Abstract:
:Rapid and accurate diagnosis of cytomegalovirus (CMV) infection is imperative with the advent of effective antiviral therapy (gangiclovir, foscarnet, CMV hyperimmune globin). Applications of conventional cell culture (CC), shell vial assay (SV), serological testing, antigenemia assay (AG) as well as molecular methods [polymerase chain reaction (PCR), branch DNA (b-DNA) and hybrid capture (HC)] to various patient populations and specimen types are discussed. A three year study of 670 specimens [354 urines, 205 peripheral blood leukocytes (PBLs), 56 upper respiratory and 55 tissues] compared CMV CC and SV isolation rates. Of the total, 124 (18.5%) were positive by either or both techniques. For each specimen type the number of positives detected by SV was greater than CC (urine 28 vs 15, PBLs, 12 vs 2). However, of 124 positives, 21 were solely CC positive. A comparison of SV to AG in 230 PBLs yielded a sensitivity of 100% and specificity of 68.3%. The low specificity when compared to SV may be due to the increased sensitivity of AG. Fifty-nine PBLs were examined for differing immunostaining techniques [immunoperoxidase (IP) vs Immunofluorescence (IF)]. IF stained PBLs showed an increased number of positive cells per preparation and greater stain intensity for ease of interpretation.
journal_name
Immunol Investjournal_title
Immunological investigationsauthors
Myers JB,Amsterdam Ddoi
10.3109/08820139709022694subject
Has Abstractpub_date
1997-04-01 00:00:00pages
383-94issue
3eissn
0882-0139issn
1532-4311journal_volume
26pub_type
杂志文章abstract::The antiviral activity of cell-associated, non-elutable recombinant human gamma interferon (rHuIFN-gamma) was neutralized by antibody. The neutralization of cell-associated rHuIFN-gamma was maximal through 2 h (60-100%) and declined through 8 h (20-40%). Concomitantly, the antiviral activity of cell-associated [Met-Gl...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139009052977
更新日期:1990-10-01 00:00:00
abstract::Background: Immunosuppressive agents are necessary to enhance allograft tolerance after transplantation and the treatment of autoimmune disorders. Regulatory T cells (Tregs) play a pivotal role in improving allograft tolerance and determining the fate of transplanted organs. Therefore, the aim of this study was to inv...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1571506
更新日期:2019-07-01 00:00:00
abstract::A disintegrin and metalloproteinases (ADAMs) are believed to be involved in the pathogenesis of many fibrosis-related diseases. However, little is known regarding the significance of ADAM17 as a biomarker for interstitial lung disease (ILD). In this study, by using the RT-PCR, western blotting and ELISA, we detected t...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1660367
更新日期:2020-02-01 00:00:00
abstract::Myeloid derived suppressor cells (MDSCs) are a heterogeneous population of cells that inhibit anti-tumor immunity through a variety of mechanisms. Malignant gliomas are heavily infiltrated by myeloid cells, some of which appear to share biological functions of MDSCs. Our data with mouse de novo gliomas indicate critic...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139.2012.689591
更新日期:2012-01-01 00:00:00
abstract::The transferrin receptor (TR), the HLA DR antigen (DR), and the antigen binding OKT10 (T10) are present on activated lymphocyte populations. The authors have studied their expression and that of antigens defined by eight commercial monoclonal antibodies on peripheral blood lymphocytes of 50 healthy hospital workers ag...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709087089
更新日期:1987-06-01 00:00:00
abstract:BACKGROUND:Cancer immunotherapies have achieved much success and have become the forefront treatment of cancers previously associated with poor prognosis. However, a major challenge in cancer immunotherapies remains the heterogeneity of the immunoregulatory capacities of cancers, and not all patients of a given cancer ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2020.1778024
更新日期:2020-06-23 00:00:00
abstract::Antisera to the chemically synthesized trisaccharide, Gal beta 1-3(GlcNAc beta 1-6)GalNAc, and disaccharide, GlcNAc beta 1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically s...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139109082633
更新日期:1991-08-01 00:00:00
abstract::Although the T cell costimulatory molecules CD2 and CD28 are enriched within the immunological synapse (IS), it has been suggested that costimulatory molecules need not be localized to the contact site between a T cell and an antigen-presenting cell (APC) in order to costimulate T cell activation. To determine whether...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130701555035
