Abstract:
:Diagnosis of allergic conditions is based on skin prick- and/or intradermal tests and on serology. Both methods make use of allergenic preparations and the results mainly depend on the quality of the substances applied and on the reactivity of the subjects. Recombinant allergens can be produced as highly pure proteins. They may contribute to a significant improvement of the diagnosis of allergic diseases provided that the allergens became available in a pure, fully antigenic form suitable for routine assessments. We have studied the diagnostic properties of recombinant Aspergillus fumigatus allergen I (rAsp f I/a), a major allergen recognised by about 60% of the A. fumigatus allergic individuals, to test the feasibility of using recombinant allergens for automated serology in the Pharmacia CAP System, a new solid-phase immunoassay. ImmunoCAPs carrying immobilised rAsp f I/a were produced and evaluated using sera from well characterised patients with allergic bronchopulmonary aspergillosis (ABPA), allergic asthmatics with or without A. fumigatus sensitisation, and healthy control persons. The clinical parameters used for the diagnosis of allergy to A. fumigatus were RAST and skin test reactivity to commercial A. fumigatus extracts. IgE antibodies recognising rAsp f I/a were determined by a direct antigen-specific ELISA and with the newly developed rAsp f I/a ImmunoCAPs. Quantitative results from the two rAsp f I/a detection systems correlated closely, indicating that this recombinant allergen can be incorporated in the Pharmacia CAP System with unaltered antigenic activity. The behaviour of a panel of recombinant A. fumigatus allergens in the Pharmacia CAP System will be evaluated in the near future to assess the potential of recombinant allergens for the automated diagnosis of allergic conditions.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Crameri R,Lidholm J,Menz G,Grönlund H,Blaser Kdoi
10.1007/978-1-4615-5855-2_15subject
Has Abstractpub_date
1996-01-01 00:00:00pages
111-6eissn
0065-2598issn
2214-8019journal_volume
409pub_type
杂志文章abstract::The purpose of the paper was to investigate the usefulness of posturographic analysis in patients with rheumatoid arthritis (RA) and osteoarthritis (OA). RA is a chronic inflammatory disorder responsible for destruction of active and passive components of joints. It is the most common autoimmune disease, and the secon...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/5584_2014_29
更新日期:2015-01-01 00:00:00
abstract::The first systematic multi-center study of the clinical use of EPR oximetry has begun, with funding as a PPG from the NCI. Using particulate oxygen sensitive EPR, materials in three complementary forms (India Ink, "OxyChips", and implantable resonators) the clinical value of the technique will be evaluated. The aims i...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,多中心研究
doi:10.1007/978-3-319-38810-6_13
更新日期:2016-01-01 00:00:00
abstract::As we enter the 2000's it is clear that cytokine blockade is an effective therapeutic strategy for rheumatoid arthritis. In this brief review, we will review the rationale for anti TNFalpha therapy, the current status of therapy and focus on the regulation of TNFalpha production in rheumatoid synovium. New approaches ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4615-1243-1_13
更新日期:2001-01-01 00:00:00
abstract::In the visible/NIR (600-900 nm) three different redox centres are potentially detectable in vivo in mitochondrial cytochrome c oxidase: haem a (605nm), the binuclear haem a3/CuB centre (655 nm) and CuA (830 nm). In this paper we report changes in the steady state reduction of these centres following increases in the r...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-0-387-85998-9_19
更新日期:2009-01-01 00:00:00
abstract::Glucocorticoids affect the developing and mature cardiovascular system in profound and, at times, contradictory ways. The glucocorticoid receptor is ubiquitous in most cell types and conserved across species, highlighting its importance in development and homeostasis. Despite the fact that the glucocorticoid receptor ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4939-2895-8_13
更新日期:2015-01-01 00:00:00
abstract::Use of urea as a nonprotein supplement in the feeding of beef cattle and other ruminant animals is a technique of practical application. Urea has also been used in human feeding studies. Its early employment was that of an added source of amino nitrogen in studies designed to determine minimum requirements of the esse...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-3366-1_7
更新日期:1978-01-01 00:00:00
abstract::Tropomyosins constitute a family of highly related actin-binding proteins found in the animal kingdom from yeast to human. In vertebrates, they are encoded by a multigene family where each member can produce several isoforms through alternative splicing and for some of them with alternate promoters. Tropomyosin isofor...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-0-387-85766-4_2
更新日期:2008-01-01 00:00:00
