Abstract:
:Feline bone marrow cells treated with the soybean agglutinin (SBA) lectin are separated into two populations, the agglutinated SBA(+) fraction containing predominantly cells of myeloid origin and the nonagglutinated SBA(-) fraction consisting of cells primarily of the erythroid lineage. FACScan analyses revealed a clear distinction of the cells based on their light scattering properties, i.e., large cells and cells with high granularity were found in the SBA(+) fraction, whereas cells having a low forward light scatter and side light scatter were found in the SBA(-) fraction. Colony-forming assays showed colony-forming unit-granulocyte/monocyte (CFU-GM) cells to have a strong affinity for SBA because these were found almost entirely in the SBA(+) fraction; in contrast, burst-forming unit-erythroid (BFU-E)-forming cells were concentrated in the SBA(-) fraction. When the marrow was fractionated by counterflow centrifugal elutriation (CCE), a differential binding to SBA among the CFU-GM forming cells was found. The SBA(-) fractions of cells collected at 21 and 25 ml/min contained primarily BFU-E forming cells, similar to that observed with whole marrow; the later CCE fractions, those collected at 32 ml/min and the rotor off fraction, when treated with SBA showed a small but significant number of CFU-GM cells in the SBA(-) fraction. T lymphocytes were found predominantly in the SBA(+) fractions of whole bone marrow and the CCE fractions. Successful autologous marrow transplants were performed with the early CCE SBA(-) fractions. The latter cells were used for our initial transplant attempts because ongoing studies in our laboratory had shown these cells to be free of any viral-containing cells when the marrow had been obtained from animals infected with the feline immunodeficiency virus. In summary, although SBA treatment of feline marrow yields a marked separation of CFU-GM and BFU-E progenitors, select CCE SBA(-) fractions contain stem cells capable of providing hematopoietic reconstitution of lethally irradiated animals.
journal_name
Transplantationjournal_title
Transplantationauthors
Gengozian N,Reyes L,Pu R,Homer BL,Bova FJ,Yamamoto JKdoi
10.1097/00007890-199708150-00022subject
Has Abstractpub_date
1997-08-15 00:00:00pages
510-8issue
3eissn
0041-1337issn
1534-6080journal_volume
64pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:CD4+ T cells play an essential role in allograft rejection. The monoclonal anti-rat CD4 antibody, RIB 5/2, has been shown to modulate the CD4 glycoprotein without eliminating the recipient T cells. We have successfully induced tolerance to rat heart allografts by recipient pretreatment with a single dose of ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200001270-00015
更新日期:2000-01-27 00:00:00
abstract::We are participating in a multicenter trial testing the efficacy of a murine monoclonal antihuman peripheral T lymphocyte antibody (OKT3.PAN) as immunosuppressive therapy for the treatment of acute cadaveric renal allograft rejection. Although clinical data indicate that administration of this antibody clears the circ...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:10.1097/00007890-198411000-00009
更新日期:1984-11-01 00:00:00
abstract:BACKGROUND:The potential benefits of islet xenografting in type 1 diabetes include the intriguing, but still unanswered, possibility that the grafted xenoislets may be less subjected to human autoimmune attack. Cytokines may play a major role in the pathogenesis of autoimmune diabetes by causing impairment of insulin r...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200101150-00004
更新日期:2001-01-15 00:00:00
abstract:OBJECTIVE:The aim of this prospective, multicenter, noncomparative, open-label trial was to evaluate the prophylactic use of caspofungin in adult liver transplant recipients at high risk of developing invasive fungal infections (IFI). METHODS:Patients received caspofungin for at least 21 days. A successful treatment o...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究
doi:10.1097/TP.0b013e3181932e76
更新日期:2009-02-15 00:00:00
abstract:BACKGROUND:The impact of community-acquired respiratory virus (CARV) infections on bronchiolitis obliterans syndrome (BOS) and outcome after lung transplantation (LTx) and diagnostic techniques were prospectively evaluated. METHODS:A single-center prospective cohort study was performed in LTx-outpatients between Octob...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181a4857d
更新日期:2009-05-27 00:00:00
abstract:BACKGROUND:Gene targeting in large animals has the potential to be useful in medicine as well as in agriculture. Previously, we reported the first successful targeting of the bovine alpha1,3-galactosyltransferase (alpha1,3GT) gene and establishment of a heterozygous knockout cell line. In this report, we generated both...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000190422.66657.f1
更新日期:2006-03-15 00:00:00
