Abstract：
:Bone scrapers are commonly used to harvest autologous bone in oral and implant surgery. The angle of the cutting blade is a variable that distinguishes bone scrapers. In the present study, the impact of the angle of the cutting blade on the in vitro characteristics of harvested bone was determined. Bone scrapers with blade angles of 15°, 25°, 35°, 45°, and 55° were used to harvest porcine cortical mandibular bone. The number and characteristics of the cells that grew out from the bone chips were examined. The data showed that, independent of the angle of the cutting blade, viable cells were barely detectable in fresh bone grafts. However, cells with a fibroblastic morphology appeared within 1 week in the culture dishes. After 21 days, the number of cells did not differ significantly between the five preparations. Moreover, cells responded to incubation with bone morphogenetic protein 7 (BMP-7) with an increased alkaline phosphatase activity, irrespective of the preparation. The data suggest that bone scrapers with different cutting angles produce bone chips with comparable in vitro characteristics.
journal_name
Int J Oral Maxillofac Surgjournal_title
International journal of oral and maxillofacial surgeryauthors
Kuchler U,Schmid C,Buser D,Gruber Rdoi
10.1016/j.ijom.2013.11.014subject
Has Abstractpub_date
2014-07-01 00:00:00pages
879-82issue
7eissn
0901-5027issn
1399-0020pii
S0901-5027(13)01194-6journal_volume
43pub_type
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