Abstract：
BACKGROUND:Group B streptococcal infections are the leading cause of sepsis and meningitis in newborns. A rapid and reliable method for the detection of this pathogen at the time of delivery is needed for the early treatment of neonates. Isothermal amplification techniques such as recombinase polymerase amplification have advantages relative to PCR in terms of the speed of reaction and simplicity. METHODS:We studied the clinical performance of recombinase polymerase amplification for the screening of group B streptococci in vaginal/anal samples from 50 pregnant women. We also compared the limit of detection and the analytical specificity of this isothermal assay to real-time PCR (RT-PCR). RESULTS:Compared to RT-PCR, the recombinase polymerase amplification assay showed a clinical sensitivity of 96% and a clinical specificity of 100%. The limit of detection was 98 genome copies and the analytical specificity was 100% for a panel of 15 bacterial and/or fungal strains naturally found in the vaginal/anal flora. Time-to-result for the recombinase polymerase amplification assay was <20 min compared to 45 min for the RT-PCR assay; a positive sample could be detected as early as 8 min. CONCLUSIONS:We demonstrate the potential of isothermal recombinase polymerase amplification assay as a clinically useful molecular diagnostic tool that is simple and faster than PCR/RT-PCR. Recombinase polymerase amplification offers great potential for nucleic acid-based diagnostics at the point of care.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Daher RK,Stewart G,Boissinot M,Bergeron MGdoi
10.1373/clinchem.2013.213504subject
Has Abstractpub_date
2014-04-01 00:00:00pages
660-6issue
4eissn
0009-9147issn
1530-8561pii
clinchem.2013.213504journal_volume
60pub_type
杂志文章abstract：:In photometry, where both the product formed and one or several reactants absorb light at the same wavelength, the absorbance of the "blank" of the sample at the end of the reaction may be less than that measured at the beginning of the reaction, because of consumption of reactant(s). The blank correction factor for t...
journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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doi：
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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doi：
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更新日期：1995-09-01 00:00:00
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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doi：
更新日期：1987-10-01 00:00:00
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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doi：
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journal_title：Clinical chemistry
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doi：
更新日期：1997-07-01 00:00:00
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journal_title：Clinical chemistry
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