abstract:BACKGROUND:We previously reported that no cytotoxic T-lymphocyte precursor frequencies (CTLpf) were found in 60% of patients on azathioprine or mycophenolate mofetil+Pred long after kidney transplantation. We questioned whether the absence of donor-specific CTLpf was associated with low levels of stimulatory Th1 (tumor...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181a36c85
更新日期:2009-05-27 00:00:00
abstract:BACKGROUND:This study investigated the use of deceased heart-beating donor livers offered for transplantation during a 10-year period, during which there has been an increasing disparity between organ supply and demand in the United Kingdom. METHODS:Summary data from the National Transplant Database were analyzed on a...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000268072.04260.69
更新日期:2007-07-15 00:00:00
abstract:BACKGROUND:Lung transplant recipients are among those with the highest risk of influenza infection and complications each year. A few studies show adequate responses after influenza immunization; no studies examined the season-long protection. METHODS:Influenza antibody concentrations were measured using hemagglutinat...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000391
更新日期:2015-04-01 00:00:00
abstract::The humoral theory states that antibodies cause the rejection of allografts. From 1917 to 1929, extensive efforts were made to produce antibodies against tumors. It was finally realized that the antibodies were produced against the transplant antigens present on transplantable tumors, not against the tumor-specific an...
journal_title:Transplantation
pub_type: 历史文章,杂志文章
doi:10.1097/TP.0b013e3182483713
更新日期:2012-04-27 00:00:00
abstract::Immunosuppressive therapy increases the risk of recurrence of initial cancers in organ transplant patients, and compelling therapeutic protocols are needed to suppress the malignancy and protect the allograft. We examined the potential use of 15-deoxyspergualin (DSG) in relation to organ transplantation and cancer. Th...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000271496.75233.f6
更新日期:2007-08-15 00:00:00
abstract::The immunosuppressive effects of leflunomide and cyclosporine were evaluated in a rat neurovascularized myocutaneous allograft model. Inbred Brown-Norway and Lewis rats were served as donors and recipients, respectively. All recipients were observed for 60 days or until allograft rejection occurred. All isograft contr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199609270-00027
更新日期:1996-09-27 00:00:00
abstract:BACKGROUND:The current shortage of organ donors has led many centers to use marginal and nonheart-beating donors (NHBDs). Recent research has implicated the infiltration of lymphocytes as an important mediator of ischemia-reperfusion injury (IRI). FTY720 is an immunosuppressant that promotes lymphocyte sequestration in...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000269794.74990.da
更新日期:2007-07-27 00:00:00
abstract:BACKGROUND:Kidney transplantation (KT) of human immunodeficiency virus (HIV)-positive patients has transformed the management of end-stage kidney disease in this population. Although favourable outcomes have been reported, patients experience high rates of acute allograft rejection (AR). We examined factors associated ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000879
更新日期:2016-04-01 00:00:00
abstract::LEW rats with long-surviving (> 100 days) (DA x LEW)F1 kidney allografts were generated by treating the recipients with cyclosporine for 14 days after grafting. All rats were monitored after transplantation for the development of antibodies to intact donor class I MHC molecules. Cyclosporine completely suppressed the ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199605270-00007
更新日期:1996-05-27 00:00:00
abstract:BACKGROUND:We herein investigate critical ischemia times and the effect of novel preservation solutions such as new histidine-tryptophan-ketoglutarate (HTK-N) and TiProtec on the individual tissues of a rat limb isograft. METHODS:Orthotopic hind-limb transplantations were performed in male Lewis rats after 2 hours, 6 ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001845
更新日期:2017-09-01 00:00:00
abstract::When normal mice are exposed for short periods to ultraviolet light (UV), they support the progressive growth of transplanted syngeneic UV-induced tumors. Normal nonirradiated mice almost always reject these tumor implants. The UV-mediated suppression of the antitumor response can be adoptively transferred to normal s...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197708000-00005
更新日期:1977-08-01 00:00:00
abstract:BACKGROUND:Human leukocyte antigen (HLA) antibodies produced after transplantation are frequently measured in transplant recipients, because they are strongly associated with humoral rejection and graft loss. However, antibodies can be induced by posttransplant blood transfusions rather than by the graft, casting doubt...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181a24b96
更新日期:2009-05-15 00:00:00
