Structure and biotechnological applications of odorant-binding proteins.

abstract：:Escherichia coli normally cannot utilize the β-glucoside sugar cellobiose as a carbon and energy source unless a stringent selection pressure for survival is present. The cellobiose-utilization phenotype can be conferred by mutations in the two cryptic operons, chb and asc. In this study, the cellobiose-utilization ph...

journal_title：Applied microbiology and biotechnology

authors： Vinuselvi P,Lee SK

更新日期：2011-10-01 00:00:00

abstract：:Insertion specificity of mobile genetic elements is a rather complex aspect of DNA transposition, which, despite much progress towards its elucidation, still remains incompletely understood. We report here the results of a meta-analysis of IS2 target sites from genomic, phage, and plasmid DNA and find that newly acqui...

journal_title：Applied microbiology and biotechnology

authors： Gonçalves GA,Oliveira PH,Gomes AG,Prather KL,Lewis LA,Prazeres DM,Monteiro GA

更新日期：2014-08-01 00:00:00

abstract：:The removal of hydrophobic volatile organic compounds (VOCs) still remains the main restriction in the biological treatment of odorous emissions due to mass transfer limitations. The addition of a non-aqueous phase to conventional biotrickling filters (BTF) may overcome this limitation by enhancing VOCs transport from...

journal_title：Applied microbiology and biotechnology

authors： Lebrero R,Rodríguez E,Pérez R,García-Encina PA,Muñoz R

更新日期：2013-05-01 00:00:00

abstract：:Polygalacturonases (PG) have evolved in the past years from a pectinase "simply" being used for food processing to an important parameter in plant-fungal interaction. PG-inhibiting proteins (PGIP) that are synthesised in plants as a specific response to PGs of pathogenic fungi, have become a focus as a possible target...

journal_title：Applied microbiology and biotechnology

authors： Lang C,Dörnenburg H

更新日期：2000-04-01 00:00:00

abstract：:A gratuitous strain was developed by disrupting the GAL1 gene (galactokinase) of recombinant Saccharomyces cerevisiae harboring the antithrombotic hirudin gene in the chromosome under the control of the GAL10 promoter. A series of glucose-limited fed-batch cultures were carried out to examine the effects of glucose su...

journal_title：Applied microbiology and biotechnology

authors： Kim MD,Lee TH,Lim HK,Seo JH

更新日期：2004-08-01 00:00:00

abstract：:Lactobacillus plantarum MA2 was isolated from traditional Chinese Tibet kefir grains, which possess several excellent properties and functions. We previously demonstrated the antioxidant activities of this bacterium in vitro. However, the maintenance and survival of L. plantarum MA2 inside the murine intestinal tract,...

journal_title：Applied microbiology and biotechnology

authors： Tang W,Xing Z,Hu W,Li C,Wang J,Wang Y

更新日期：2016-08-01 00:00:00

abstract：:We used biodegradable plastics as fermentation substrates for the filamentous fungus Aspergillus oryzae. This fungus could grow under culture conditions that contained emulsified poly-(butylene succinate) (PBS) and emulsified poly-(butylene succinate-co-adipate) (PBSA) as the sole carbon source, and could digest PBS a...

journal_title：Applied microbiology and biotechnology

authors： Maeda H,Yamagata Y,Abe K,Hasegawa F,Machida M,Ishioka R,Gomi K,Nakajima T

更新日期：2005-06-01 00:00:00

abstract：:The utilization of monochlorobenzoate isomers (2-, 3- and 4-chlorobenzoate) by anaerobic microbial consortia in River Nile sediments was systematically evaluated under denitrifying, Fe-reducing, sulfidogenic and methanogenic conditions. Loss of all three chlorobenzoates was noted in denitrifying cultures; furthermore,...

journal_title：Applied microbiology and biotechnology

authors： Kazumi J,Häggblom MM,Young LY

更新日期：1995-10-01 00:00:00

abstract：:Process intensification and integration is crucial regarding an ever increasing pressure on manufacturing costs and capacities in biologics manufacturing. For virus production in perfusion mode, membrane-based alternating tangential flow filtration (ATF) and acoustic settler are the commonly described cell retention t...

journal_title：Applied microbiology and biotechnology

authors： Gränicher G,Coronel J,Trampler F,Jordan I,Genzel Y,Reichl U

更新日期：2020-06-01 00:00:00

abstract：:Due to variations in the production levels, a full-scale sequencing batch reactor (SBR) for post-treatment of tannery wastewater was exposed to low and high ammonia load periods. In order to study how these changes affected the N-removal capacity, the microbiology of the reactor was studied by a diverse set of techniq...

