Development and characterization of an IL-4-secreting human ovarian carcinoma cell line.


:Human ovarian carcinoma cell lines were genetically engineered to secrete the cytokine interleukin-4 (IL-4) by retroviral-mediated gene transduction. These cells were transduced with the LXSN retroviral vector containing the human IL-4 gene and the neomycin resistance selection marker. Numerous IL-4-secreting clones were isolated from different papillary serous carcinoma cell lines, including SKOV-3, UCI-101, and UCI-107, and one clone derived from UCI-107 extensively characterized. This clone, termed UCI 107E IL-4 GS, was shown to constitutively express high levels of IL-4 (i.e., 900 to 1300 pg/ml/10(5) cells/48 hr) for over 35 passages and 6 months of study. Like the parental cell line (UCI-107), UCI 107E IL-4 GS cells expressed MHC class I and Her-2/neu surface antigens but did not express detectable MHC class II, ICAM 1, CA 125, or IL-4 receptors. No increase in expression of surface proteins was noted between parental and UCI 107E IL-4 GS. The morphology of this clone did not differ from that of the parental or LXSN vector control cells; however, parental cells had a faster growth rates than transductants. UCI 107E IL-4 GS was sensitive to gamma irradiation since as little as 2500 rad killed most of the cells within 10 days of irradiation. However, after irradiation, IL-4 secretion continued until about Day 8. The potential use of these IL-4-secreting ovarian carcinoma cells as vaccines for woman with advanced ovarian cancer will be discussed.


Gynecol Oncol


Gynecologic oncology


Santin AD,Ioli GR,Hiserodt JC,Rose GS,Graf MR,Tocco LM,Lander JK,Eck LM,Burger RA,DiSaia PJ




Has Abstract,Author List Incomplete


1995-08-01 00:00:00














  • Absence of PTEN repeat tract mutation in endometrial cancers with microsatellite instability.

    abstract:OBJECTIVE:PTEN, a tumor suppressor gene shown to be frequently mutated in endometrial cancers, has been suggested to be a target of microsatellite instability (MSI)-driven mutagenesis. We set out to investigate the relationship between MSI and PTEN mutation in a large series of primary endometrial carcinomas. METHODS:...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Cohn DE,Basil JB,Venegoni AR,Mutch DG,Rader JS,Herzog TJ,Gersell DJ,Goodfellow PJ

    更新日期:2000-10-01 00:00:00

  • HER2/neu gene amplification determines the sensitivity of uterine serous carcinoma cell lines to AZD8055, a novel dual mTORC1/2 inhibitor.

    abstract:OBJECTIVE:To evaluate c-erbB2 gene amplification in a series of primary uterine serous carcinoma (USC) cell lines. To assess the efficacy of AZD8055, a novel dual mTORC1/2 inhibitor against primary HER2/neu amplified vs HER2/neu not amplified USC cell lines. METHODS:Twenty-two primary USC cell lines were evaluated for...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: English DP,Roque DM,Carrara L,Lopez S,Bellone S,Cocco E,Bortolomai I,Schwartz PE,Rutherford T,Santin AD

    更新日期:2013-12-01 00:00:00

  • Primary biliary carcinoma with metastasis to the ovary.

    abstract::The ovary is a relatively frequent site of metastasis from malignant neoplasia arising elsewhere in the body, the majority of these originating from the GI tract. The best known tumor of this type is signet-ring cell adenocarcinoma (Krukenberg tumor) of gastric origin. The gall bladder and bile ducts are rare sources ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审


    authors: Lashgari M,Behmaram B,Hoffman JS,Garcia J

    更新日期:1992-11-01 00:00:00

  • Feasibility study comparing docetaxel-cisplatin versus docetaxel-carboplatin as first-line chemotherapy for ovarian cancer.

