Abstract：
:A reversed-phase high-performance liquid chromatography (RP-HPLC) method with UV-detection at room temperature has been developed for the analysis of insulin and its main degradation product, A21-desamido insulin. Octadecylsilica was used as stationary phase and a mixture of water and acetonitrile containing tetrametylammonium hydroxide as eluent. The method produced linear response over the concentration range of 10-100 microg/ml, with an average accuracy of 97.35+/-1.36% as well as average intra- and inter-day variations of 1.29 and 5.24%, respectively. The limits of detection and quantitation of the method were 0.25 and 0.75 microg/ml, respectively. Considering the analysis specifications, the system is suitable for direct analysis of routine formulations and stability studies. By this method human insulin can be separated from its principal degradation product, A21-desamido insulin. Also there is no need for any particular requirement and it is easily available in most of laboratories.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Moslemi P,Najafabadi AR,Tajerzadeh Hdoi
10.1016/s0731-7085(03)00336-4subject
Has Abstractpub_date
2003-09-15 00:00:00pages
45-51issue
1eissn
0731-7085issn
1873-264Xpii
S0731708503003364journal_volume
33pub_type
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章,随机对照试验
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更新日期：2018-08-05 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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