更新日期:2008-01-01 00:00:00
abstract::The lectin from Amaranthus leucocarpus (ALL) is specific for N-acetyl-D-galactosamine and inhibits phagocytosis of Leishmania mexicana promastigotes in Balb/c mice peritoneal macrophages by 38%. The lipophosphoglycan (LPG) purified from L. mexicana inhibits penetration of promastigotes into peritoneal macrophages by 3...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::Uric acid can be generated in the gastrointestinal (GI) tract from the breakdown of nucleotides ingested in the diet or from purines released from host cells as a result of pathogen-induced cell damage. Xanthine oxidase (XO) is the enzyme that converts hypoxanthine or xanthine into uric acid, a reaction that also gene...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2013.864667
更新日期:2014-01-01 00:00:00
abstract::As a result of strong experimental data supporting effectiveness and safety, herb-based immunomodulators are paving way as alternative sources of potent adjuvants for vaccines. In this study, the immunostimulatory and adjuvant properties of AcF1, a flavonoids-rich fraction of Alchornea cordifolia extract, was evaluate...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820130903496793
更新日期:2010-01-01 00:00:00
abstract::CD44 comprises a family of type I transmembrane glycoproteins that is expressed on a wide range of cells including those of epithelial, lymphoid and myeloid lineage. Although expression of CD44 in the small intestine is typically localised in the crypts of Lieberkuhn, we have reported the expression of CD44 on mature,...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820130009060867
更新日期:2000-08-01 00:00:00
abstract::DNA vaccine represents a powerful approach for prevention of avian H5N1 influenza infection. Yet, DNA vaccine-induced immune responses might be limited by the short duration of antigen expression. As a strategy to enhance adaptive immune responses elicited by a hemagglutinin 5 (H5) DNA vaccine, we explored the effect ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2014.988718
更新日期:2015-01-01 00:00:00
abstract::Jeevaneeya rasayana is an ayurvedic polyherbal formulation, with antirheumatic potential. The present study investigates the therapeutic efficacy of isolated total alkaloid fraction of Jeevaneeya Rasayana (AJR) in treating rheumatoid arthritis in a rat model of Adjuvant-induced arthritis (AIA). Paw swelling, inflammat...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2014.936937
更新日期:2015-01-01 00:00:00
abstract::Prenatal mortality due to loss of lymphocyte-promoted endometrial angiogenesis is being investigated as a major cause of litter reductions during pregnancy in pigs. This review discusses immune mechanisms influencing porcine endometrial angiogenesis as well as additional signalling molecules that may play important ro...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.1080/08820130802191540
更新日期:2008-01-01 00:00:00
abstract::Human tumors often progress and spread in spite of the presence of large numbers of CD4+ and CD8+ T cells with activated or memory cell phenotypes. The T cells in the microenvironment of human lung tumors fail to be activated in response to stimulation via the T cell receptor and CD28 under conditions that fully activ...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.1080/08820130600755066
更新日期:2006-01-01 00:00:00
abstract::Plasmodium falciparum CTL epitope minigenes containing HLA-A2 and HLA-B7 subtype supermotifs were cloned into a plasmid expression vector; this expression was measured in eight human HLA class I molecule specific cell lines. Three assays for in vitro antigen presentation analysis were developed to examine the cross-bi...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-120019206
更新日期:2003-02-01 00:00:00
abstract::Monocyte chemoattractant protein 1 (MCP-1) plays an important role in the development of periodontitis. The purpose of this investigation was to evaluate association of the MCP-1 gene polymorphism with chronic periodontitis in patients with end-stage renal disease (ESRD). One hundred fifty ESRD patients with chronic ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1702052
更新日期:2020-11-01 00:00:00
abstract::Anti-IgM stimulation of B cells is decreased in the presence of maternal serum as compared to control media. This inhibiting influence of maternal serum is observed during the priming of the B cells. The progression of B cells into cellular proliferation was not influenced by maternal serum. At the level of the immuno...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139109026231
