Abstract:
:In this work the interaction of DNA and the flavonoid-transition metal complex (Cu(II)-naringin complex) is characterized. The interaction was evaluated by using electrochemical ssDNA- and dsDNA-based biosensors and the results were supported by UV, CD and (1)H NMR data. In the electrochemical method, changes in the oxidation peak of the guanine and adenine bases obtained by square wave voltammetry (SWV) showed evidence of the interaction. The variations of the spectroscopic characteristics of DNA and Cu(II)-naringin complex in aqueous medium demonstrated that the predominant interaction mode may be by intercalation. Cu(II)-naringin complex interacts to dsDNA probably via N(7) of guanine site.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Mello LD,Pereira RM,Sawaya AC,Eberlin MN,Kubota LTdoi
10.1016/j.jpba.2007.08.007subject
Has Abstractpub_date
2007-12-21 00:00:00pages
706-13issue
5eissn
0731-7085issn
1873-264Xpii
S0731-7085(07)00467-0journal_volume
45pub_type
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