abstract::Collective cell migration plays key roles in various physiological and pathological processes in multicellular organisms, including embryonic development, wound healing, and formation of cancer metastases. Such collective migration involves complex crosstalk among cells and their environment at both biochemical and me...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-030-17593-1_1
更新日期:2019-01-01 00:00:00
abstract::Mouse embryonic stem cells (mESCs) release into the medium in which they are cultured heterogeneous populations of microvesicles (mESMVs), important components of cell-cell communication, that transfer their contents not only to other stem cells but also to cells of other origins. The purpose of these studies was to d...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-17121-0_75
更新日期:2016-01-01 00:00:00
abstract::A20 is most characteristically described in terms relating to inflammation and inflammatory pathologies. The emerging understanding of inflammation in the etiology of diabetes mellitus lays the framework for considering a central role for A20 in this disease process. Diabetes mellitus is considered a major health issu...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4939-0398-6_9
更新日期:2014-01-01 00:00:00
abstract::Two different neural implementations of support vector machines are described and applied to one-shot trainable pattern recognition. The first model is based on oscillating associative memory and is mapped to the olfactory system. The second model is founded on competitive queuing memory originally employed for genera...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4614-0164-3_16
更新日期:2011-01-01 00:00:00
abstract::Obesity and its co-morbidities as metabolic syndrome, type 2 diabetes mellitus and cardiovascular diseases are major health problems worldwide. Several reports indicated that nutrient excess and metabolic syndrome are linked with altered immune response. Indeed, metabolic syndrome is characterized by insulin resistanc...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-07320-0_13
更新日期:2014-01-01 00:00:00
abstract::The diffusion coefficients of cell membranes of pneumocytes plus endothelial cells, cytosol plus blood plasma, erythrocyte membranes, and hemoglobin solution in erythrocytes were estimated from the fluorometrically measured membrane viscosity. The time course of oxygen partial pressure distribution was numerically cal...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-9510-6_7
更新日期:1988-01-01 00:00:00
abstract::Laminins are major components of all basement membranes surrounding nerve or vascular tissues. In particular laminin-111, the prototype of the family, facilitates a large spectrum of fundamental cellular responses in all eukaryotic cells. Laminin-111 is a biomaterial frequently used in research, however it is primaril...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-981-13-0947-2_1
更新日期:2018-01-01 00:00:00
abstract::Stem cells serve as persistent reservoirs for replenishment of rapidly renewing tissues, frequently also ensuring that the correct tissue morphology is maintained. This process is inherently stochastic due to the small number and stochastic division patterns within the stem cell compartments, as well as the essentiall...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4419-7210-1_11
更新日期:2012-01-01 00:00:00
abstract::Tracking of tissue oxygenation around chronic foot wounds may help direct therapy decisions in patients with peripheral artery disease (PAD). Novel sensing technology to enable such monitoring was tested over 9 months in a Sinclair mini-pig model. No adverse events were observed over the entire study period. Systemic ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-91287-5_56
更新日期:2018-01-01 00:00:00
abstract::Acute disseminated encephalomyelitis (ADEM) is a disorder of the central nervous system (CNS) characterized by an acute event, typically with encephalopathy, in which diffuse CNS involvement occurs. It may follow an infectious event and occurs more commonly in young children. Pulse steroid treatment is frequently used...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4614-0653-2_1
更新日期:2012-01-01 00:00:00
abstract::Cytochrome P-450 has been purified from liver microsomes of phenobarbital-induced rabbits in the presence of ionic and nonionic detergents to concentrations over 17 nmoles per mg of protein. The purified cytochrome P-450 LM gives a single major band on SDS-polyacrylamide gel electrophoresis representing about 90 per c...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-9026-2_2
更新日期:1975-01-01 00:00:00
abstract::Escherichia coli is one of the most-studied species of bacteria due to its frequent incidence in diverse environments and hosts, as well as its use as a tool in molecular biology. Most E. coli strains are commensal, in that they colonize the host without causing disease; however, some strains of E. coli are pathogens ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-20215-0_9
更新日期:2016-01-01 00:00:00
abstract::Born 200 years ago, Darwin's revolutionary ideas were derived largely from his observations on life forms that evolved relatively recently, including various flowering plants, worms, birds and domesticated animals. Yet, life appeared on planet earth close to 4 billion years ago in the form of unicellular organisms col...
