Abstract：
:A method was developed for the assay of benzoic acid, 2-chloro-5-[[(1-methylethoxyl)thioxomethyl]amino]-,1-meth yle thyl ester (NSC 629243) in hamster, mouse, human and, to a limited extent, dog plasma. Protein in 0.5 ml of plasma was precipitated with four volumes of methanol and the supernatant was analysed for NSC 629243 by LC. Liquid chromatography was carried out on a reversed-phase Nova-Pak C18 column, with a mobile phase of 60% acetonitrile in water at 1 ml min-1, and the compound was quantified with a UV detector set at 283 nm. Two standard curves of NSC 629243 were needed to cover a concentration range of 0.05-100 micrograms ml-1. All standard curves had correlation coefficients > 0.999. In practice, the minimum quantifiable concentration was approximately 0.05 microgram ml-1 in 0.5 ml of plasma. NSC 629243 appeared to have good stability at 37 degrees C in hamster, human and dog plasma at concentrations of 1 and 50 micrograms ml-1 (at least 80% remained in plasma after a 4 h incubation). Breakdown occurred in mouse plasma after 1 h at 37 degrees C, with extensive breakdown occurring after 24 h. NSC 629243 was extensively bound to plasma proteins of Syrian hamsters and humans. The extent of binding ranged from a minimum of 88.6% to a maximum of 99.9% over a concentration range of ca 1-100 micrograms ml-1.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Nomeir AA,McComish MF,Mongan AL,Covey JM,Ferrala NFdoi
10.1016/0731-7085(94)80037-5subject
Has Abstractpub_date
1994-05-01 00:00:00pages
693-8issue
5eissn
0731-7085issn
1873-264Xpii
0731-7085(94)80037-5journal_volume
12pub_type
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