journal_title：Applied microbiology and biotechnology

authors： Cabezas A,Draper P,Etchebehere C

更新日期：2009-10-01 00:00:00

abstract：:Aspartic proteases are a relatively small group of proteolytic enzymes that are active in acidic environments and are found across all forms of life. Certain microorganisms secrete such proteases as virulence agents and/or in order to break down proteins thereby liberating assimilable sources of nitrogen. Some of the ...

journal_title：Applied microbiology and biotechnology

authors： Theron LW,Divol B

更新日期：2014-11-01 00:00:00

abstract：:PCR-based analysis of Bacteroidales 16S rRNA genes has emerged as a promising tool to identify sources of fecal water pollution. In this study, three TaqMan real-time PCR assays (BacGeneral, BacHuman, and BacBovine) were developed and evaluated for their ability to quantitatively detect general (total), human-specific...

journal_title：Applied microbiology and biotechnology

authors： Lee DY,Weir SC,Lee H,Trevors JT

更新日期：2010-12-01 00:00:00

abstract：:A beta-lactamase-free penicillin amidase from Alcaligenes sp. active against various beta-lactams was purified to homogeneity. The enzyme can hydrolyze penicillin G to 6-amino penicillanic acid (6-APA) and furnish penicillin G from 6-APA and phenyl acetic acid by condensation. The penicillin amidase is a heterodimer o...

journal_title：Applied microbiology and biotechnology

authors： Das S,Gayen JR,Pal A,Ghosh K,Rosazza JP,Samanta TB

更新日期：2004-08-01 00:00:00

abstract：:Optimization of yields and productivities in reductive whole-cell biotransformations is an important issue for the industrial application of such processes. In a recent study with Escherichia coli, we analyzed the reduction of the prochiral β-ketoester methyl acetoacetate by an R-specific alcohol dehydrogenase (ADH) t...

journal_title：Applied microbiology and biotechnology

authors： Siedler S,Bringer S,Blank LM,Bott M

更新日期：2012-02-01 00:00:00

abstract：:A moderately halophilic alkalitolerant Bacillus sp. Strain TSCVKK, with an ability to produce extracellular halophilic, alkalitolerant, surfactant, and detergent-stable alpha-amylase was isolated from soil samples obtained from a salt-manufacturing industry in Chennai. The culture conditions for higher amylase product...

journal_title：Applied microbiology and biotechnology

authors： Kiran KK,Chandra TS

更新日期：2008-01-01 00:00:00

abstract：:Thermochemical processing of biomass by fast pyrolysis provides a nonenzymatic route for depolymerization of biomass into sugars that can be used for the biological production of fuels and chemicals. Fermentative utilization of this bio-oil faces two formidable challenges. First is the fact that most bio-oil-associate...

journal_title：Applied microbiology and biotechnology

authors： Jarboe LR,Wen Z,Choi D,Brown RC

更新日期：2011-09-01 00:00:00

abstract：:Determining spatiotemporal gene expression and analyzing knockout mutant phenotypes have become powerful tools in elucidating the function of genes; however, genetic approaches for simultaneously inactivating a gene and monitoring its expression have not been reported in the literature. In this study, we designed a du...

journal_title：Applied microbiology and biotechnology

authors： Chen K,Xu X,Gu L,Hildreth M,Zhou R

更新日期：2015-02-01 00:00:00

abstract：:Acetobacter xylinum BPR2001 produces water-insoluble bacterial cellulose (BC). Using a pH sensor for the accurate control of pH, which is one of the most critical factors for efficient BC production, is difficult especially in a baffled shake-flask and an airlift reactor. The buffering capacity of corn steep liquor (C...

journal_title：Applied microbiology and biotechnology

authors： Noro N,Sugano Y,Shoda M

更新日期：2004-04-01 00:00:00

abstract：:Biocides play an important role in the preservation of white mineral dispersions (WMD). Due to the occurrence of biocide-resistant bacteria and technical limitations in the use of biocides, new preservation strategies are required-like the enhancement of biocides by non-biocidal compounds. The aim of this study was to...

journal_title：Applied microbiology and biotechnology

authors： Di Maiuta N,Schwarzentruber P,Dow CS

更新日期：2011-01-01 00:00:00

abstract：:Previous investigations have reported that bacterial suspension cultures grow to higher stationary concentrations in space flight than on Earth; however, none of these investigations included extensive ground controls under varied inertial conditions. This study includes extensive controls and cell-growth data taken a...

journal_title：Applied microbiology and biotechnology

authors： Kacena MA,Merrell GA,Manfredi B,Smith EE,Klaus DM,Todd P

更新日期：1999-02-01 00:00:00

abstract：:Escherichia coli and Lactobacillus plantarum were subjected to final water potentials of -5.6 MPa and -11.5 MPa with three solutes: glycerol, sorbitol and NaCl. The water potential decrease was realized either rapidly (osmotic shock) or slowly (20 min) and a difference in cell viability between these conditions was on...