journal_title:Advances in experimental medicine and biology
pub_type: 历史文章,杂志文章
doi:10.1007/978-1-4419-7185-2_6
更新日期:2011-01-01 00:00:00
abstract::Thermomechanical Analysis (TMA), using the parallel-plate-rheometer mode of the DuPont 943 TMA, was used to evaluate the softening properties of frozen aqueous carbohydrate model systems. A sample (of fixed dimension) of frozen solution was placed between the parallel plates and cooled to -80 degrees C. The sample was...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4899-0664-9_11
更新日期:1991-01-01 00:00:00
abstract::The aim of this work was comparison of two algorithms of perfusion computed tomography (PCT) data analysis for evaluation of cerebral microcirculation in the perifocal zone of chronic subdural hematoma (CSDH). Twenty patients with CSDH after polytrauma were included in the study. The same PCT data were assessed quanti...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-38810-6_53
更新日期:2016-01-01 00:00:00
abstract::Drosophila melanogaster is since decades the most important invertebrate model. With the publishing of the genome sequence, Drosophila also became a pioneer in (neuro)peptide research. Neuropeptides represent a major group of signaling molecules that outnumber all other types of neurotransmitters/modulators and hormon...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4419-6902-6_10
更新日期:2010-01-01 00:00:00
abstract::Within the cochlea, broadband sounds like speech and music are filtered into a series of narrowband signals, each of which can be considered as a relatively slowly varying envelope (ENV) imposed on a rapidly oscillating carrier (the temporal fine structure, TFS). Information about ENV and TFS is conveyed in the timing...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-25474-6_1
更新日期:2016-01-01 00:00:00
abstract::1) Although virulence of enteropathogenic E. coli in the pig is essentially associated with the K88 adhesion determinant, protection of the neonate and weanling is not necessarily dependent upon antibodies to this surface antigen. 2) Modification of the surface antigenic characteristics of enteropathogens occurs in th...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-3369-2_16
更新日期:1978-01-01 00:00:00
abstract::C. elegans germline stem cells exist within a stem cell pool that is maintained by a single-celled mesenchymal niche and Notch signaling. Downstream of Notch signaling, a regulatory network governs stem cells and differentiation. Central to that network is the FBF RNA-binding protein, a member of the widely conserved ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-94-007-6621-1_3
更新日期:2013-01-01 00:00:00
abstract::Chemokines are a family of small structurally related molecules that were recognized originally for their ability to regulate cell trafficking in inflammation. We have found that a chemokine, CXC chemokine ligand 12/stromal cell-derived factor/pre-B-cell growth stimulating factor (CXCL12/ SDF-1/PBSF) and its physiolog...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-0-387-72009-8_9
更新日期:2007-01-01 00:00:00
abstract::T-type Ca(2+) channels are a distinct family of low voltage-activated Ca(2+) channels which serve many roles in different tissues. Several studies have implicated them, for example, in the adaptive responses to chronic hypoxia in the cardiovascular and endocrine systems. Hydrogen sulfide (H(2)S) was more recently disc...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-18440-1_40
更新日期:2015-01-01 00:00:00
abstract::Capillary-to-fiber perimeter ratio is a morphometric estimate of muscle capillarity in transverse sections which accounts for the three-dimensional arrangement of the capillary network. We compared different methods for estimating capillary-to-fiber perimeter ratio in muscles with large differences in fiber size and c...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-2468-7_87
更新日期:1994-01-01 00:00:00
abstract::Neisseria meningitidis lipooligosaccharides (LOSs) are classified into 12 immunotypes. Most LOSs are heterogeneous in having a few components by SDS-PAGE analysis that differ antigenically and chemically. We have utilized a monoclonal antibody that recognizes lacto-N-neotetraose (LNnT) and the lectin, Maackia amurensi...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4615-1267-7_35
更新日期:2001-01-01 00:00:00