abstract:INTRODUCTION:The importance of HLA matching for renal transplantation outcomes has been appreciated for several decades. It has been hypothesized that as pharmacologic immunosuppression becomes stronger and more specific, the impact of HLA matching may be vanishing. Mycophenolate Mofetil (MMF) has been demonstrated to ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200102150-00010
更新日期:2001-02-15 00:00:00
abstract::Discordant xenogeneic organ transplantation is a potential solution to the critical shortage of suitable donor organs. However, clinical application of xenotransplantation with physiologically suitable organs such as those from the pig, is currently limited by the lack of agents to prevent antibody and complement-medi...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-05-27 00:00:00
abstract::The renal handling of cyclosporine was studied in ischemically damaged kidneys in New Zealand White rabbits and nonischemic control animals. CsA, 25 mg/kg/day, was administered intravenously for 10 days starting with the day of operation. Blood CsA (B CsA) was higher in the ischemic group compared with the controls (m...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199003000-00004
更新日期:1990-03-01 00:00:00
abstract::Leflunomide is a compound recently shown to reduce T and B cell-mediated responses in a number of experimental rat, mouse, and human systems. To explore its potential as an immunosuppressant, we studied leflunomide in 128 Brown-Norway/Lewis cardiac transplants and in 48 unoperated Lewis rats. At doses ranging from 0.6...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-04-27 00:00:00
abstract::Cyclosporine treatment of BALB/c mice (at a dose of 20 mg/kg every other day for 3 weeks) caused a remarkable reduction in the PNA-, L3T4+Lyt-2- subset of thymocytes. A significant reduction of the L3T4+Lyt-2-subset was also observed in both the lymph node and spleen cells of CsA-treated mice, though the degree of the...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198903000-00020
更新日期:1989-03-01 00:00:00
abstract:BACKGROUND:New-onset diabetes is common after kidney transplantation, but the benefit of lifestyle intervention to improve glucose metabolism posttransplantation is unproven. METHODS:We conducted a single-center, randomized controlled trial involving 130 nondiabetic kidney transplant recipients with stable function be...
journal_title:Transplantation
pub_type: 杂志文章,随机对照试验
doi:10.1097/TP.0000000000002969
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:A recent proposal supports the elimination of allocation points for human leukocyte antigen (HLA) mismatches (MM) in cadaveric kidney transplantation. The intent is to increase access for some racial groups that might be disadvantaged by the representation of race-specific HLA in a largely white donor pool. ...
journal_title:Transplantation
pub_type: 评论,杂志文章
doi:10.1097/00007890-200212150-00024
更新日期:2002-12-15 00:00:00
abstract::In a cross-sectional survey of the quality of life of 45 liver transplant recipients, physical and psychological status, physical complaints, capability to participate in daily life, social support, and global quality of life were assessed. The average time of follow-up was 9 months after transplantation. Nine patient...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199309000-00013
更新日期:1993-09-01 00:00:00
abstract:BACKGROUND:Blockade of the inducible costimulator (ICOS) pathway has been shown to prolong allograft survival; however, its utility in xenotransplantation is unknown. We hypothesize that local expression of ICOS-Ig by the xenograft will suppress the T-cell response resulting in significant prolonged graft survival. ME...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31821774e0
更新日期:2011-05-27 00:00:00
abstract:BACKGROUND:Patients with high tacrolimus clearance eliminate more drug within a dose interval compared with those with low clearance. Delays in dosing time will result in transient periods of lower concentrations in high versus low clearance patients. Transient subtherapeutic tacrolimus concentrations may induce acute ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001796
更新日期:2017-08-01 00:00:00
abstract::Despite the significant improvements that have occurred since the introduction of CsA, long-term renal allograft survival continues to be an area of concern. Management strategies that involve the use of sirolimus offer some promise. A number of observations suggest that sirolimus may have the ability to reduce the ra...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:
更新日期:2001-06-15 00:00:00
abstract:BACKGROUND:Preliminary studies showed an excellent success rate of kidney grafts in patients with high pretransplantation serum levels of IgA autoantibodies directed against the Fab region of the human IgG molecule. METHODS:With the collaboration of 30 centers from around the world, we attempted to verify the role of ...
journal_title:Transplantation
pub_type: 评论,杂志文章,多中心研究
doi:10.1097/00007890-200004150-00021
更新日期:2000-04-15 00:00:00