Abstract：
:A fast liquid chromatography-tandem mass spectrometry method using polarity switching and timed selected reaction monitoring has been developed and validated to quantify eight antiepileptic drugs and an active drug metabolite in human plasma within a single analytical assay. The antiepileptic drugs include levetiracetam, lamotrigine, zonisamide, topiramate, carbamazepine, phenytoin, divalproex sodium, oxcarbazepine, and the oxcarbazepine active metabolite 10,11-dihydro-10-hydroxycarbamazepine. The method was validated over concentration ranges specifically for each compound, with a lower limit of quantification of 5-50 ng/mL. The method is accurate and precise, with intra-day and inter-day accuracy ranging from 93.7 to 104.7% and 94.1 to 107.1%, respectively, and intra-day and inter-day coefficient of variation (CV) ranging of 0.8-4.4% and 1.5-7.1%, respectively. Simple two-step protein precipitation and dilution sample preparation, high sensitivity, and high throughput make this method suitable for multi-drug monitoring for epileptic patients.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Liu T,Kotha RR,Jones JW,Polli JE,Kane MAdoi
10.1016/j.jpba.2019.112816subject
Has Abstractpub_date
2019-11-30 00:00:00pages
112816eissn
0731-7085issn
1873-264Xpii
S0731-7085(19)31173-2journal_volume
176pub_type
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