Recombinant human transforming growth factor beta 3 accelerates gastric ulcer healing in rats.

Abstract:

BACKGROUND:Gastric ulcer healing is mediated by various endogenous growth factors. In this experimental study effect of locally and systemically applied recombinant human transforming growth factor beta 3 (rhTGF-beta 3) on gastric ulcer healing was investigated in the rat. METHODS AND RESULTS:Gastric ulcers were induced with a cryoprobe, and ulcer healing was evaluated 7 days after local infiltration (0.5 micrograms, 1.0 microgram, 2.5 micrograms, and 50 micrograms) or systemic (intravenous) application of TGF-beta 3 (500 micrograms/kg body weight). Compared with controls, a dose-dependent stimulation of ulcer healing (as evidenced by a reduction in ulcer size) was observed 7 days after local infiltration of TGF-beta 3 (1.0 microgram, 2.5 micrograms, and 50 micrograms). Corresponding increases in the levels of proliferating cell nuclear antigen (PCNA) and intracellular TGF-beta 3 expression and a downregulation of the TGF-beta type-II receptor expression were also observed in the granulation tissue of the ulcer margins. Systemic application of TGF-beta 3 produced effects similar to those observed after local treatment with 50 micrograms of the compound. CONCLUSION:Local and systemic TGF-beta 3 treatment accelerates gastric ulcer healing in rats.

journal_name

Scand J Gastroenterol

authors

Coerper S,Sigloch E,Cox D,Starlinger M,Köveker G,Becker HD

doi

10.3109/00365529709011214

subject

Has Abstract

pub_date

1997-10-01 00:00:00

pages

985-90

issue

10

eissn

0036-5521

issn

1502-7708

journal_volume

32

pub_type

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