journal_title：Applied microbiology and biotechnology

authors： Poirier I,Maréchal PA,Evrard C,Gervais P

更新日期：1998-12-01 00:00:00

abstract：:Increasing evidence has shown that fecal microbiota transplantation (FMT) could be a promising treatment option for Crohn's disease (CD). However, the frequency of FMT for CD treatment remains unclear. This study aimed to evaluate the optimal timing for administering the second course of FMT to maintain the long-term ...

journal_title：Applied microbiology and biotechnology

authors： Li P,Zhang T,Xiao Y,Tian L,Cui B,Ji G,Liu YY,Zhang F

更新日期：2019-01-01 00:00:00

abstract：:A novel fluoro-containing jasmonate derivative was chemically synthesized and evaluated as a potential elicitor with respect to the induction of plant defense responses and the biosynthesis of plant secondary metabolites. A bioactive taxuyunnanine C (Tc)-producing cell line of Taxus chinensis was taken as a model plan...

journal_title：Applied microbiology and biotechnology

authors： Qian ZG,Zhao ZJ,Xu Y,Qian X,Zhong JJ

更新日期：2005-07-01 00:00:00

abstract：:A cDNA of a structural gene encoding pyranose 2-oxidase (P2O) from Trametes ochracea strain MB49 was cloned into Escherichia coli strain BL21(DE3) on a multicopy plasmid under the control of the trc promoter. Synthesis of P2O was studied in batch cultures in LB or M9-based mineral medium at 28 degrees C. While there w...

journal_title：Applied microbiology and biotechnology

authors： Vecerek B,Maresová H,Kocanová M,Kyslík P

更新日期：2004-05-01 00:00:00

abstract：:Cultivation of Aspergillus terreus ATCC 20542 in a stirred tank bioreactor was performed to induce the biosynthesis of secondary metabolites and provide the bioprocess-related insights into the metabolic capabilities of the investigated strain. The activation of biosynthetic routes was attempted by the diversification...

journal_title：Applied microbiology and biotechnology

authors： Boruta T,Bizukojc M

更新日期：2016-04-01 00:00:00

abstract：:N-acetyl glutamate kinase (NAGK) is a key enzyme in the synthesis of L-arginine that is inhibited by its end product L-arginine in Corynebacterium glutamicum (C. glutamicum). In this study, the potential binding sites of arginine and the residues essential for its inhibition were identified by homology modeling, inhib...

journal_title：Applied microbiology and biotechnology

authors： Huang Y,Zhang H,Tian H,Li C,Han S,Lin Y,Zheng S

更新日期：2015-09-01 00:00:00

abstract：:For the fermentative production of plant-specific flavanones (naringenin, pinocembrin) by Escherichia coli, a plasmid was constructed which carried an artificial biosynthetic gene cluster, including PAL encoding a phenylalanine ammonia-lyase from a yeast, ScCCL encoding a cinnamate/coumarate:CoA ligase from the actino...

journal_title：Applied microbiology and biotechnology

authors： Miyahisa I,Kaneko M,Funa N,Kawasaki H,Kojima H,Ohnishi Y,Horinouchi S

更新日期：2005-09-01 00:00:00

abstract：:The human antimicrobial peptide LL-37 is a cationic peptide with antimicrobial activity against both Gram-positive and Gram-negative microorganisms. This work describes the development of an expression system based on Escherichia coli capable of high production of the recombinant LL-37. The fusion protein Trx-LL-37 wa...

journal_title：Applied microbiology and biotechnology

authors： Krahulec J,Hyrsová M,Pepeliaev S,Jílková J,Cerný Z,Machálková J

更新日期：2010-09-01 00:00:00

abstract：:Robustness of fermenting strains to lignocellulose derived inhibitors is critical for efficient biofuel and biochemical productions. In this study, the industrial fermenting strain Corynebacterium glutamicum S9114 was evolved for improved inhibitor tolerance using long-term adaptive evolution by continuously transferr...

journal_title：Applied microbiology and biotechnology

authors： Wang X,Khushk I,Xiao Y,Gao Q,Bao J

更新日期：2018-01-01 00:00:00

abstract：:Actinomycetes are the most important producers of secondary metabolites for medical, agricultural and industrial applications. Efficient engineering of bacterial genomes to improve their biosynthetic capabilities largely depends on the available arsenal of tools and vectors. One of the most widely used vector systems ...

journal_title：Applied microbiology and biotechnology

authors： Muth G

更新日期：2018-11-01 00:00:00