang L,Ding Z,Gu Z,Shi G

    更新日期:2016-01-01 00:00:00

  • Whole cell microbial transformation in cloud point system.

    abstract::Cloud point system, consisting of nonionic surfactant in an aqueous solution, has been developed as a novel medium for whole cell microbial transformation. The basic properties of cloud point system including phase separation and solubilization are introduced. The application of cloud point system for extractive micro...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Wang Z,Xu JH,Chen D

    更新日期:2008-07-01 00:00:00

  • Comparative studies on extracellular protease secretion and glucoamylase production by free and immobilized Aspergillus niger cultures.

    abstract::The effects of cell immobilization on the secretion of extracellular proteases and glucoamylase production by Aspergillus niger were investigated under a variety of immobilization techniques and culture conditions. Immobilization was achieved by means of cell attachment on metal surfaces or spore entrapment and subseq...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Papagianni M,Joshi N,Moo-Young M

    更新日期:2002-11-01 00:00:00

  • Effects of lipidic carbon sources on the extracellular lipolytic activity of a newly isolated strain of Bacillus subtilis.

    abstract::An isolate exhibiting high extracellular lipolytic activity was identified as Bacillus subtilis by 16S rRNA gene sequence analysis. The enzyme activity of the isolate was improved by using different concentrations of lipidic carbon sources such as vegetable oils, fatty acids and triglycerides. Lipolytic activity was a...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Takaç S,Marul B

    更新日期:2008-09-01 00:00:00

  • Strain improvement for fermentation and biocatalysis processes by genetic engineering technology.

    abstract::Twenty years ago, the first complete gene cluster encoding the actinorhodin biosynthetic pathway was cloned and characterized. Subsequently, the gene clusters encoding the biosynthetic pathways for many antibiotics were isolated. In the past decade, breakthroughs in technology brought that generation of rationally des...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Chiang SJ

    更新日期:2004-03-01 00:00:00

  • Comparative analysis of the Corynebacterium glutamicum transcriptome in response to changes in dissolved oxygen levels.

    abstract::The dissolved oxygen (DO) level of a culture of Corynebacterium glutamicum (C. glutamicum) in a bioreactor has a significant impact on the cellular redox potential and the distribution of energy and metabolites. In this study, to gain a deeper understanding of the effects of DO on the metabolism of C. glutamicum, we s...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,收录出版


    authors: Liu X,Yang S,Wang F,Dai X,Yang Y,Bai Z

    更新日期:2017-02-01 00:00:00

  • Influence of straw types and nitrogen sources on mushroom composting emissions and compost productivity.

    abstract::The effects of different straw types and organic and inorganic nitrogen (N) sources on the chemical composition and odor concentration (OC) of mushroom composting emissions, compost parameters, and mushroom yield were examined using bench-scale and large-scale (windrows and aerated tunnels) composting systems. There w...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Noble R,Hobbs PJ,Mead A,Dobrovin-Pennington A

    更新日期:2002-09-01 00:00:00

  • Recombinant carbazole-degrading strains for enhanced petroleum processing.

    abstract::Biotechnological upgrading of fossil fuels is of increasing interest as remaining stocks of petroleum show increasing levels of contaminants such as heavy metals, sulfur and nitrogen-containing heteroaromatic compounds. Carbazole is of particular interest as a major petroleum component known to reduce refining yields ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Riddle RR,Gibbs PR,Willson RC,Benedik MJ

    更新日期:2003-01-01 00:00:00

  • Evaluation of the catalytic specificity, biochemical properties, and milk clotting abilities of an aspartic peptidase from Rhizomucor miehei.

    abstract::In this study, we detail the specificity of an aspartic peptidase from Rhizomucor miehei and evaluate the effects of this peptidase on clotting milk using the peptide sequence of k-casein (Abz-LSFMAIQ-EDDnp) and milk powder. Molecular mass of the peptidase was estimated at 37 kDa, and optimum activity was achieved at ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: da Silva RR,Souto TB,de Oliveira TB,de Oliveira LC,Karcher D,Juliano MA,Juliano L,de Oliveira AH,Rodrigues A,Rosa JC,Cabral H

    更新日期:2016-08-01 00:00:00