    abstract:OBJECTIVE:To determine the feasibility of docetaxel-cisplatin combination therapy compared with docetaxel-carboplatin combination therapy as first-line chemotherapy for patients with ovarian cancer. METHODS:Fifty patients with International Federation of Gynecology and Obstetrics stage Ic-IV ovarian cancer who underwe...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Minagawa Y,Kigawa J,Kanamori Y,Itamochi H,Terakawa N,Okada M,Kitada F

    更新日期:2006-06-01 00:00:00

  • Determinants of response to a cisplatin-based regimen as neoadjuvant chemotherapy in stage IB-IIB invasive cervical cancer.

    abstract::The response rate and the determinants of response to a cisplatin-based regimen of neodjuvant chemotherapy (NACT) in women with a histologically confirmed first-diagnosis of invasive bulky stage IB-IIB cervical cancer are analyzed. A total of 79 cases were treated with cisplatin (40 mg/m2) each 7 days for seven course...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章


    authors: Bolis G,van Zainten-Przybysz I,Scarfone G,Zanaboni F,Scarabelli C,Tateo S,Calabrese M,Parazzini F

    更新日期:1996-10-01 00:00:00

  • Sensitivity to Fas-mediated apoptosis in high-risk HPV-positive human cervical cancer cells: relationship with Fas, caspase-8, and Bid.

    abstract:OBJECTIVE:Binding of Fas ligand or agonistic anti-Fas antibody to the death receptor Fas can activate a caspase-cascade resulting in apoptosis. In the present study, the functionality of the Fas pathway was studied in human cervical cancer cells with different HPV and p53 status. METHODS:HeLa (HPV-18 positive), CaSki,...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Hougardy BM,van der Zee AG,van den Heuvel FA,Timmer T,de Vries EG,de Jong S

    更新日期:2005-05-01 00:00:00

  • Laminin C1 expression by uterine carcinoma cells is associated with tumor progression.

    abstract:OBJECTIVES:Molecular markers associated with tumor progression in uterine carcinoma are poorly defined. In this study, we determine whether upregulation of LAMC1, a gene encoding extracellular matrix protein, laminin γ1, is associated with various uterine carcinoma subtypes and stages of tumor progression. METHODS:An ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Kashima H,Wu RC,Wang Y,Sinno AK,Miyamoto T,Shiozawa T,Wang TL,Fader AN,Shih IeM

    更新日期:2015-11-01 00:00:00

  • Endometrial cancer presentation is associated with social determinants of health in a public healthcare system: A population-based cohort study.

    abstract:OBJECTIVE:Delay in diagnosis of endometrial cancer may be associated with disease progression and impact management and outcomes. Social and cultural barriers influence recognition of symptoms and self-advocacy in seeking care. Associations between social determinants of health (SDH) and disease presentation have been ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Helpman L,Pond GR,Elit L,Anderson LN,Seow H

    更新日期:2020-07-01 00:00:00

  • The benefits of low anterior en bloc resection as part of cytoreductive surgery for advanced primary and recurrent epithelial ovarian cancer patients outweigh morbidity concerns.

    abstract:OBJECTIVES:The aim of this study was to assess the safety, efficacy and impact on survival of low anterior resection and primary anastomosis at the time of en bloc resection for primary and recurrent epithelial ovarian carcinoma. METHODS:We performed a retrospective review of 46 primary and 14 recurrent epithelial ova...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Park JY,Seo SS,Kang S,Lee KB,Lim SY,Choi HS,Park SY

    更新日期:2006-12-01 00:00:00

  • Association between high risk papillomavirus DNA and nitric oxide release in the human uterine cervix.

    abstract:OBJECTIVE:Local cervical factors may determine the outcome of human papillomavirus (HPV) infection. Nitric oxide (NO) may be one such factor, since it is produced by uterine cervical cells and it takes part in both immunological and carcinogenic reactions. We studied the association between the presence of cervical hig...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Rahkola P,Mikkola TS,Ylikorkala O,Vaisanen-Tommiska M

