Influence of intravenous nutrients on bilirubin transport. II. Emulsified lipid solutions.

Abstract:

:The effects of an emulsified intravenous fat preparation (Intralipid) on bilirubin transport were analyzed by cholestyramine extraction, spectrophotometric analysis, Sephadex gel filtration, peroxidase assay, bilirubin uptake by red blood cells, and by toxicity in tissue culture (L-929) cells. Intralipid is capable of binding bilirubin, but does not compete effectively with bilirubin bound to high affinity sites on albumin. The emulsified fat appears to have a higher affinity for bilirubin than for cell membranes. Red blood cells become coated with Intralipid, resulting in an increased association of bilirubin with the cell surface, but a decrease in pigment actually incorporated into the red cell membrane. In tissue culture experiments, Intralipid protected the cells from bilirubin toxicity. It is concluded that Intralipid may enhance the carrying capacity of serum for bilirubin, and thus exert a protective effect on tissues.

journal_name

Pediatr Res

journal_title

Pediatric research

authors

Thaler MM,Wennberg RP

doi

10.1203/00006450-197703000-00004

subject

Has Abstract

pub_date

1977-03-01 00:00:00

pages

167-71

issue

3 Pt 1

eissn

0031-3998

issn

1530-0447

journal_volume

11

pub_type

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