Abstract:
:The present study examined the utility of an immunoblot method for quantitation of cytochrome P450 isozymes in archived liver samples as a bioassay of exposure to halogenated hydrocarbons. Hepatic microsomes were prepared from 44 archived polar bear (Ursus maritimus) liver homogenates that had been stored at approximately -40 degrees C for 9-10 years and analyzed on blots probed with antibodies to rat cytochromes P450 1A1 and P450 2B1. The results revealed a positive correlation between cytochrome P450 1A and total polychlorinated biphenyl (PCB) levels in the archived liver samples, suggesting that cytochrome P450 1A was induced in polar bears by environmental exposure to PCBs.
journal_name
Chemospherejournal_title
Chemosphereauthors
Bandiera SM,Torok SM,Letcher RJ,Norstrom RJdoi
10.1016/s0045-6535(97)00443-8subject
Has Abstractpub_date
1997-03-01 00:00:00pages
1469-79issue
5-7eissn
0045-6535issn
1879-1298pii
S0045-6535(97)00443-8journal_volume
34pub_type
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