Abstract:
INTRODUCTION:Immobilization induced by experimental denervation leads to rapid and progressive alterations in structural and biochemical properties of skeletal muscle. Real-time reverse transcription-polymerase chain reaction (RT-PCR) is a popular method of elucidating the molecular mechanisms involved in muscle atrophy. Identification of suitable reference genes that are not affected by experimental conditions is a critical step in accurate normalization of real-time RT-PCR. METHODS:We investigated the impact of denervation-induced muscle atrophy for 2 weeks on the expression of common housekeeping genes. RESULTS:Denervation differentially affected the expression levels of these genes. RefFinder software identified TATA box binding protein (Tbp) as the most stable gene and showed that the stability of glyceraldehyde-3-phosphate dehydrogenase (Gapdh) and hypoxanthine guanine phosphoribosyl transferase (Hprt) genes was low, even though they are widely used for normalization. CONCLUSIONS:The appropriate reference gene for normalization of genes of interest in denervated muscle is Tbp.
journal_name
Muscle Nervejournal_title
Muscle & nerveauthors
Nakao R,Yamamoto S,Yasumoto Y,Kadota K,Oishi Kdoi
10.1002/mus.24310subject
Has Abstractpub_date
2015-02-01 00:00:00pages
276-81issue
2eissn
0148-639Xissn
1097-4598journal_volume
51pub_type
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