更新日期:1991-06-01 00:00:00
abstract::Using antigen-induced proliferation assay the presence of macrophages was found to be essential for the development of the T cell response. Decreased ability of antigen presentation by macrophages from aged A/J mice was detected. ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138509042005
更新日期:1985-04-01 00:00:00
abstract::The induction of immune tolerance is the most common consequence of protein feeding, i.e., "oral tolerance". In this study we investigated the genetic basis of oral tolerance using various kinds of recombinant and congenic mice, and the cells involved in the development of this phenomenon in mice. The footpad swelling...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139309063423
更新日期:1993-08-01 00:00:00
abstract::The role of innate immunity, e.g., complement activation and cytokine release in the hemodynamic alterations in the course of human septic shock is largely unknown. We prospectively studied 14 consecutive septic shock patients with a pulmonary artery catheter in place. For 3 days after admission, hemodynamic variables...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2010.502949
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:Rheumatic fever (RF) and its sequel rheumatic heart disease (RHD) is an autoimmune disease caused by an abnormal host immune response to group A streptococcus (GAS) infection. The HLA class II molecules are entailed in immune-mediated infectious, inflammatory, and autoimmune diseases including RHD. However, ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2020.1822864
更新日期:2020-09-23 00:00:00
abstract::In the last 10 years many of the superantigens of the microbial world have been defined and the mechanisms of cellular interaction between lymphocytes and antigen presenting cells has been elucidated in great detail. The consequences of superantigen stimulation of the immune system, though less well defined, can be co...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139709048933
更新日期:1997-01-01 00:00:00
abstract::To investigate whether phospholipase D1 (PLD1) is involved in autoimmune damage to the eyes, we used Western blotting and immunohistochemistry to examine the expression and distribution of PLD1 in the retinas of Lewis rats with experimental autoimmune uveoretinitis (EAU). Western blot analysis showed that the level of...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Interleukin (IL)-10 response is associated with mortality in patients with sepsis. IL-10 is primarily produced by monocytes and type 2 T helper (Th2) cells. The aim of this study was to investigate differences in IL-10 production between monocytes and Th2 cells in patients with sepsis. Forty patients with sepsis and 3...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1288237
更新日期:2017-05-01 00:00:00
abstract:OBJECTIVE:This study aimed to examine the association of three functional IRF5 rs10954213, rs3757385, and rs41298401 polymorphisms with susceptibility to unexplained recurrent pregnancy loss (RPL) among Iranian women from south of Iran. METHODS:176 women with unexplained RPL and 173 healthy postmenopausal controls wer...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1225759
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Adult chronic immune thrombocytopenic purpura (chronic ITP) is an autoimmune multifactorial bleeding disorder that occurs because of enhanced peripheral platelet destruction. Treatment decisions can be challenging because the goal of treatment is to prevent severe bleeding, but the risk of bleeding can be di...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2015.1064948
更新日期:2015-01-01 00:00:00
abstract::Exposure to laboratory animals poses a hazard for development of occupational allergy. Identification of antigenic determinants of allergenic proteins may be valuable for immunotherapeutic purposes. Overlapping peptides of the major allergen in rat urine, Rat n 1.02, corresponding to the protein alpha2u-globulin were ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139909062266
更新日期:1999-09-01 00:00:00
abstract::The relationships between polymorphisms in pro-inflammatory cytokines and the risk of inflammatory bowel disease (IBD) remain discrepant. Therefore, the authors conducted a meta-analysis to robustly explore relationships between polymorphisms in pro-inflammatory cytokines and the risk of IBD by integrating the results...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2020.1787438
更新日期:2020-08-26 00:00:00