Immunoquantitation of cytochromes P450 1A and P450 2B and comparison with chlorinated hydrocarbon levels in archived polar bear liver samples.

Abstract:

:The present study examined the utility of an immunoblot method for quantitation of cytochrome P450 isozymes in archived liver samples as a bioassay of exposure to halogenated hydrocarbons. Hepatic microsomes were prepared from 44 archived polar bear (Ursus maritimus) liver homogenates that had been stored at approximately -40 degrees C for 9-10 years and analyzed on blots probed with antibodies to rat cytochromes P450 1A1 and P450 2B1. The results revealed a positive correlation between cytochrome P450 1A and total polychlorinated biphenyl (PCB) levels in the archived liver samples, suggesting that cytochrome P450 1A was induced in polar bears by environmental exposure to PCBs.

journal_name

Chemosphere

journal_title

Chemosphere

authors

Bandiera SM,Torok SM,Letcher RJ,Norstrom RJ

doi

10.1016/s0045-6535(97)00443-8

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

1469-79

issue

5-7

eissn

0045-6535

issn

1879-1298

pii

S0045-6535(97)00443-8

journal_volume

34

pub_type

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