    更新日期:2009-08-01 00:00:00

  • Laparoscopically assisted Schauta operation: learning experience at the Gynecologic Oncology Unit, Buenos Aires University Hospital.

    abstract:OBJECTIVES:The aim of this study was to show the learning experience of the employment of laparoscopic lymphadenectomy followed by a Schauta operation to treat patients with cervical carcinoma at a university hospital and to evaluate the feasibility, complications, hospital stay, delay in return to work, and overall su...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Sardi J,Vidaurreta J,Bermúdez A,di Paola G

    更新日期:1999-12-01 00:00:00

  • Durability of clinical performance afforded by self-collected HPV testing: A 15-year cohort study in China.

    abstract:OBJECTIVE:Self-collected HPV testing could substantially reduce disparities in cervical cancer screening, with slightly lower sensitivity compared to physician-collected specimens cross-sectionally. We aimed to evaluate the comprehensive long-term performance of self-collected HPV testing prospectively. METHODS:In 199...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Zhang L,Xu XQ,Hu SY,Chen F,Zhang X,Pan QJ,Zhang WH,Feng RM,Dong L,Zhang Q,Ma JF,Sauvaget C,Zhao FH,Qiao YL,Sankaranarayanan R

    更新日期:2018-11-01 00:00:00

  • Analysis of common variations in tumor-suppressor genes on chr1p36 among Caucasian women with endometriosis.

    abstract:OBJECTIVE:Epidemiological data indicate that endometriosis increases the risk of epithelial ovarian cancer (EOC), but the mechanism of cancer transition is unknown. Results from genome-wide association studies (GWAS) and transcriptome sequencing have demonstrated that genes located in the 1p36 region are important in b...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Falconer H,Sundqvist J,Xu H,Vodolazkaia A,Fassbender A,Kyama C,Bokor A,D'Hooghe TM

    更新日期:2012-11-01 00:00:00

  • Intestinal complications associated with use of the Dexon mesh sling in gynecologic oncology patients.

    abstract::Three cases of postoperative enterocutaneous fistula formation following use of the Dexon mesh sling are reported from two gynecologic oncology services. Two patients had intestinal trauma or bowel resection at the time of mesh placement. Postoperative submesh abscess formation was noted in only one patient. Factors w...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Patsner B,Mann WJ Jr,Chalas E,Orr JW Jr

    更新日期:1990-07-01 00:00:00

  • Illness perceptions and changes in lifestyle following a gynecological cancer diagnosis: A longitudinal analysis.

    abstract:OBJECTIVE:This study explores patterns of lifestyle change and whether more threatening illness perceptions are associated with lifestyle changes post-treatment for smoking, alcohol consumption and Body Mass Index (BMI) among gynecological cancer patients. METHODS:In total, 395 cancer patients (N=221 endometrial; N=17...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,随机对照试验


    authors: van Broekhoven MECL,de Rooij BH,Pijnenborg JMA,Vos MC,Boll D,Kruitwagen RFPM,van de Poll-Franse LV,Ezendam NPM

    更新日期:2017-05-01 00:00:00

  • Retinoblastoma protein expression in ovarian epithelial neoplasms.

    abstract::Progress has been made in identifying the molecular changes that occur in ovarian carcinoma; still our understanding of these changes and their interactions remains incomplete. In the present study the authors examined the expression of retinoblastoma protein, a tumor suppressor protein, in a spectrum of ovarian epith...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Niemann TH,Trgovac TL,McGaughy VR,Lewandowski GS,Copeland LJ

    更新日期:1998-06-01 00:00:00

  • Concurrent chemoradiation in advanced cervical cancer.

    abstract::The pelvis is the predominant site of failure following radical radiotherapy (RT) for locally advanced cervical cancer. We report the results of phase I-II studies on 200 patients with bulky (greater than or equal to 5 cm) carcinoma of the cervix. Patients were treated between 1981 and 1988 on sequential protocols of ...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章,评审


    authors: Thomas G,Dembo A,Fyles A,Gadalla T,Beale F,Bean H,Pringle J,Rawlings G,Bush R,Black B

