Abstract：
:By use of ion exchange chromatography we have isolated two discrete classes of "free" glycosaminoglycans (GAG) from human plasma. The GAG fractions were tested for their effects on two lipoprotein lipase (LPL) enzyme systems containing an apolipoprotein C-II activated emulsion as the triglyceride substrate and bovine serum albumin as the free fatty acid acceptor. The lowcharge GAG (Fraction I) had essentially no effect on the LPL reaction. The high-charge GAG (Fraction II) stimulated the LPL reaction 100 to 300%. The GAG composition of each fraction was investigated with chemical and enzymatic techniques. Fraction I consisted of low-charge chondroitin sulfate noncovalently bound to protein. Fraction II consisted of a mixture of high-charge GAG non-covalently bound to protein. Degradation with nitrous acid eliminated the ability of high-charge GAG to stimulate LPL. This and other evidence suggests that the high-charge GAG in human plasma responsible for LPL activation is heparan sulfate (HS). We suggest that plasma HS may modulate triglyceride clearance mechanisms in vivo by its interaction with LPL.
journal_name
Life Scijournal_title
Life sciencesauthors
Felts JM,Staprans I,Gorman RAdoi
10.1016/0024-3205(83)90874-3subject
Has Abstractpub_date
1983-04-04 00:00:00pages
1659-64issue
14eissn
0024-3205issn
1879-0631pii
0024-3205(83)90874-3journal_volume
32pub_type
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