MR imaging and scintigraphy of gene expression through melanin induction.

Abstract:

PURPOSE:To determine whether an expression vector that encodes for human tyrosinase, the key enzyme in the melanin production pathway, can be used to image gene expression with magnetic resonance (MR) imaging and scintigraphy. MATERIALS AND METHODS:Mouse fibroblasts and human embryonal kidney cells were transfected with an expression vector that contained a complete complementary DNA sequence that encodes the human tyrosinase gene (pcDNA3tyr). Transfected cells were assayed for messenger RNA presence, melanin staining, and indium-111 binding; scintigraphy and MR imaging were performed. RESULTS:Transfected cells contained tyrosinase messenger RNA and stained positively for melanin. Transfected cells had a higher In-111 binding capacity than nontransfected cells, a difference readily detectable with scintigraphy. MR imaging showed transfected cells to have markedly higher signal intensity after gene transfer than nontransfected cells. CONCLUSION:Gene transfer and expression in cell culture can be detected with MR imaging and scintigraphy. The proposed strategy of using an imaging marker gene may have a substantial effect on the noninvasive imaging of gene therapy.

journal_name

Radiology

journal_title

Radiology

authors

Weissleder R,Simonova M,Bogdanova A,Bredow S,Enochs WS,Bogdanov A Jr

doi

10.1148/radiology.204.2.9240530

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

425-9

issue

2

eissn

0033-8419

issn

1527-1315

journal_volume

204

pub_type

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