    更新日期:1990-09-01 00:00:00

  • Risk of malignancy index in the preoperative evaluation of patients with adnexal masses.

    abstract:OBJECTIVE:The aim of this study was to evaluate the use of a Risk of Malignancy Index (RMI) in primary evaluation of patients with adnexal masses. METHODS:The RMI is based on menopausal status, ultrasonographic findings, and serum CA 125 level. A cutoff level of 200 was chosen as the threshold for referral for central...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Andersen ES,Knudsen A,Rix P,Johansen B

    更新日期:2003-07-01 00:00:00

  • Epithelial ovarian cancer and recreational physical activity: A review of the epidemiological literature and implications for exercise prescription.

    abstract:UNLABELLED:Despite the publication of two dozen observational epidemiological studies investigating the association between recreational physical activity (RPA) and epithelial ovarian cancer (EOC) risk and survival over the past two decades, taken collectively, data from retrospective and prospective studies are mixed ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审


    authors: Cannioto RA,Moysich KB

    更新日期:2015-06-01 00:00:00

  • Complete remission of gestational choriocarcinoma with choroidal metastasis treated with systemic chemotherapy alone: case report and review of literature.

    abstract:BACKGROUND:Gestational choriocarcinoma is a malignant tumor that frequently metastasizes to the highly vascularized organs such as the lung, brain, and liver via hematogenous spread. However, this tumor rarely metastasizes to the eye and only a few cases of metastasis to the choroid have been reported. CASE:A 17-year-...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审


    authors: Ino K,Mitsui T,Nomura S,Kikkawa F,Mizutani S

    更新日期:2001-12-01 00:00:00

  • Mucinous adenofibroma of the ovary: case report of the endocrinologic findings.

    abstract::Endocrine and clinicopathologic findings in a 77-year-old woman with ovarian mucinous adenofibroma of borderline malignancy are reported. The preoperative levels of testosterone, androstenedione, estrone, and estradiol in her peripheral blood were 91 ng/ml, 3.78 ng/ml, 82 pg/ml, and 35 pg/ml, respectively, abnormally ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Tohya T,Mizutani H,Katabuchi H,Miyazaki K,Okamura H

    更新日期:1994-08-01 00:00:00

  • Expression and clinical role of long non-coding RNA in high-grade serous carcinoma.

    abstract:OBJECTIVE:To profile long non-coding RNA (lncRNA) expression at the various anatomic sites of high-grades serous carcinoma (HGSC) and in effusion-derived exosomes. METHODS:LncRNA profiling was performed on 60 HGSC specimens, including 10 ovarian tumors, 10 solid metastases and 10 malignant effusions, as well as exosom...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Filippov-Levy N,Cohen-Schussheim H,Tropé CG,Hetland Falkenthal TE,Smith Y,Davidson B,Reich R

    更新日期:2018-03-01 00:00:00

  • Angiographic-guided embolization of metastatic invasive mole.

    abstract:BACKGROUND:The friable and hypervascular nature of a metastatic invasive mole places the patient at risk for significant hemorrhage. Bleeding is the chief cause of morbidity and mortality in patients with a histopathological diagnosis of invasive mole. Bleeding from vaginal metastatic lesions can be controlled by packi...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Method MW,Hirschfield M,Everette HE

    更新日期:1996-06-01 00:00:00

  • Cytoreductive surgery for patients with recurrent epithelial ovarian carcinoma.

    abstract:OBJECTIVE:This study aims to identify favorable preoperative characteristics and examine the impact of secondary cytoreductive surgery on survival for patients with recurrent epithelial ovarian carcinoma. METHODS:Patients who underwent cytoreductive surgery for recurrent epithelial ovarian cancer were identified in ou...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Tebes SJ,Sayer RA,Palmer JM,Tebes CC,Martino MA,Hoffman MS

    更新日期:2007-09-01 00:00:00

  • HPV subtypes and immunological parameters of cervical cancer in Iceland during two time periods, 1958-1960 and 1995-1996.

    abstract:OBJECTIVE:Cervical cancer is a disease caused in part by an infection with an oncogenic subtype of human papillomavirus (HPV). In this study we analysed all cervical cancer samples diagnosed in Iceland during two periods, 1958-1960 and 1995-1996, and asked whether significant changes in viral or immunological parameter...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Mikaelsdottir EK,Benediktsdottir KR,Olafsdottir K,Arnadottir T,Ragnarsson GB,Olafsson K,Sigurdsson K,Kristjansdottir GS,Imsland AK,Ogmundsdottir HM,Rafnar T

    更新日期:2003-04-01 00:00:00

  • Withdrawal from familial ovarian cancer screening for surgery: findings from a psychological evaluation study (PsyFOCS).

    abstract:OBJECTIVE:A prospective psychological evaluation study of familial ovarian cancer screening (PsyFOCS) is underway in partnership with the UK Familial Ovarian Cancer Screening Study (UK FOCSS Phase 2). One of the aims of PsyFOCS is to examine factors associated with withdrawal from the UK FOCSS prior to the onset of 4-m...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Lifford KJ,Fraser L,Rosenthal AN,Rogers MT,Lancastle D,Phelps C,Watson EK,Clements A,Iredale R,Jacobs I,Menon U,Brain KE

    更新日期:2012-01-01 00:00:00

  • Activation of androgen receptor-associated protein 70 (ARA70) mRNA expression in ovarian cancer.

    abstract:OBJECTIVE:Androgens have been implicated in ovarian cancer and androgen receptor expression has been reported in 70-95% of ovarian adenocarcinomas, implying a role in ovarian cancer cell biology. Androgen receptor-associated protein 70 (ARA70) is a reported androgen receptor coactivator that enhances the transactivatio...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Shaw PA,Rittenberg PV,Brown TJ

    更新日期:2001-02-01 00:00:00

  • Diagnosis of endometrial cancer in patients with postmenopausal bleeding by analysis of the lactate dehydrogenase isoenzyme activity profile in uterine fluid.

    abstract:OBJECTIVES:We have previously shown that high activity of lactate dehydrogenase (LD) isoenzymes 4 and 5 in terine aspirates is a marker for endometrial carcinoma. The purpose of this study was to identify an abnormal activity profile of LD2-5 using LD1 as an internal standard, thereby being able to dispense with measur...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Niklasson O,Skude G,Marsal K,Casslén B

    更新日期:2004-05-01 00:00:00

  • Ultrasound guidance for placement of difficult intracavitary implants.

    abstract::Placement of a radiation implant in patients with cervical cancer may be difficult because of distortion of the canal by tumor or fibrosis from previous radiation. The objective of this study was to determine whether ultrasound may be useful in recognizing or preventing complications associated with placement of an in...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Rotmensch J,Waggoner SE,Quiet C

    更新日期:1994-08-01 00:00:00

  • Prognostic factors in patients with bulky stage IB or IIA cervical carcinoma undergoing neoadjuvant chemotherapy and radical hysterectomy.

    abstract::All patients with bulky (> or =4 cm) Stage Ib or IIa cervical carcinoma treated at Chang Gung Memorial Hospital between August 1988 and December 1991 using a strategy of neoadjuvant chemotherapy with cisplatin, vincristine, and bleomycin and radical hysterectomy were reviewed. Fifty-nine evaluable patients received 1 ...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章


    authors: Lai CH,Hsueh S,Chang TC,Tseng CJ,Huang KG,Chou HH,Chen SM,Chang MF,Shum HC

    更新日期:1997-03-01 00:00:00